Prevalence and Antimicrobial Susceptibility of Listeria monocytogenes Isolated from Beef, Pork and Chicken Sold in Makurdi Metropolis

Adikwu, Peter; Umeh, E. U.; Azua, E. T.; Obande, Godwin Attah

doi:10.9734/bmrj/2016/25663

Cited by 11 publications

(11 citation statements)

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“…Results of this study revealed a high prevalence of L. monocytogenes in raw beef and chevon sold in Lafia. The prevalence rate of Listeria species observed in this study was lower than the 95.8% prevalence rate reported in vegetable salads in Zaria, Kaduna state [24] but higher than the 39.6% and 7.8% observed in Sokoto [26] and in Makurdi, Benue state [19]. The high L. monocytogenes contamination observed in the raw meat samples was in concordance with an earlier report where 14 out of the 15 Listeria species isolated were L. monocytogenes [27].…”

Section: Discussioncontrasting

confidence: 84%

“…Isolation of Listeria from the meat samples were based on the method described by Ndahi et al [21] and Adikwu et al [19] with some modifications. Aseptically, 10g of each sample was added to 90 ml Listeria Enrichment broth (Oxoid, Basingstoke, UK) containing Listeria Selective Enrichment Supplement.…”

Section: Isolation Of Listeria Spmentioning

confidence: 99%

“…Antibiotic susceptibility of the isolated L. monocytogenes was determined by the Kirby-Bauer disk diffusion method on Mueller-Hinton Agar [19,25]. The antibiotics used include erythromycin (15µg), streptomycin (10µg), co-trimoxazole (1.25/23.75µg), rifampicin (5µg), nalidixic acid (30µg), ciprofloxacin (5µg), ampicillin (10µg), gentamicin (10µg), chloramphenicol (30µg), sparfloxacin (5µg) and ofloxacin (5µg).…”

Section: Antimicrobial Susceptibility Testmentioning

confidence: 99%

“…There has been a dearth of information on the epidemiology of listeriosis in most African countries, including Nigeria [16] with only few reports, when compared to other developed regions like Europe and United States of America [17]. This is because the organism seems not to have been given as much attention as is required [18,19]. Listeriosis is considered a serious health problem due to its high mortality rate and severity of symptoms.…”

Section: Introductionmentioning

confidence: 99%

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Listerial contamination of raw beef and chevon in north-central Nigeria

Chuku¹,

Obande²,

Sani³

2020

IMC J Med Sci

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Background and objective: Listeria sp. is a ubiquitous and frequently isolated foodborne pathogen. The prevalence of Listeria sp in raw beef and chevon sold in Lafia Nigeria, as well as their antibiotic susceptibility profile was evaluated. Methods: A total 104 samples comprising of 52 raw beef and 52 chevon were obtained from street vendors (hawkers), Shinge abattoir, Lafia old market and Lafia Modern Market. Isolation of Listeria sp. was performed on Listeria Selective Agar, following enrichment in supplemented Listeria Selective Broth. Identification of Listeria sp. was carried out by cultural and biochemical methods. Antimicrobial susceptibility of isolated L. monocytogenes was performed by standard disk diffusion method. Chi-square test was used to determine association between contamination levels at p=0.05. Results: Seven types of Listeria sp. were isolated. L. monocytogenes and L. ivanovii were the most frequently isolated contaminants in all meat types and from all sample sources. L. monocytogenes was isolated with a frequency of 64.4% (67/104) in the meat samples. Beef samples had the highest listerial contamination with a frequency of 58.2% (78/134) compared to chevon which had a listerial frequency of 41.8% (56/134). Resistance of L. monocytogenes to streptomycin and sparfloxacin was 58.2% and 55.2% respectively. Resistance to ampicillin (34.3%) and gentamicin (20.9%) was also observed. Resistances to multiple antimicrobials were detected in 11 L. monocytogenes isolates. Conclusion: The study demonstrated that the raw meat sold in Lafia was contaminated with several Listeria sp. L. monocytogenes showed high rate of resistance to several antimicrobial agents used for the treatment of listerial infection. Appropriate regulation and monitoring of livestock rearing and meat retailing practices are advocated to safeguard the health of consumers. Ibrahim Med. Coll. J. 2019; 13(2): 1-8

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Section: Discussioncontrasting

confidence: 84%

Section: Isolation Of Listeria Spmentioning

confidence: 99%

Section: Antimicrobial Susceptibility Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Listerial contamination of raw beef and chevon in north-central Nigeria

Chuku¹,

Obande²,

Sani³

2020

IMC J Med Sci

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“…Peter, Umeh, Azua, and Obande () indicated that 16 isolates of L. monocytogenes from pork, beef, and chicken were susceptible to gentamycin, cotrimoxazol, erythromycin, and chloramphenicol, but were resistant to amoxicillin, augmentin, cloxacillin, and tetracycline. Abdollahzadeh et al.…”

Section: Prevalence Of Antibiotic Resistance Among Food Isolates Of Lmentioning

confidence: 99%

Emergence of Antibiotic Resistance in Listeria monocytogenes Isolated from Food Products: A Comprehensive Review

Olaimat

Al‐Holy

Shahbaz

et al. 2018

Comp Rev Food Sci Food Safe

188

120

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Listeria monocytogenes is an opportunistic pathogen that has been involved in several deadly illness outbreaks. Future outbreaks may be more difficult to manage because of the emergence of antibiotic resistance among L. monocytogenes strains isolated from food products. The present review summarizes the available evidence on the emergence of antibiotic resistance among L. monocytogenes strains isolated from food products and the possible ways this resistance has developed. Furthermore, the resistance of food L. monocytogenes isolates to antibiotics currently used in the treatment of human listeriosis such as penicillin, ampicillin, tetracycline, and gentamicin, has been documented. Acquisition of movable genetic elements is considered the major mechanism of antibiotic resistance development in L. monocytogenes. Efflux pumps have also been linked with resistance of L. monocytogenes to some antibiotics including fluoroquinolones. Some L. monocytogenes strains isolated from food products are intrinsically resistant to several antibiotics. However, factors in food processing chains and environments (from farm to table) including extensive or sub-inhibitory antibiotics use, horizontal gene transfer, exposure to environmental stresses, biofilm formation, and presence of persister cells play crucial roles in the development of antibiotic resistance by L. monocytogenes.

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