Pretreatment with alcoholic extract of shape Crataegus oxycantha (AEC) activates mitochondrial protection during isoproterenol – induced myocardial infarction in rats

Abstract

Crataegus oxycantha (hawthorn) is used in herbal and homeopathic medicine as a cardiotonic. The present study was done to investigate the effect of the alcoholic extract of Crataegus oxycantha (AEC) on mitochondrial function during experimentally induced myocardial infarction in rat. AEC was administered orally to male albino rats (150-200 g), at a dosage of 0.5 ml/100 g body weight/day, for 30 days. At the end of the experimental period, the animals were administered isoproterenol (85 mg/kg body weight, s.c) for 2 days at an interval of 24 h. After 48 h, the rats were anaesthetized and sacrificed. The hearts were homogenized for biochemical and electron microscopic analysis. AEC pretreatment maintained mitochondrial antioxidant status, prevented mitochondrial lipid peroxidative damage and decrease in Kreb's cycle enzymes induced by isoproterenol in rat heart.

Effect ofCrataegusUsage in Cardiovascular Disease Prevention: An Evidence-Based Approach

“…As mitochondria produce 95% of energy necessary for heart function, therapeutic agents that could influence mitochondrial dysfunction are of special importance. Alcoholic extract of Crataegus oxyacantha (AEC) pretreatment maintained mitochondrial antioxidant status and prevented mitochondrial lipid peroxidative damage and decrease in Krebs cycle enzymes induced by isoproterenol in rat heart [29]. Another research showed that Crataegus fruit extracts decreased the mitochondrial membrane potential by 1.2–4.4 mV measured with a tetraphenylphosphonium-selective electrode and H 2 O 2 production measured fluorometrically.…”

The genus Crataegus: chemical and pharmacological perspectives

“…The action of these compounds on the cardiovascular system has led to the development of leaf and flower extracts, which are widely used in Europe. Other chemical constituents includes vitamin C, saponins, tannins, cardiotonic amines (phenylethylamine, tyramine, isobutylamine, O-methoxy phenylethylamine, choline and acetylcholine), purine derivatives (adenosine, adenine, guanine, caffeic acid, amygdalin), triterpene acids ursolic acid (Verma et al, 2007 Jayalakshmi et al, 2006;Garjani et al, 2000).…”

“…These amines were responsible for in vitro activity of Crataegus extracts on the guinea pig papillary muscle (Wagner & Grevel, 1982) and it also raised intracellular calcium thus prolongs the action potential, which supports for its inotropic activity (Kocyildiz et al, 2006). In another study, alcoholic extract of Crataegus oxycantha (AEC) was found to maintain mitochondrial antioxidant status, decreased Kreb's cycle enzymes induced by isoproterenol in rat heart and prevented mitochondrial lipid peroxidative damage (Jayalakshmi et al, 2006). to ameliorate post-angioplasty restenosis and prevent balloon catheter-induced intimal hyperplasia (Furst et al, 2010).…”

Cardio Protective Effects of Hydroalcholic Citrus Aurantium Extract on Myocardial Infarction Induced by Isoproterenol in Male Rats

“…Superoxide dismutase, which catalysis the dismutation of two superoxide radicals to form hydrogen peroxide and molecular oxygen. Therefore, the H 2 O 2 created is deactivated by either catalase or the GSH redox system containing of reduced glutathione as the cofactor for GP X and glutathione reductase [32]. Citrus aurantium pre-co-treatment exhibited a substantial increase in the level of serum SOD and catalase.…”