Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit

Tabor, Kathryn M.; Smith, Trevor; Brown, Mary Elizabeth; Bergeron, Sadie A.; Briggman, Kevin L.; Burgess, Harold A.

doi:10.1016/j.cub.2018.06.020

Cited by 62 publications

(85 citation statements)

References 60 publications

(116 reference statements)

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“…We co-injected 50 ng of UAS:nls-GCaMP6s construct (derived from a previously published plasmid UAS:nls-GCaMP6s-2a-nls-dsRed (Tabor et al, 2018)) with 80 ng tol1 RNA into one cell stage y279:Gal4 embryos, and raised in medium containing 200 µM PTU. At 6 dpf larvae were mounted in 2% low melting temp agarose and imaged using a 20x immersion objective on an upright Leica TCS-SP5II microscope.…”

Section: Calcium Imagingmentioning

confidence: 99%

Molecular and cellular determinants of motor asymmetry in zebrafish

Horstick

Bayleyen

Burgess

2019

Preprint

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Asymmetries in motor behavior, such as human hand preference, are observed throughout bilateria.However, neural substrates and developmental signaling pathways that impose underlying functional lateralization on a broadly symmetric nervous system are unknown. Here we report that in the absence of over-riding visual information, zebrafish larvae show intrinsic lateralized motor behavior that is mediated by a cluster of 60 posterior tuberculum (PT) neurons in the forebrain. PT neurons impose motor bias via a projection through the epithalamic commissure to the habenula. Acquisition of left/right identity is disrupted by heterozygous mutations in mosaic eyes and mindbomb, genes that regulate Notch signaling. These results define the neuronal substrate for motor asymmetry in a vertebrate and support the idea that developmental pathways that establish visceral asymmetries also govern acquisition of left/right identity. Results Larval zebrafish show persistent individual lateralized behavior in locomotor trajectoriesAfter loss of illumination, 6 days post-fertilization (dpf) larval zebrafish initiate a circular swimming behavior in which they repeatedly perform same-direction turn maneuvers in a restricted spatial area ( Figure 1A) (Horstick et al., 2017). Same-direction turn movements were sustained in individual larvae for two minutes (Figure 1B), however across the population there was no net tendency for larvae to

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Section: Calcium Imagingmentioning

confidence: 99%

Molecular and cellular determinants of motor asymmetry in zebrafish

Horstick

Bayleyen

Burgess

2019

Preprint

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“…Consequently, in clutches from et-Cre;βactin:Switch crossed to βactin:Switch, we discarded ~25% of embryos that showed ubiquitous RFP expression due to complete recombination of the Switch reporter in gametes also containing the Cre transgene. We also imaged previously described Cre lines with rhombomere- (Tabor et al, 2018). Gal4 enhancer trap lines were isolated as previously described (Bergeron et al, 2012).…”

Section: Zebrafish Linesmentioning

confidence: 99%

“…In zebrafish, intersectional control of transgene expression has been achieved by combining the Gal4/UAS and Cre/lox systems (Forster et al, 2017;Satou et al, 2013;Tabor et al, 2018). Gal4-Cre intersectional systems take advantage of hundreds of existing Gal4 lines which are already widely used in zebrafish circuit neuroscience (Asakawa et al, 2008;Bergeron et al, 2012;Scott et al, 2007), but are limited by the relatively poor repertoire of existing Cre lines, and difficulty in identifying pairs of driver lines that co-express in neurons of interest.…”

Section: Introductionmentioning

confidence: 99%

“…However, Cre lines provide limited access to the midbrain tegmentum, posterior tuberculum and trigeminal ganglion (Fig. The ZBB2 atlas provides cellular resolution imaging data for 65 Cre lines, and 158 Gal4 lines, enabling small clusters of neurons to be genetically addressed through intersectional targeting. To aid in the design of such experiments, we have implemented a web-based interface that allows users to perform a spatial search for lines that express Cre or Gal4 in any selected 3D volume, and thereby identify transgenic lines for intersectional visualization or manipulation of selected neurons (Tabor et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Several intersectional reporter lines have already been generated to visualize cells that co-express Cre and Gal4 (Forster et al, 2017;Satou et al, 2013). In addition, the UAS:KillSwitch line enables selective ablation of neurons that coexpress Gal4 and Cre, and the UAS:DoubleSwitch line sparsely labels neurons within a Gal4/Cre coexpression domain for morphological reconstruction (Tabor et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

