Prevalence and Antimicrobial Resistance of Bacterial Foodborne Pathogens Isolated from Raw Bivalve Molluscs Subjected to Consumption in Poland during a Ten-Year Period

Lopatek, Magdalena; Wieczorek, Kinga; Osek, Jacek

doi:10.3390/foods11213521

Cited by 7 publications

(4 citation statements)

References 56 publications

(86 reference statements)

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“…All V. parahaemolyticus isolates had the toxR genes reported by some researchers [47,49]. However, a lower percentage was reported by some reports [51,52]. Meanwhile, [53] said higher results.…”

Section: Discussionmentioning

confidence: 83%

Assessment of the Bacteriological Quality of Some Fish Products

Elbarbary,

Abd El-aziz,

Maky

et al. 2024

Egyptian Journal of Veterinary Sciences

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F ISH products are considered a nutritionally significant component of the human diet because they contain macronutrients (proteins, fats, and ash) and micronutrients (vitamins and minerals). Accordingly, 200 different commercial fish product samples (25 each of canned solid tuna, canned chunks tuna, canned crumbled tuna, canned anchovy, canned mackerel, canned sardine, herring, and tilapia fillets) of different brands were collected from various local and high supermarkets in Aswan City during 2020-2021. The samples were subjected to microbial quality assessment, and PCR was carried out to detect some virulence genes of fish-borne pathogens to evaluate the control measures for food safety. The results presented that the highest bacterial loads (cfu/g) in the examined fish products were proven in fillets tilapia (1.3×104±0.32) and herring (1.0×104±1.2) while anchovy, mackerel, and crumbled tuna were the highest anaerobic count with means of 7.3×103±1.2, 7×103±1.6 and 6.7×103±1.4, respectively. The prevalence of P. aeruginosa occurs with an incidence of 29%. The lasB gene (50%) has been the most detected virulence gene, followed by exoS (40%). V. parahaemolyticus was present in 7.5%, and the regulator toxin genes (toxR and L.tdh) were found in 33.3% and 22.2%. Additionally, 20% of the samples had A. hydrophila, and the aerA and act genes were found in 60% of the isolates. In addition, C. perfringens was detected in 33% of examined products, and the virulence genes cpa and etx were present and discovered to be a proportion of 20% and 50%, respectively. The current study concluded that the examined fish products possess one or more food poisoning virulence genes indicating unsanitary handling, transport, processing, storage practices, and inappropriate environmental conditions.

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Section: Discussionmentioning

confidence: 83%

Assessment of the Bacteriological Quality of Some Fish Products

Elbarbary,

Abd El-aziz,

Maky

et al. 2024

Egyptian Journal of Veterinary Sciences

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“…Such isolates were identified both in food and food-processing environments. Other studies also identified strains resistant to clindamycin in other countries, although the percentages of such isolates were rather low ( 7 , 21 ). However, there are also reports of high resistance rates of L. monocytogenes isolates to this antimicrobial ( 11 , 15 , 29 ).…”

Section: Discussionmentioning

confidence: 98%

Antimicrobial resistance of Listeria monocytogenes serogroups IIa and IVb from food and food-production environments in Poland

Lachtara,

Wieczorek,

Osek

2023

Journal of Veterinary Research

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Introduction Listeria monocytogenes is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually susceptible to most antibacterial substances, but resistance to some of them has been recently observed. The present study introduces the evidence on the emergence of antibiotic resistance among L. monocytogenes strains isolated from food and food-production environments in Poland. Material and Methods A total of 283 L. monocytogenes isolates classified into serogroups IIa and IVb which had been recovered from food and food production environments were tested with 17 antimicrobials. These included those that are recommended for treatment of severe listeriosis cases in humans. A multiplex PCR was used to identify serogroups, and a microbroth dilution method was applied for the determination of antibiotic resistance among the isolates tested. Results Only 34 (12.0%) strains were susceptible to all the antimicrobials used in the study. The remaining 249 (88.0%) strains displayed different instances of resistance to the antimicrobials tested, from insusceptibility to one (112 strains; 39.6%) to resistance to four antibacterial substances (6 strains; 2.1%). Among them, there were 38 strains (13.4%) with multiresistance patterns. Conclusion Polish food and its processing environments may be a potential source of antimicrobial-resistant L. monocytogenes, which may pose a potential health risk to consumers in the country.

