Pretreatment of in vitro matured bovine oocytes with docetaxel before vitrification: Effects on cytoskeleton integrity and developmental ability after warming

Chasombat, Jakkhaphan; Nagai, Takashi; Parnpai, Rangsun; Vongpralub, Thevin

doi:10.1016/j.cryobiol.2015.07.002

Cited by 15 publications

(7 citation statements)

References 48 publications

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“…In general, FBS concentration in the oocyte recovery media is variable. Chasombat et al (2015) used 10% FBS in bovine oocyte retrieval medium obtaining good blastocyst rate (30.9%) at the end of IVF. On the other hand, Chen et al (2016) used a 2% FBS during the recovery and reached 23.0% blastocysts after 8 days of culture of the embryos generated.…”

Section: Discussionmentioning

confidence: 99%

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Fernandes-França¹,

Oliveira-Santos²,

Oliveira-Lira³

et al. 2019

Rev. colomb. cienc. pecu.

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Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.

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Section: Discussionmentioning

confidence: 99%

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Fernandes-França¹,

Oliveira-Santos²,

Oliveira-Lira³

et al. 2019

Rev. colomb. cienc. pecu.

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“…To overcome cryoinjuries occurred by vitrification procedures, many kinds of attempts have been applied directly or indirectly to the oocyte and embryo in many species ( Hwang et al, 2013 ; Chasombat et al, 2015 ; Hwang et al, 2016 ). In the present study, it was focused on the physical damages of the oocytes during vitrification procedures.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cytochalasin B Treatment on the Improvement of Survival Rate in Vitrified Pig Oocyte

et al. 2018

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To improve survival rates of vitrified pig oocytes, the treatment of cytoskeletal stabilizer on an appropriate time is one of the possible approaches. However, the exact treatment timing and effect of cytoskeletal stabilizer such as cytochalasin B (CB) is not well known during oocyte vitrification procedures. Thus, the present study was conducted to determine optimal treatment timing of CB during vitrification and warming procedures. In experiment 1, the survival rates of the post-warming pig oocytes were analyzed by fluorescein diacetate (FDA) assays with 4 classifications. In results, post-warming oocytes showed significantly (p<0.05) decreased number of alive oocytes (31.8% vs. 86.4%) compared to fresh control. In detail, the significant difference (p<0.05) was found only in strong fluorescence (18.2% vs. 70.5%) not in intermediate fluorescence groups (13.6% vs. 15.9%). In experiment 2, CB was treated before (CB-Vitri) and after (Vitri-CB) vitrification. In results, group of Vitri-CB showed significantly (p<0.05) higher (91.6%) survival rates compared to group of CB-Vitri (83.7%), significantly (p<0.05) and comparable with group of Vitri Control (88.7%) by morphological inspection. In FDA assay results, group of Vitri-CB showed significantly (p<0.05) higher (44.2%) survival rates compared to groups of CB-Vitri (36.7%) and Vitri Control (35.1%). In conclusion, the increased survival rates of post-warming pig oocyte treated with Vitri-CB method are firstly described here. The main finding of present study is that the CB treatment during recovery could be helpful to refresh the post-warming pig oocyte resulting its improved survival rates.

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“…Improvements of bovine oocyte cryopreservation have been made over the years, for example, using macromolecule polymers as CPAs to lessen toxicity [71] , using cryotop can lead to a better outcome of vitrified bovine GV and MII oocytes [72] , and using solid-surface vitrification reduces the ultrastructural injuries [73] . Docetaxel treatment before vitrification can significantly decrease injury to the cytoskeleton of bovine oocytes, thereby improving their post-warming survival rate and development potential [74] . Other chemicals, such as conjugated linoleic acid [75] , L-carnitine [76] , glutathione [77] and a cAMP agonist [78] have also improved outcomes.…”

Section: Cryopreservation Of Mammalian Oocytesmentioning

confidence: 99%

Cryopreservation of farm animal gametes and embryos: recent updates and progress

Huang¹,

Gao²,

Hou³

et al. 2019

Front. Agr. Sci. Eng.

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Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously refined over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been significant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen-thawed porcine sperm in pig production.

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Pretreatment of in vitro matured bovine oocytes with docetaxel before vitrification: Effects on cytoskeleton integrity and developmental ability after warming

Cited by 15 publications

References 48 publications

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Effect of Cytochalasin B Treatment on the Improvement of Survival Rate in Vitrified Pig Oocyte

Cryopreservation of farm animal gametes and embryos: recent updates and progress

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