2012
DOI: 10.1016/j.ejphar.2012.05.007
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Pretreatment with interleukin-6 small interfering RNA can improve the survival rate of polymicrobial cecal ligation and puncture mice by down regulating interleukin-6 production

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Cited by 16 publications
(10 citation statements)
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“…Interestingly, this region also coincides with the binding sites of transcription factors PU.1 and NFkB. PU.1 is known to act as both a repressor and activator of several cytokine genes [73] and has been proposed to be involved IL-6 expression [74]. Moreover, this IL-6 upstream region has been previously reported to have basic transcriptional activity in gene reporter luciferase assays [75].…”
Section: Resultsmentioning
confidence: 78%
“…Interestingly, this region also coincides with the binding sites of transcription factors PU.1 and NFkB. PU.1 is known to act as both a repressor and activator of several cytokine genes [73] and has been proposed to be involved IL-6 expression [74]. Moreover, this IL-6 upstream region has been previously reported to have basic transcriptional activity in gene reporter luciferase assays [75].…”
Section: Resultsmentioning
confidence: 78%
“…Subsequent experiments exhibited that 0.001 EU/ml of endotoxin did not enhance the production of IL-6 and TNF-α in rat GMC, although a higher concentration of endotoxin (50 EU/ml) could increase the production of IL-6 and TNF-α (Fig 3D), indicating the up-regulation of IL-6 and TNF-α in rat GMC is indeed induced by purified C5a. Additionally, since IL-6 has recently been reported to up-regulate or down-regulate the expression of C5aR [34, 35], further experiment was designed to determine whether the expression of C5aR on the GMC was changed. The result displayed that there was not significant change of C5aR expression on the GMC membrane after C5a-stimulated IL-6 release (Fig 4).…”
Section: Resultsmentioning
confidence: 99%
“…The results showed that C5a could markedly up-regulate the expression of IL-6 and TNF-α in the GMC, and C5aR knockdown could greatly reduce the levels of IL-6 and TNF-α in the GMC stimulated with C5a, indicating that C5a could elevate the synthesis of IL-6 and TNF-α in the GMC though C5aR. Since IL-6 has recently been proven to be an important regulator of the C5aR [34, 35], next experiment was designed to confirm whether the expression of C5aR was effected on the surface of GMC. The result displayed that there was not significant change of C5aR expression on the GMC membrane after C5a-induced IL-6 production.…”
Section: Discussionmentioning
confidence: 99%
“…Likewise, systemic (i.v., i.p.) pretreatment of septic mice with PEI/siRNA polyplexes for the targeting of IL‐6 and TNFα significantly increased survival rates . Intravenously injected polyplexes for Gadd45β gene knockdown attenuated ischemia‐induced apoptosis of cardiomyocytes .…”
Section: Polyethylenimines For the Delivery Of Small Rnas In Vivomentioning
confidence: 99%