Tabor¹,

Marquart

Hurt

et al. 2018

Preprint

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Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies. design of intersectional genetic experiments. However, ZBB software required local installation and only included 9 Cre lines, limiting opportunities for intersectional targeting.Here, we describe the ZBB2 atlas, which comprises whole-brain expression patterns for 264 transgenic lines, including 65 Cre lines, and 158 Gal4 lines. We generated more than 100 new enhancer trap lines that express Cre or Gal4 in diverse subsets of neurons, then registered a high resolution image of each to the original ZBB atlas. For 3D visualization of expression patterns and to facilitate spatial searches for experimentally useful lines, we now provide an online interface to the atlas. Collectively, ZBB2 labels almost all cellular regions within the brain and will facilitate the reproducible targeting of neuronal subsets for circuit-mapping studies. ResultsTo accelerate discovery of functional circuits we generated a library of transgenic lines that express Cre in restricted patterns within the brain and built an online 3D atlas ( Fig. 1). New Cre lines were generated through an enhancer trap screen: we injected embryos with a Cre vector containing a basal promoter that includes a neuronal-restrictive silencing element to suppress expression outside the nervous system, and tol2 transposon arms for high efficiency transgenesis (REx2-SCP1:BGi-Cre-2a-Cer) (Bergeron et al., 2012;Kawakami, 2007;Marquart et al., 2015). In injected G0 embryos, the reporter randomly integrates into the genome such that Cre expression is directed by local enhancer elements. To isolate lines with robust brain expression in relatively restricted domains, we visually screened progeny of G0 adults crossed to the βactin:Switch transgenic line that expresses red fluorescent protein (RFP) in cells with Cre (Horstick et al., 2014). We retained 52 new lines that express Cre in restricted brain regions at 6 days post-fertilization (dpf), the stage most frequently used 4B). Gal4 lines tend to have more restricted expression than Cre lines, with a median coverage of 1% (range 0.02 to 29%, ~900 neurons per line). Collectively Gal4 lines label 91% of the cell body vol...

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Gsx2, but not Gsx1, is necessary for early forebrain patterning and long‐term survival in zebrafish

Coltogirone

Sherfinski

Dobler

et al. 2022

Developmental Dynamics

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Background Homeobox transcription factor encoding genes, genomic screen homeobox 1 and 2 (gsx1 and gsx2), are expressed during neurodevelopment in multiple vertebrates. However, we have limited knowledge of the dynamic expression of these genes through developmental time and the gene networks that they regulate in zebrafish. Results We confirmed that gsx1 is expressed initially in the hindbrain and diencephalon and later in the optic tectum, pretectum, and cerebellar plate. gsx2 is expressed in the early telencephalon and later in the pallium and olfactory bulb. gsx1 and gsx2 are co‐expressed in the hypothalamus, preoptic area, and hindbrain, however, rarely co‐localize in the same cells. gsx1 and gsx2 mutant zebrafish were made with TALENs. gsx1 mutants exhibit stunted growth, however, they survive to adulthood and are fertile. gsx2 mutants experience swim bladder inflation failure that prevents survival. We also observed significantly reduced expression of multiple forebrain patterning distal‐less homeobox genes in mutants, and expression of foxp2 was not significantly affected. Conclusions This work provides novel tools with which other target genes and functions of Gsx1 and Gsx2 can be characterized across the central nervous system to better understand the unique and overlapping roles of these highly conserved transcription factors.

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Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit

Cited by 62 publications

References 60 publications

Molecular and cellular determinants of motor asymmetry in zebrafish

Molecular and cellular determinants of motor asymmetry in zebrafish

Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

Gsx2, but not Gsx1, is necessary for early forebrain patterning and long‐term survival in zebrafish

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