Presynaptic failure of neuromuscular propagation in rats

Krnjević, K.; Miledi, Ricardo

doi:10.1113/jphysiol.1959.sp006321

Cited by 287 publications

(184 citation statements)

References 54 publications

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“…entry, such as Ca ?? channel inactivation (Brehm and Eckert 1978), autoreceptor activation (Nistri and Cherubini 1991;Wu and Saggau 1994) or action potential conduction failures (Krnjevic and Miledi 1958), are unlikely to significantly contribute to the depression in this study.…”

Section: Discussionmentioning

confidence: 95%

Synaptic activity slows vesicular replenishment at excitatory synapses of rat hippocampus

Bui

Glavinović

2012

Cogn Neurodyn

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Short-term synaptic depression mainly reflects the depletion of the readily releasable pool (RRP) of quanta. Its dynamics, and especially the replenishment rate of the RRP, are still not well characterized in spite of decades of investigation. Main reason is that the vesicular storage and release system is treated as time-independent. If it is time-dependent all parameters thus estimated become problematic. Indeed the reports about how prolonged stimulation affects the dynamics are contradictory. To study this, we used patterned stimulation on the Schaeffer collateral fiber pathway and model-fitting of the excitatory post-synaptic currents (EPSC) recorded from CA1 neurons in rat hippocampal slices. The parameters of a vesicular storage and release model with two pools were estimated by minimizing the squared difference between the ESPC amplitudes and simulated model output. This yields the 'basic' parameters (release coupling, replenishment coupling and RRP size) that underlie the 'derived' and commonly used parameters (fractional release and replenishment rate). The fractional release increases when [Ca ?? ] o is raised, whereas the replenishment rate is [Ca ?? ] o independent. Fractional release rises because release coupling increases, and the RRP becomes less able to contain quanta. During prolonged stimulation, the fractional release remains generally unaltered, whereas the replenishment rate decreases down to *10 % of its initial value with a decay time of *15 s, and this decrease in the replenishment rate significantly contributes to synaptic depression. In conclusion, the fractional release is [Ca ?? ] odependent and stimulation-independent, whereas the replenishment rate is [Ca ?? ] o -independent and stimulation-dependent.

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Section: Discussionmentioning

confidence: 95%

Synaptic activity slows vesicular replenishment at excitatory synapses of rat hippocampus

Bui

Glavinović

2012

Cogn Neurodyn

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“…When the action potential fails to propagate along the axon, no signal can reach the output of the cell. Conduction failure filters communication with postsynaptic neurons, and has been observed experimentally in various axons including vertebrate spinal axons 68,69 , spiny lobster or crayfish motor neurons 33,64,70,71 , leech mechanosensory neurons [72][73][74][75][76] , thalamocortical axons 77 , rabbit nodose ganglion neurons 78 , rat dorsal root ganglion neurons 63,79 , neurohypophysial axons 17,80 and hippocampal pyramidal cells 39,66,81 . Several factors determine whether propagation along axons fails or succeeds.…”

Section: Propagation Failuresmentioning

confidence: 99%

“…In most cases, failures occur following moderate or high frequency stimulation (10-50 Hz) of the axon. For instance, a frequency of 20-30 Hz is sufficient to produce conduction failures at the neuromuscular terminal arborization 69 or at the branch point of spiny lobster motor neurons 33 . These failures are often observed as partial spikes or spikelets that are electrotonic residues of full action potentials.…”

Section: Axonal Propagation and Spike Timingmentioning

confidence: 99%

Information processing in the axon

2004

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“…formation, presumably as a result of blockage of invasion of the motor nerve terminals by the action potential (cf. Krnjevi6 & Miledi, 1959;Hubbard & L0yning, 1966). Fig.…”

mentioning

confidence: 99%

On the role of mitochondria in transmitter release from motor nerve terminals.

Alnæs¹,

Rahamimoff²

1975

The Journal of Physiology

381

133

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4. The mitochondrial content of the motor nerve terminals is, on the average, 6-59% 5. The experimental results are explained on the hypothesis that spontaneous release of transmitter reflects the resting level of intracellular free calcium and the evoked release reflects the sum of the resting calcium and the calcium brought in by the action potential. The mitochondria play a role in transmitter release by participating in the regulation of the intracellular free Ca.

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Presynaptic failure of neuromuscular propagation in rats

Cited by 287 publications

References 54 publications

Synaptic activity slows vesicular replenishment at excitatory synapses of rat hippocampus

Synaptic activity slows vesicular replenishment at excitatory synapses of rat hippocampus

Information processing in the axon

On the role of mitochondria in transmitter release from motor nerve terminals.

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