Prevalence and antibiotic resistance of Campylobacter coli isolated from broiler farms in the Marrakesh Safi region, Morocco

Abstract

Background and Aim: Campylobacteriosis is a common foodborne disease epidemiologically linked to the consumption of poultry products. However, other sources, such as raw or contaminated milk, contaminated water or ice, contact with infected livestock, and pets, are reported. This study aimed to evaluate the prevalence and resistance to microbial resistance of Campylobacter coliM in broiler farms in the region of Marrakesh Safi, Morocco. Materials and Methods: The study was conducted between May and December 2017 and involved 35 broiler farms. One hundred and five cloacal swabs were collected from the eight provinces in the region of Marrakesh Safi, Morocco. Bacteriology method NM ISO/TS 10272-3: 2013 was used to isolate and identify Campylobacter spp. Molecular identification (polymerase chain reaction) was used for confirmation. A disk diffusion method on Mueller-Hinton agar was used for susceptibility testing. Five antibiotic agents, including first-line drugs, were evaluated. Results: Among 105 samples, 71.4% (75/105) were positive for Campylobacter spp. test and 56% (42/75) of isolates belonged to the species coli. Susceptibility profiles showed that 95.2% of C. coli strains were resistant to ampicillin, 92.8% to erythromycin and tetracycline, 85.7% to ciprofloxacin, and 7.1% to gentamicin. Conclusion: This study underlines the need to strengthen implementation of specific control procedures to decrease contamination of poultry meat with Campylobacter spp. and to reduce the use of antibiotics in the poultry sector.

Antimicrobial Usage, Susceptibility Profiles, and Resistance Genes in Campylobacter Isolated from Cattle, Chicken, and Water Samples in Kajiado County, Kenya

“…This resistance rate is somewhat worrying in contrast to previous findings from the outskirts of Thika, a city in Central Kenya [ 12 ]. The finding is consistent with the study by Asmai et al [ 33 ] who also reported a high phenotypic Campylobacter resistance rate of 92.8% to erythromycin. Going by the findings of this study, macrolide (erythromycin) would no longer be considered as an alternative therapy in systemic campylobacter infections in man.…”

“…None of the Campylobacter isolates in this study were susceptible to ampicillin, translating into 100% “acquired” resistance. Previous studies in other African countries including Tanzania and Morocco have reported resistance rate to this antibiotic at 63% and 95.2%, respectively [ 11 , 33 ]. The high ampicillin-resistant phenotypes in this study might be due to the reported usage of β -lactams (including amoxicillin or a combination of procaine penicillin and dihydrostreptomycin sulphate or cloxacillin and ampicillin) among farmers in the treatment of bacterial infections such as mastitis in cattle.…”

Antimicrobial Resistance and Virulence Factors of Campylobacter coli Isolated from Chicken in Côte d’Ivoire

“…prevalence (62.50 %) %) in our study is much lower than those found in Algeria (98%) [19], Spain (88%), Portugal (82%), and Malta (96.3%) [20] and Morocco (71 %) [21]. Nevertheless, prevalence identified present study is higher than the prevalence reported in Sweden (13.2%), Finland (3.9%), and Denmark (19%) [20,21]. However, this finding is lower than previous prevalence (above 70 %) in chicken ceaca reported by Goualié et al [12] in same area.…”

Chemical Composition and Antibacterial Activities of Eight Plant Essential Oils from Morocco against Escherichia coli Strains Isolated from Different Turkey Organs

“…Filali et al [ 44 ] reported a 100% efficacity of colistin against E. coli strains isolated from chickens with colisepticaemia. Furthermore, other pathogenic bacteria isolated from poultry meat such as Salmonella [ 45 ], Listeria [ 46 ], and Campylobacter [ 47 ] have also developed antibiotic resistance towards one or more antibiotics. Chaiba et al [ 48 ] spotted Methicillin-resistant S. aureus isolated from poultry farmers and chicken slaughters which stresses even more the fact that poultry could be a reservoir for antibiotic resistant microbes that could easily infect humans especially those with direct contact like farmers.…”