Pretreatment Conditions for Hydrolysates from Unbleached Pulp Waste for Ethanol Fermentation with Xylose-fermenting Yeast, &lt;i&gt;Candida intermedia&lt;/i&gt; 4-6-4T2

Saito, Masaru; Nagasaki, Hiroshi; Ikeda, Tomoaki; Saga, Kiyotaka; Yoshida, Kōji; Watanabe, Shigeyuki

doi:10.1627/jpi.61.191

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Hydrolysates from lignocellulosic biomass also contain furan derivatives such as furfural and 5-hydroxymethylfurfural (HMF) as well as acetic acid. We found that furfural and HMF had negligible effects as inhibitors on fermentation using high cell density of C. intermedia 4-6-4T2 (10 g/L (DCW)) 24) . Consequently, we proposed that unidentified inhibitors may be present in hydrolysates pretreated under severe conditions 24) .…”

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confidence: 80%

Effect of Air Sparging on Ethanol Production from Xylose and Glucose in Continuous Chemostat Fermentation Process Utilizing High Cell Density of <i>Candida intermedia</i> 4-6-4T2

Nagasaki

Suzuki

Fujimoto

et al. 2021

J. Jpn. Petrol. Inst.

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Reducing fermentation periods and increasing ethanol productivity are cost effective for ethanol production from lignocellulosic biomass. Increasing the density of cells for fermentation typically increases ethanol productivity, but also increases the concentration of dissolved carbon dioxide (dCO2) in the fermented broth. Such accumulated dCO2 sometimes reduces ethanol production. The Continuous Chemostat Fermentation (CCF) process utilizing high density of Candida intermedia 4-6-4T2 with and without air sparging was evaluated for the effect on ethanol production and rapid fermentation using 24-h cycles. Synthetic fermentation solution without nitrogen sources containing 20 g/L xylose and 30 g/L glucose plus 5 g/L acetic acid as fermentation inhibitor was supplemented into a culture vessel at 15 mL/h, and fermented broth was recovered from the same flask at 15 mL/h. Various conditions were tested to reduce the accumulated dCO2 in the fermented broth, but air sparging at 0.056 vvm was the most effective for ethanol production in the CCF process. For the 24-h startup-batch and 6-cycle CCF process (144 h), the ethanol yield was 0.4 g/g and the cell density of the used C. intermedia 4-6-4T2 for one cycle was one-third compared to that of sequential batch fermentation.

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Section: [Regular Paper]mentioning

confidence: 80%

Effect of Air Sparging on Ethanol Production from Xylose and Glucose in Continuous Chemostat Fermentation Process Utilizing High Cell Density of <i>Candida intermedia</i> 4-6-4T2

Nagasaki

Suzuki

Fujimoto

et al. 2021

J. Jpn. Petrol. Inst.

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Molecular Phylogeny and Phenotypic Characterization of Yeasts with a Broad Range of pH Tolerance Isolated from Natural Aquatic Environments

Urano¹,

Shirao²,

Naito³

et al. 2019

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In this study, yeasts with broad range of pH tolerance were isolated and characterized from natural aquatic environments in Japan. Only a few basic and application studies of alkali-tolerant yeasts have been reported, despite the unmet industrial needs. First, we surveyed alkali-tolerant yeasts from natural aquatic environments at pH 7.6-9.4. We isolated 35 yeast strains that grew in pH 9.0 medium, from seven genera and nine species: 25 strains (N1, N2, through N6, N9, K1, K3 through K19) were Rhodotorula mucilaginosa; one (N7) was Rhodosporidium fluvial; one (N8) was Scheffersomyces spartinae; two (N10 and N13) were Wicherhamonyces anomalus; one (N11) was Cyberlindnera saturnus; one (S1) was Candida sp.; two (S2 and S4) were Candida intermedia; one (S3) was Candida quercuum; and one (K2) was Cryptococcus liquefacience. We examined the effects of pH on the growth of representative yeast strains. Strains K12 and S4 showed high growth at pH 3-10. Strains N7, N8, N10, N11, and S3 showed high growth at pH 3-9. Strains K2 and S1 showed high growth at pH 4-8. All nine of these strains had neutralizing activities from acidic media at pH 3-5 to pH 6-8. We previously isolated acid-tolerant yeasts (Cryptococcus sp. T1 [1] and Candida intermedia CeA16 [2]) from extremely acidified environments; they showed high growth at pH 3-9 and neutralizing activities of acidic media by releasing ammonium ions. Thus, alkali-tolerant yeasts and acid-tolerant yeasts were found to be similar species and have both high growth at a broad range of pH and neutralizing activities of acid media. Previously, we also isolated acid-tolerant, acid-neutralizing yeasts How to cite this paper:

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Pretreatment Conditions for Hydrolysates from Unbleached Pulp Waste for Ethanol Fermentation with Xylose-fermenting Yeast, <i>Candida intermedia</i> 4-6-4T2

Cited by 2 publications

References 16 publications

Effect of Air Sparging on Ethanol Production from Xylose and Glucose in Continuous Chemostat Fermentation Process Utilizing High Cell Density of <i>Candida intermedia</i> 4-6-4T2

Effect of Air Sparging on Ethanol Production from Xylose and Glucose in Continuous Chemostat Fermentation Process Utilizing High Cell Density of <i>Candida intermedia</i> 4-6-4T2

Molecular Phylogeny and Phenotypic Characterization of Yeasts with a Broad Range of pH Tolerance Isolated from Natural Aquatic Environments

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