Prevalence and Antibiogram of Pseudomonas aeruginosa Isolated from West African Mud Creeper (Tympanotonus fuscatus)

Abstract

The menace of opportunistic infections arising from food contamination can be tackled with knowledge of agents effective against the etiologic agents. A bacteriological examination was carried out on Tympanotonos fuscatus (West African Mud Creeper) sold at different markets in Port Harcourt, Nigeria with interest on Pseudomonas aeruginosa and their antibiogram using standard microbiological methods. Results showed that parboiled samples had the mean total heterotrophic bacterial counts between 26.33±11.02x106 and 85.67±3.79x106 cfu/g, total coliform counts, 6.0±1.73x106 and 13.0±7.21x106 cfu/g and total Pseudomonad counts, 0.00 and 0.67±1.16x102 cfu/g. Roasted samples had total heterotrophic bacterial counts between 40.0±1.73x106 and 45.75±3.86x106 cfu/g, total coliform counts, 10.0±7.211x106 and 13.0±9.539 x 106 cfu/g and total Pseudomonad counts, 6.67±4.619x102 and 20.75±22.824 x102 cfu/g. There was difference (p<0.05) in the total heterotrophic bacteria counts of parboiled samples from the different markets but none in coliform and Pseudomonads counts. No difference (p>0.05) was recorded in counts from the roasted samples. Thirty-two (32) Pseudomonas isolates were obtained from the markets occurring least in the parboiled 6 (18.75%) samples compared to roasted 26 (81.25%) samples. Mile3 market had the highest occurrence 3 isolates (50%) for parboiled samples whereas Mile1 market with 1 isolate (16.67%) was lowest. Results of Antibiogram revealed that Pseudomonas species had varying sensitivity to all the antibiotics tested and was most susceptible to Ofloxacin (90.6%) and most resistant to Nalidixic acid (84.4%). Although the counts obtained are within the upper limits (5x107cfu/gm) recommended by the International Commission on Microbiological Specifications for Foods (ICMSF), there is need to properly cook Tympanotonus fuscatus before consumption to reduce risks of opportunistic pathogens. Infections from Pseudomonas species may be managed with appropriate doses of ofloxacin.

The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region

“…73 P. aeruginosa has been isolated from foods such as the West African Mud Creeper sold in local markets. 74 The one prior study of airborne microbes in Mali found that up to 25% of the bacterial isolates detected were opportunistic human pathogens. 13 Many of the genera phylogenetically related to human pathogens, as well as two of the potential pathogens confirmed at the species level using PCR, exhibited abundance in the smallest particle size fractions (<2.5 μm), highlighting the potential for long-distance transport.…”

Phenotypic Characterization of Bacteria Isolated from the Recreational Sites of Two Rivers in Orashi Region, Rivers State, Nigeria

“…The isolates were identified based on microscopy and their cultural as well as biochemical attributes. The bacterial species were identified phenotypically by examining their cultural identities based standard microbiological procedures, as reported by Sampson et al [14] and Akani et al [15]. This involved spreading aliquots of the serially diluted samples over the surface of solid agar plates and incubated at 37ºC for 24-48 hours.…”

Molecular Characterization of Antibiotic Resistant Bacteria from Soil within Rivers State University, Nigeria

“…Garba et al (2012) found that Pseudomonas had a high sensitivity to Ofloxacin and Gentamicin while simultaneously having a high resistance to Amoxicilin/Clavulanic acid (Augmentin) in their investigation. P. aeruginosa resistance is a serious public health problem, especially because it is a leading source of nosocomial infections in hospitals [16]. The sensitivity pattern of E. coli and Salmonella spp to antibiotics in this study showed that isolates were mostly resistant to Cefixime, Ceftazidime and Cefuroxime with all isolates (100%) resistant to these antibiotics.…”

“…The sensitivity of Pseudomonas spp to gentamicin and Ofloxacin in the present study is in agreement with the results of antibiogram of Pseudomonas spp. from the study by Akani et al [16]. The resistance of Pseudomonas spp.…”