Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation

Abstract

A simple cryopreservation method (desiccation) is examined on Corylus avellana L. callus in current study. The effect of abscisic acid (ABA) concentration, preculture length on Murashige and Skoog (MS) medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) on callus survival rate were investigated. After cryo-storage, the calli were heated at 40°C followed by cold tap water for one minute, then transferred to the recovery medium. Calli survival was assessed 6-8 weeks after exiting from LN. There was a signi cant difference between ABA concentrations and different culture periods on medium containing mentioned hormone in survival rate but no signi cant difference was observed between the storage periods in LN. In the present study, the highest survival rate (45/79%) was obtained in both the treatment of 20 days preculture on medium containing 2 mg l − 1 ABA following 120 minutes desiccation-one day storage in LN and 150 minutes desiccation-one month storage in LN. Key MessageDesiccation method with the help of ABA and sucrose was an effective method in the successful cryopreservation of Corylus avellana L. callus.