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“…domestic animals used in food production or produce [16]. Shellfish surveillance studies have provided insight into the prevalence of Salmonella in shellfish, along with the serovars commonly connected with contamination [15,[17][18][19]. However, little work has been focused on the lineages commonly associated with shellfish, their relevance to human illness or the potential routes of contamination.…”

Section: Introductionmentioning

confidence: 99%

Characterization of MLST-99 Salmonella Typhimurium and the monophasic variant I:4,[5],12:i:- isolated from Canadian Atlantic coast shellfish

Hodges,

Cooper,

Koziol

et al. 2024

Microbiology

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Salmonella enterica subsp. enterica Typhimurium and its monophasic variant I 1;4,[5],12:i:- (MVST) are responsible for thousands of reported cases of salmonellosis each year in Canada, and countries worldwide. We investigated S. Typhimurium and MVST isolates recovered from raw shellfish harvested in Atlantic Canada by the Canadian Food Inspection Agency (CFIA) over the past decade, to assess the potential impact of these isolates on human illness and to explore possible routes of shellfish contamination. Whole-genome sequence analysis was performed on 210 isolates of S. Typhimurium and MVST recovered from various food sources, including shellfish. The objective was to identify genetic markers linked to ST-99, a sequence type specifically associated with shellfish, which could explain their high prevalence in shellfish. We also investigated the genetic similarity amongst CFIA ST-99 isolates recovered in different years and geographical locations. Finally, the study aimed to enhance the molecular serotyping of ST-99 isolates, as they are serologically classified as MVST but are frequently misidentified as S. Typhimurium through sequence analysis. To ensure recovery of ST-99 from shellfish was not due to favourable growth kinetics, we measured the growth rates of these isolates relative to other Salmonella and determined that ST-99 did not have a faster growth rate and/or shorter lag phase than other Salmonella evaluated. The CFIA ST-99 isolates from shellfish were highly clonal, with up to 81 high-quality single nucleotide variants amongst isolates. ST-99 isolates both within the CFIA collection and those isolated globally carried numerous unique deletions, insertions and mutations in genes, including some considered important for virulence, such as gene deletions in the type VI secretion system. Interestingly, several of these genetic characteristics appear to be unique to North America. Most notably was a large genomic region showing a high prevalence in genomes from Canadian isolates compared to those from the USA. Although the functions of the majority of the proteins encoded within this region remain unknown, the genes umuC and umuD, known to be protective against UV light damage, were present. While this study did not specifically examine the effects of mutations and insertions, results indicate that these isolates may be adapted to survive in specific environments, such as ocean water, where wild birds and/or animals serve as the natural hosts. Our hypothesis is reinforced by a global phylogenetic analysis, which indicates that isolates obtained from North American shellfish and wild birds are infrequently connected to isolates from human sources. These findings suggest a distinct ecological niche for ST-99, potentially indicating their specialization and adaptation to non-human hosts and environments, such as oceanic habitats.

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Prevalence and Antimicrobial Resistance of Bacterial Foodborne Pathogens Isolated from Raw Bivalve Molluscs Subjected to Consumption in Poland during a Ten-Year Period

Cited by 7 publications

References 56 publications

Assessment of the Bacteriological Quality of Some Fish Products

Assessment of the Bacteriological Quality of Some Fish Products

Antimicrobial resistance of Listeria monocytogenes serogroups IIa and IVb from food and food-production environments in Poland

Characterization of MLST-99 Salmonella Typhimurium and the monophasic variant I:4,[5],12:i:- isolated from Canadian Atlantic coast shellfish

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