Prevalence and Antibiogram of Extended Spectrum Beta-Lactamase Producing Escherichia Coli

Modi, Syamal; Shukla, Indu; Chander, Yogesh; R, Sms

doi:10.14260/jemds/543

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Lower resistance rates to ciprofloxacin, usually prescribed in uncomplicated UTI and other infections, have been reported in Nepal (45%) [ 6 ], in India (52.80%) [ 23 ] and in Iran (60%) [ 20 ]. It was showed that uncontrolled use of quinolones in the past years led to a growing resistance to these antibiotics particularly to ciprofloxacin worldwide [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin

Anago

Ayi-Fanou²,

Akpovi

et al. 2015

Ann Clin Microbiol Antimicrob

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BackgroundBeta lactams are the most commonly used group of antimicrobials worldwide.The presence of extended-spectrum lactamases (ESBL) affects significantly the treatment of infections due to multidrug resistant strains of gram-negative bacilli. The aim of this study was to characterize the beta-lactamase resistance genes in Escherichia coli isolated from nosocomial infections in Cotonou, Benin.MethodsEscherichia coli strains were isolated from various biological samples such as urine, pus, vaginal swab, sperm, blood, spinal fluid and catheter. Isolated bacteria were submitted to eleven usual antibiotics, using disc diffusion method according to NCCLS criteria, for resistance analysis. Beta-lactamase production was determined by an acidimetric method with benzylpenicillin. Microbiological characterization of ESBL enzymes was done by double disc synergy test and the resistance genes TEM and SHV were screened by specific PCR.ResultsESBL phenotype was detected in 29 isolates (35.5%). The most active antibiotic was imipenem (96.4% as susceptibility rate) followed by ceftriaxone (58.3%) and gentamicin (54.8%). High resistance rates were observed with amoxicillin (92.8%), ampicillin (94%) and trimethoprim/sulfamethoxazole (85.7%). The genotype TEM was predominant in ESBL and non ESBL isolates with respectively 72.4% and 80%. SHV-type beta-lactamase genes occurred in 24.1% ESBL strains and in 18.1% of non ESBL isolates.ConclusionThis study revealed the presence of ESBL producing Eschericiha coli in Cotonou. It demonstrated also high resistance rate to antibiotics commonly used for infections treatment. Continuous monitoring and judicious antibiotic usage are required.

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Section: Discussionmentioning

confidence: 99%

Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin

Anago

Ayi-Fanou²,

Akpovi

et al. 2015

Ann Clin Microbiol Antimicrob

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Frequency of E coli and its sensitivity to nitrofurantion in patients with urinary tract infection

Khan

Umam²,

Yousaf³

et al. 2021

JMS

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Background: Urinary tract infection (UTI) is one of the most common bacterial illnesses in adults and is one of the most common indications for antibiotics. This study is conducted to determine frequency of E-Coli and its sensitivity to nitrofurantoin in patients with urinary tract infection. Materials and Methods: The study was conducted at a tertiary care hospital of District Peshawar, i.e. Khyber Teaching Hospital (KTH), after reviewed and approved by the Committee for Ethical Review of Research involving Human Subjects of KTH. Non-random consecutive sampling technique was used for the collection of data and data collection was completed in 06 months’ time i.e. from 20th February to 19th August 2018. The survey was conducted as a descriptive cross-sectional study using open Epi calculator, keeping confidence interval of 95%, sample size calculated is 148. Urine samples were collected in sterile containers and was sent to laboratory for routine examination, culture and sensitivity. A colony 105 or more was considered E Coli growth. Nitrofurantoin was used to check the sensitivity for organisms as per operational definition. All the tests and examinations were carried out under the supervision of experienced medical specialist and microbiologist. RESULTS: In this study, 148 patients were studied. Average age was 41.86 years + 11.62SD. E coli was found in 91(61.5%) of patients out of total 148 patients. Among UTI patients 78(85.7%) were sensitive to Nitrofurantoin. CONCLUSION: High prevalence of drug-resistant urinary tract pathogens, particularly to Nitrofurantoin suggests cautious use of antibiotic therapy for the treatment of UTI

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Prevalence and Antibiogram of Extended Spectrum Beta-Lactamase Producing Escherichia Coli

Cited by 2 publications

References 12 publications

Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin

Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin

Frequency of E coli and its sensitivity to nitrofurantion in patients with urinary tract infection

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