Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

Permatasari, Dian Ayu; ANGGRAENI, FAUZIAH; BUDIARTO, BUDIARTO; Meles, Dewa Ketut; Hamid, Iwan Sahrial; PUSPITASARI, YULIANA; Effendi, Mustofa Helmi; Khairullah, Aswin Rafif; Wardhana, Dhandy Koesoemo; Ugbo, Emmanuel Nnabuike

doi:10.13057/biodiv/d231011

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…The pathogen is said to cause infection not only in bovines but also in humans and aquatic animals ( 3 ), so the rise in prevalence is justifiable as the pathogen spreads at one health interface. The current study showed a high prevalence of S. agalactiae compared with the study by Permatasari et al, ( 14 ). A study from Bangladesh reported 62.5% Klebsiella sp.…”

Section: Discussioncontrasting

confidence: 50%

“…showed 5–19.5% resistance against the tetracycline group and up to 6.9% resistance against β-lactam. Similarly, up to 32% resistance against cefquinome, kanamycin, ceftiofur, polymyxin B, and tetracycline was found against Klebsiella spp ( 14 ). However, our findings reported otherwise by demonstrating the effective response of K. pneumoniae against penicillin, tetracycline, and amoxicillin.…”

Section: Discussionmentioning

confidence: 99%

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Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles

Manan

Aqib

Shahbaz³

et al. 2023

Front. Vet. Sci.

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Streptococcus agalactiae and Klebsiella pneumoniae are emerging as major milk-borne pathogens. Additionally, resistance to antibiotics of pathogens is of concern. Therefore, this study investigated the prevalence and drug resistance of S. agalactiae and K. pneumoniae in mastitis milk samples and assessed the antimicrobial potential of sodium alginate (G)-stabilized MgO nanoparticles (M) and antibiotics (tylosin [T] and ampicillin [A]) against both of these pathogens. A total of n = 200 milk samples from cattle were collected using purposive sampling, and standard microbiological approaches were adopted to isolate target bacteria. Parametric and non-parametric statistical tests were used to analyze the obtained data. Four preparations, GT (gel-stabilized tylosin), GA (gel-stabilized ampicillin), GTM (tylosin and MgO nanoparticles stabilized in gel), and GAM (ampicillin and MgO nanoparticles stabilized in gel), were evaluated against both bacteria through well diffusion and broth microdilution method. The analysis revealed that 45.24% (95/210) of the milk samples were positive for mastitis, of which 11.58% (11/95) were positive for S. agalactiae and 9.47% (9/95) were positive for K. pneumoniae. S. agalactiae had a significantly higher zone of inhibition (ZOI) than K. pneumoniae against penicillin, tetracycline, and amoxicillin, whereas the opposite was observed against imipenem and erythromycin. All gel (G)-based preparations showed an increase in the percentage of ZOI compared with antibiotics alone, with GTM presenting the highest of all, i.e., 59.09 and 56.25% ZOI compared with tylosin alone against S. agalactiae and K. pneumoniae, respectively. Similarly, in a broth microdilution assay, the lowest MIC was found for K. pneumoniae (9.766 ± 0.0 μg/mL) against GTM, followed by GT, GAM, and GA after incubation for 24 h. A similar response was noted for preparations against S. agalactiae but with a comparatively higher MIC. A significant reduction in MIC with respect to incubation time was found at 8 h and remained until at 20 h against both pathogens. The cytotoxicity of the MgO nanoparticles used in this study was significantly lower than that of the positive control. Overall, this study found that K. pneumoniae and S. agalactiae appeared higher in prevalence and antimicrobial resistance, and sodium alginate-based antibiotics and MgO nanoparticles were effective alternative approaches for tackling antimicrobial resistance.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles

Manan

Aqib

Shahbaz³

et al. 2023

Front. Vet. Sci.

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Susceptibility patterns of Escherichia coli and streptococcal isolates from bovine mastitis cases to antibiotics and selected South African plant extracts with known antibacterial activities

Akinboye,

Adeyemo,

Karzis

et al. 2024

South African Journal of Botany

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A recent update on the use of antimicrobials for animal health in Yogyakarta, Indonesia

Wijayant,

Rosetyadew,

Pratama

et al. 2023

Int J One Health

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Background and Aim: Animal antimicrobials must be studied to determine if they have the same types, norms of use, and resistance patterns as those used in humans. This study aimed to facilitate the analysis of data on the use of antimicrobials in veterinary medicine and to aid in developing a strategy to prevent the emergence of antimicrobial resistance (AMR) in humans and animals. Materials and Methods: Data were obtained through a survey of 101 veterinarians working in animal clinics and independent practice in five regencies of Yogyakarta province. Results: Both of the antibiotics, antiparasitic (78.6%) and, antiseptic and disinfectant (68.0%), and antifungal (31.1%) were the most frequently used types of antimicrobials, while antivirals were the least used (9.7%). The most often treated animals with antimicrobials are pets (37%), followed by large and small livestock (30%), poultry (15%), and exotic animals (14%). Of the respondents, 89% were aware of the factors contributing to AMR, but only 47% monitored developments and expanded their understanding of AMR. The most common antibiotic classes were penicillin (71), tetracyclines (50), sulfonamides (41), fluoroquinolones (31), and aminoglycosides (27). Conclusion: All antimicrobials used in the animal health sector are also used in human medicine, which requires special consideration. This cross-use of antibiotics was a crucial factor in determining the cause of the spread of AMR between humans and animals. Keywords: animal health, antimicrobial use, Yogyakarta.

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Prevalence and antimicrobial resistance in Streptococcus agalactiae isolated from raw milk in Pasuruan and Lumajang districts, East Java, Indonesia

Cited by 4 publications

References 27 publications

Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles

Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles

Susceptibility patterns of Escherichia coli and streptococcal isolates from bovine mastitis cases to antibiotics and selected South African plant extracts with known antibacterial activities

A recent update on the use of antimicrobials for animal health in Yogyakarta, Indonesia

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