Prevalence and antimicrobial resistance profiles of Listeria monocytogenes in spontaneous abortions in humans

Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

“…In total, forty-six (7.5%) L. monocytogenes isolates were recovered from the 617 specimens tested in the present study, which is comparable with the results of the study conducted by Lotfollahi et al (2) . Four (7.5%), 2 (5.7%), 5 (14.2%), 3 (8.5%), and 0 (0%) L. monocytogenes isolates were obtained from the placental tissue, urine, vaginal swabs, rectal swabs, and blood, respectively.…”

“…L. monocytogenes infection in humans, listeriosis, is a serious illness that affects mostly immunosuppressed individuals, newborns, and the elderly, with symptoms ranging from septicemia, meningitis, encephalitis, abortions, to occasional death (1) . In recent years, many outbreaks of listeriosis have been implicated in the contamination of trading nutriments such as vegetables, milk, and meat foodstuffs (2) . Listeria monocytogenes detection from specimens based on selective enrichment media followed by biochemical studies is arduous and requires at least 5 days for a positive diagnosis.…”

“…The InlJ (or lmo2821) gene is responsible for passage of L. monocytogenes through the intestinal barrier and can be used for evaluating virulence of L. monocytogenes (5) . Detection of virulent genes is necessary as it decreases the time and labor required for diagnosis and will be useful in a large-scale investigation for detecting virulent strain of Listeria monocytogenes species includes a range of strains with varying virulence and pathogenicity, and while numerous L. monocytogenes strains are naturally virulent and capable of produce major illness and death, others are avirulent (2) . Key virulence-related proteins and the genes encoding them can be targeted to better evaluate virulent strains of L. monocytogenes (5) (6) .…”

“…Listeria monocytogenes detection from specimens based on selective enrichment media followed by biochemical studies is arduous and requires at least 5 days for a positive diagnosis. Polymerase chain reaction (PCR) is a rapid method with high sensitivity and specificity for specific deoxyribonucleic acid (DNA) sequences and permits direct detection of the pathogens (2) . A PCR employing amplification of the iap, prfA, hly, inl, and plcA gene sequences was recently reported for L. monocytogenes detection (1) .…”

Polymerase chain reaction (PCR4)-based detection of hly and plc-A genes in Listeria monocytogenes isolated from dairy and meat products in Iran

“…Also it has been shown that the main source of infections is ready-to-eat (RTE) foods with long shelf -life making L. monocytogenes one of the major causative agents of food-related deaths (O'Connor et al, 2010). Listeria species are able to survive many food manufacturing processes and with a recent increased consumption of RTE and heat to eat products, L. monocytogenes has emerged as a significant food borne pathogen, causing serious illness in infants, pregnant women, elderly and immunocompromised individuals (Liu et al, 2007;Lotfollahi et al, 2011 LLO like PI-PLC and PC-PLC (Ward et al, 2010). In Iran, there is scanty information about characteristics and virulence factors of L. monocytogenes which could be isolated from different food stuffs.…”