Prevalence and Antimicrobial Resistance Profiles of Foodborne Pathogens Isolated from Dairy Cattle and Poultry Manure Amended Farms in Northeastern Ohio, the United States

Hailu, Woinshet; Helmy, Yosra A.; Carney-Knisely, Geoffrey; Kauffman, Michael D.; Fraga, Dean; Rajashekara, Gireesh

doi:10.3390/antibiotics10121450

Cited by 44 publications

(38 citation statements)

References 123 publications

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“…In the study Hailu et al [ 178 ] the authors confirmed the 100% multi-resistance (MDR) of L. monocytogenes isolates obtained from dairy and poultry farms, manure, and soil in Ohio, USA to lincomycin (clindamycin), Rifamicines (ifampicin), cephalosporins (ceftriaxone, cefoxitin), Penem (meropenem), macrolides (azithromycin) and trimethoprim sulfamethoxazole, with 100% resistance for each antimicrobial class. A high resistsnce among the tested strains was also observed to Aminoglycosides- streptomycin (98.5%), Quinolones-nalidixic acid (95.5%), Fluoroquinolone—levofloxacin (91%) and penicillin- ampicillin (89.5%).…”

Section: Mechanisms Of Transfer Of Resistance Based On Examples Of Va...mentioning

confidence: 98%

“…A high resistsnce among the tested strains was also observed to Aminoglycosides- streptomycin (98.5%), Quinolones-nalidixic acid (95.5%), Fluoroquinolone—levofloxacin (91%) and penicillin- ampicillin (89.5%). As wrote Authors all L. monocytogenes strains isolated from poultry farms were resistant to kanamycine, nalidic acis and levofloxin, and more than 50% Listeria strains obtained from dairy farms were resistant to the cited antibiotics [ 178 ].…”

Section: Mechanisms Of Transfer Of Resistance Based On Examples Of Va...mentioning

confidence: 99%

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Antibiotic Resistance in Bacteria—A Review

et al. 2022

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Background: A global problem of multi-drug resistance (MDR) among bacteria is the cause of hundreds of thousands of deaths every year. In response to the significant increase of MDR bacteria, legislative measures have widely been taken to limit or eliminate the use of antibiotics, including in the form of feed additives for livestock, but also in metaphylaxis and its treatment, which was the subject of EU Regulation in 2019/6. Numerous studies have documented that bacteria use both phenotypis and gentic strategies enabling a natural defence against antibiotics and the induction of mechanisms in increasing resistance to the used antibacterial chemicals. The mechanisms presented in this review developed by the bacteria have a significant impact on reducing the ability to combat bacterial infections in humans and animals. Moreover, the high prevalence of multi-resistant strains in the environment and the ease of transmission of drug-resistance genes between the different bacterial species including commensal flora and pathogenic like foodborne pathoges (E. coli, Campylobacter spp., Enterococcus spp., Salmonella spp., Listeria spp., Staphylococcus spp.) favor the rapid spread of multi-resistance among bacteria in humans and animals. Given the global threat posed by the widespread phenomenon of multi-drug resistance among bacteria which are dangerous for humans and animals, the subject of this study is the presentation of the mechanisms of resistance in most frequent bacteria called as “foodborne pathoges” isolated from human and animals. In order to present the significance of the global problem related to multi-drug resistance among selected pathogens, especially those danger to humans, the publication also presents statistical data on the percentage range of occurrence of drug resistance among selected bacteria in various regions of the world. In addition to the phenotypic characteristics of pathogen resistance, this review also presents detailed information on the detection of drug resistance genes for specific groups of antibiotics. It should be emphasized that the manuscript also presents the results of own research i.e., Campylobacter spp., E. coli or Enetrococcus spp. This subject and the presentation of data on the risks of drug resistance among bacteria will contribute to initiating research in implementing the prevention of drug resistance and the development of alternatives for antimicrobials methods of controlling bacteria.

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Section: Mechanisms Of Transfer Of Resistance Based On Examples Of Va...mentioning

confidence: 98%

Section: Mechanisms Of Transfer Of Resistance Based On Examples Of Va...mentioning

confidence: 99%

Antibiotic Resistance in Bacteria—A Review

et al. 2022

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“…The S. aureus and E. coli resistant isolates were relatively few and scarce since they were originating from different farms. Molecular methods such as sequencing, and genotyping could reveal the genetic determinants of this resistance [59,60]. However, a further correlation between the phenotypical and the genotypical resistance as well as hierarchical cluster analysis it is doubtful if they could shed light on the origins of these genes, given the remoteness of most of these farms and the scarcity of the isolates.…”

Section: Discussionmentioning

confidence: 99%

Safety Issues Regarding the Detection of Antibiotics Residues, Microbial Indicators and Somatic Cell Counts in Ewes’ and Goats’ Milk Reared in Two Different Farming Systems

et al. 2022

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Milk samples of ewes’ and goats’ unprocessed milk were collected from milk tanks from various farms in Epirus, Greece and classified in two groups according to the type of farming. Analyses of the samples included microbial inhibition assays to detect the presence of antibiotic residues, isolation of Staphylococcus aureus and Escherichia coli strains as microbiological indicators for susceptibility to antimicrobial medicines, Somatic Cells and Coagulase Negative Staphylococci (CNS) counts. These findings were correlated with each other as well as to the stage of the lactation period and to the type of the farming practices. Monitoring of bulk tank milk for residues of antibiotics should be performed after heating of the milk, on a regular basis, and should include at least two different tests. The results point out that the type of farming affects the CNS counts as well as the prevalence of residues in the milk. Furthermore, the inverse correlation between CNS counts and prevalence of residues of antibiotics suggests a possible protective role of CNS. Resistance of the bacterial indicators to antibiotics was random and relatively rare, perhaps acquired in past due to misuse of antibiotics, turning the indicator microorganisms to reservoir of resistance.

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“…Currently, Campylobacter infections in humans are treated with macrolides and fluoroquinolones when necessary. However, Campylobacter ’s resistance to these groups of antibiotics has been reported ( 14 , 15 ), which poses a threat to the effectiveness of existing antibiotic therapies in both medical and veterinary practices ( 16 ). Previous reports showed that 80%, 46%, 8%, and 100% of the Campylobacter isolated from chickens were resistant to tetracycline, erythromycin, ciprofloxacin, and penicillin, respectively ( 8 , 15 , 17 ).…”

Section: Introductionmentioning

confidence: 99%