Prevalence and Antibiotic Resistance Pattern of Escherichia coli Isolated from Raw Dairy Milk

Hasan, Abul; Reza, Ahsanur; Rume, Farzana Islam; Meher, Mirza Mienur; Parvej, Md. Shafiullah; Anower, Akm Mostafa

doi:10.24925/turjaf.v6i9.1108-1113.1788

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…Escherichia coli is a food pathogen often present in raw milk of animal origin. These finding agree with the reports of Coastard et al [36], Kuehn [37] and Hasan et al [38].…”

Section: Discussionsupporting

confidence: 93%

Genetic Characterization of Multiple Antibiotics Resistance Genes of Escherichia coli Strain from Cow Milk and Its Products Sold in Abuja, Nigeria

Okechukwu

Amuta

Gberikon

et al. 2020

JABB

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Background: Antibiotic resistance has remained a global concern. Food pathogens that carry resistance genes will cause public health threat irrespective of their pathogenicity, as this pool of resistance genes are disseminated via food chain. The antibiotic susceptibility of E. coli isolates to different antibiotics were investigated and resistance genes were genetically identified by multiplex Polymerase Chain Reaction (PCR). The study aimed to determine the phenotypic antibiotic resistance pattern of the E. coli isolates and characterize the antibiotics resistance genes in the E. coli isolates from the Cow milk and milk products. Results: The study showed that the E. coli isolates were Multidrug resistant (MDR) to several antibiotics with resistance pattern of four to ten combinations of antibiotics and the antibiotics resistance occurred mostly in three classes of antibiotics, namely: β-lactams, Amphenicol and Tetracycline. The antibiotics resistance genes expressed in the multidrug resistant E. coli isolates were tetA 22(78.6%), dfrA1 9(32.1%), blaCITM and blaSHV 3(10.7%), tetB and blaTEM 1(3.6%), qnr 0(0%) and aac (3)-iv 0(0%). Conclusion: The study showed that the cow milk and its products sold on the streets of Abuja are contaminated with resistant E. coli that can pose health risk to the consumers. Monitoring and screening for resistant food pathogens in food especially of animal origin is vital to mitigate the prevalence of antibiotics resistance.

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“…Escherichia coli is a food pathogen often present in raw milk of animal origin. These finding agree with the reports of Coastard et al [36], Kuehn [37] and Hasan et al [38].…”

Section: Discussionsupporting

confidence: 93%

Genetic Characterization of Multiple Antibiotics Resistance Genes of Escherichia coli Strain from Cow Milk and Its Products Sold in Abuja, Nigeria

Okechukwu

Amuta

Gberikon

et al. 2020

JABB

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“…**Significant differences at p=0.01. E. coli=Escherichia coli antibiotics [33][34][35][36]. In addition, an antibiotic sensitivity pattern was observed for E. coli isolates from meat and meat by-products, revealing a high rate of resistance against E, followed by CR, AMP, OT, AML, and S. However, none of the isolates was resistant to CIP.…”

Section: Discussionmentioning

confidence: 99%

Development of multidrug-resistant Escherichia coli in some Egyptian veterinary farms

et al. 2022

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Background and Aim: Food of animal origin is considered a major source of foodborne diseases. In this context, multidrug-resistant (MDR) Escherichia coli pose a serious hazard to public health due to the consumption of food contaminated with antibiotics that are used for the treatment of various bacterial infections in farm animals. Therefore, this study aimed to determine the effect of the excessive use of antibiotics on the development of MDR E. coli strains in Egyptian poultry, dairy, and meat farms. Materials and Methods: A total of 1225 samples were randomly collected from poultry, dairy, and meat products intended for human consumption in different governorates. E. coli were isolated from the collected samples and subjected to biochemical identification and antibiotic sensitivity tests with antibiotics commonly used in human and veterinary medicine. Then, amoxicillin (AML)- and oxytetracycline (OT)-resistant E. coli isolates were subjected to a polymerase chain reaction test to detect the blaTEM and tetA genes, respectively. Results: E. coli were isolated from 132 out of 350, 148 out of 350, 177 out of 350, and 35 out of 175 poultry, milk, meat, and human samples, respectively. Most of the isolates expressed multidrug resistance, and resistance genes (blaTEM and tetA) were detected in all the tested AML- and OT-resistant E. coli isolates. Conclusion: Foods of animal origin may represent a source of MDR E. coli, which can be a major threat to public health.

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“…Based on their virulence agents, different E. coli strains were named and classified. One of the most important food-borne infections that can be spread by milk and dairy products is E. coli ( Hasan et al ., 2018 ). According to Zeinhom and Abdel-Latef (2014 ), E. coli is linked to potentially fatal conditions such as thrombotic thrombocytopenic purpura, hemolytic uremic syndrome, and hemorrhagic colitis (HC).…”

Section: Introductionmentioning

confidence: 99%

Application of some inorganic metal oxide nanoparticles to control E. coli in raw milk

Nazem,

Shaala,

Awad

2024

Open Vet J

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Background: Nanoparticles are regarded as magical bullets because of their exclusive features. Recently, the usage of nanoparticles has progressed in almost all aspects of science and technology due to its ability of revolutionizing certain fields. In the field of food science and technology, the application of nanoparticles is being researched in many various areas thus provide the dairy industry a variety of new attitudes for developing the quality, prolong shelf life, ensure safety and healthiness of foods. Aim: This study aimed to focus on the application of some inorganic metal oxide nanoparticles (zinc oxide (ZnO), magnesium oxide (MgO), and calcium oxide (CaO)) to control E. coli in raw milk and ensure its safety. Methods: The antibacterial action of certain nanoparticles (ZnO, MgO, and CaO) with multiple concentrations (0.1, 0.05, 0.025, 0.0125, 0.006, and 0.003 mg/ml) was evaluated against E. coli strains in UHT milk samples. As well, storage temperature and storage period effects were studied. Results: The findings of the current research revealed that inorganic metal oxide nanoparticles had a significant antibacterial role against E. coli, at the following order; ZnO, MgO, and CaO, respectively. The antibacterial effect of inorganic metal oxide nanoparticles is more noticed at lower temperatures. Conclusion: Inorganic metal nanoparticles can be used in the food industry for the purpose of the control of E. coli, and extension of the shelf life of the dairy products.

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Prevalence and Antibiotic Resistance Pattern of Escherichia coli Isolated from Raw Dairy Milk

Cited by 3 publications

References 23 publications

Genetic Characterization of Multiple Antibiotics Resistance Genes of Escherichia coli Strain from Cow Milk and Its Products Sold in Abuja, Nigeria

Genetic Characterization of Multiple Antibiotics Resistance Genes of Escherichia coli Strain from Cow Milk and Its Products Sold in Abuja, Nigeria

Development of multidrug-resistant Escherichia coli in some Egyptian veterinary farms

Application of some inorganic metal oxide nanoparticles to control E. coli in raw milk

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