Preterm prelabor rupture of membranes without microbial invasion of the amniotic cavity and intra-amniotic inflammation: a heterogeneous group with differences in adverse outcomes

Kacerovský, Marian; Matulova, Jana; Andrýs, Ctirad; Mls, Jan; Hornychová, Helena; Kukla, Rudolf; Boštík, Pavel; Burčková, Hana; Špaček, Richard; Jacobsson, Bo; Musilová, Ivana

doi:10.1080/14767058.2021.2017875

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Amniotic fluid samples, cervical fluid samples, and the clinical and demographic data of some women from this cohort were used in our previous studies ( Musilova et al, 2017a ; Musilova et al, 2017b ; Hornychova et al, 2018 ; Kacerovsky et al, 2018 ; Musilova et al, 2018 ; Janku et al, 2019 ; Kacerovsky et al, 2020a ; Kacerovsky et al, 2020b ; Musilova et al, 2020 ; Soucek et al, 2020 ; Spacek et al, 2020 ; Kacerovsky et al, 2021a ; Kacerovsky et al, 2021b ; Matulova et al, 2021 ; Musilova et al, 2021 ; Stranik et al, 2021 )…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks

et al. 2022

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Objectives: To determine the prevalence and load of Ureaplasma spp. DNA in the cervical fluid of women with singleton pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to intra-amniotic infection, sterile intra-amniotic inflammation, and colonization of the amniotic fluid.Methods: A total of 217 women with PPROM between gestational ages 24 + 0 and 33 + 6 weeks were included in this study. Paired amniotic and cervical fluid samples were collected at the time of admission via transabdominal amniocentesis and using a Dacron polyester swab, respectively. Microbial invasion of the amniotic cavity was diagnosed using a combination of culture and molecular biology methods. Intra-amniotic inflammation was determined based on the concentration of interleukin-6 in the amniotic fluid. Based on the presence or absence of these conditions, the women were stratified into the following subgroups: intra-amniotic infection (with both), sterile intra-amniotic inflammation (with inflammation only), colonization (with microorganisms only), and negative amniotic fluid (without either). The Ureaplasma spp. DNA load in the cervical fluid was assessed using PCR.Results:Ureaplasma spp. DNA in the cervical fluid was found in 61% (133/217) of the women. Women with negative amniotic had similar prevalence of Ureaplasma spp. DNA in cervical fluid (55%) to those with sterile intra-amniotic inflammation (54%) but lower than those with intra-amniotic infection (73%) and colonization (86%; p < 0.0001). Women with negative amniotic fluid had a lower load of Ureaplasma spp. DNA in their cervical fluid (median: 4.7 × 103 copies of DNA/ml) than those with intra-amniotic infection (median: 2.8 × 105 copies DNA/ml), sterile intra-amniotic inflammation (median: 5.3 × 104 copies DNA/ml), and colonization (median: 1.2 × 105 copies DNA/mL; p < 0.0001).Conclusion: In conclusion, in PPROM at <34 weeks, the presence of intra-amniotic infection, sterile intra-amniotic inflammation, or colonization of the amniotic fluid was associated with a higher prevalence and/or load of Ureaplasma spp. DNA in the cervical fluid than the absence of intra-amniotic complications.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks

et al. 2022

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“…Although a higher concentration of inflammatory cytokines was found in the amniotic fluid following delivery [ 11 ], Raba and Tabarkiewicz observed that inflammatory cytokines played a crucial role in over 80% of PTB cases, particularly in those occurring before 30 weeks of gestation [ 12 ]. Other causes of PBT include premature rupture of the membranes caused by an infection of the amniotic sac or alterations in vaginal bacterial flora [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Placental Cannabinoid Receptor Expression in Preterm Birth

Feduniw,

Krupa,

Łagowska

et al. 2024

Journal of Pregnancy

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Background: The cannabinoid receptor (CBR) plays a significant role in oogenesis, pregnancy, and childbirth. It might also play a significant role in preterm birth (PTB). The aim of the study was to investigate the association between the expression of the CBR in the placenta and the incidence of PTB.Methods: This prospective, observational, multicentre preliminary study was conducted on placental samples obtained from 109 women. The study included 95 patients hospitalized due to the high risk of PTB. They were divided into two groups: Group 1, where the expression of the CBR1 and CBR1a was analyzed, and Group 2, in which we examined CBR2 expression. The control group, that is, Group 3, consisted of 14 women who delivered at term, and their placentas were tested for the presence of all three receptor types (CBR1, CBR1a, and CBR2).Results: The study used reverse transcription and real-time PCR methods to assess the expression of CBRs in the placental tissues. The expression of the CBR2, CBR1, and CBR1a receptors was significantly lower in the placentas of women after PTB compared to those after term births, p=0.038, 0.033, and 0.034, respectively.Conclusions: The presence of CBR mRNA in the human placental tissue was confirmed. The decreased expression of CBRs could serve as an indicator in predicting PTB.

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“…Although a higher concentration of inflammatory cytokines was found in the amniotic fluid following delivery [11], Raba et al observed that inflammatory cytokines played a crucial role in over 80% of PTB cases, particularly in those occurring before 30 weeks of gestation [12]. Other causes of PBT include premature rupture of the membranes caused by an infection of the amniotic sac or alterations in vaginal bacterial flora [13,14]. The endocannabinoid signaling system (ECS) includes the cannabinoid receptor type 1 (CBR1) and type 2 (CBR2), along with their endogenous ligands (arachidonoylethanolamide and 2-arachidonoyl glycerol) for the cannabinoid receptors.…”

Section: Introductionmentioning

confidence: 99%

Placental cannabinoid receptor expression in preterm birth - preprint.

Feduniw,

Krupa,

Łagowska

et al. 2024

Preprint

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Endocannabinoid receptor (CB) plays a significant role in oogenesis, pregnancy and childbirth. CB might also play a significant role in preterm birth. The aim of the study was to investigate the association between the expression of cannabinoid receptors within the placenta and preterm delivery (PTB). A prospective, observational, multicentre preliminary study was conducted on placental material obtained from 109 women. The study comprised 95 female patients hospitalized due to preterm delivery, and they were divided into two groups. The expression of CB1 and CB1a was analyzed in Group 1 and CB2 in Group 2. The control group (Group 3) consisted of 14 women who delivered at term, and their placentas were tested for the presence of all three receptors (CB1, CB1a, CB2). The placentas were examined using reverse transcription and Real-Time PCR to assess the expression of cannabinoid receptors in the placentas. The expression of CB2, CB1 and CB1a receptors were significantly lower in placentas from preterm deliveries than term delivery placentas, p = 0.038, 0.033 and 0.034, respectively. Cannabinoid receptor mRNA was identified in human placental tissue. Determining decreased cannabinoid receptor expression in preterm delivery placentas could improve PTB prediction. KEYWORDS: Cannabinoid receptor; CB2; endocannabinoid system; preterm delivery; PTB

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Preterm prelabor rupture of membranes without microbial invasion of the amniotic cavity and intra-amniotic inflammation: a heterogeneous group with differences in adverse outcomes

Cited by 3 publications

References 31 publications

Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks

Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks

Placental Cannabinoid Receptor Expression in Preterm Birth

Placental cannabinoid receptor expression in preterm birth - preprint.

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