Prevalence and antimicrobial susceptibility of respiratory pathogens in patients with cystic fibrosis

Abstract

Respiratory infection is very common in patients suffering from cystic fibrosis (CF). However, the antimicrobial resistance rate of isolates from CF patients is not often documented. In this study, 279 respiratory specimens of 146 patients were prospectively collected from July to December 2006. Microbiological cultures and antimicrobial susceptibility tests of the most frequently isolated bacteria were performed. Sputum and oropharyngeal swabs were processed for culture. During the study period, 50% of the patients harbored Staphylococcus aureus, 35% Pseudomonas aeruginosa, 4.7% Haemophilus influenzae. Methicillin resistant S. aureus (MRSA) were detected in 8 (6%) patients; ESBL and MBL-producing P. aeruginosa were not identified in these patients. The detection of MRSA in CF patients confirms that antimicrobial resistance patterns should be always kept under surveillance. Moreover, hygiene regulations in CF clinics should prevent a further spread of resistant bacterial strains.

Phenotypic and Genetic Characterization of Carbapenemase and ESBLs Producing Gram-negative Bacteria (GNB) Isolated from Patients with Cystic Fibrosis in Tehran Tospitals

“…We isolated P. aeruginosa, K. zaenae, A. xylosoxidans, A. denitrificans, K. pneumoniae, S. maltophilia, E. hermannii, E. agglomerans, E. cloacae, E. kobei and C. koseri. As reported by Paixão et al, in 2010, P. aeruginosa as is the most frequent pathogen in CF patients [39]. We obtained the same result.…”

Hypermutable Pseudomonas aeruginosa in Cystic Fibrosis Patients from Two Brazilian Cities

“…Nevertheless, we found an MBL gene (bla SPM-1 ) in only one carbapenem-resistant CF isolate. Reports of MBL among CF P. aeruginosa are sparse but indicate a low prevalence of these carbapenemases (18,19). This supports the view that carbapenem resistance mechanisms in CF P. aeruginosa are not solely due to widespread metallo beta-lactamases.…”

Detection of Metallo-β-Lactamases and Klebsiella pneumonia Carbapenemases in Pseudomonas aeruginosa Isolates From Cystic Fibrosis Patients

“…Colonization by pathogenic bacteria, especially P. aeruginosa, in the respiratory tract occurs at a young age in patients with CF and represents a major health problem because it is considered a serious cause of mortality and morbidity. A previous study of the prevalence and antibacterial susceptibility of bacterial isolates from CF patients in Germany, the U.S., and South America reported that P. aeruginosa isolates were the most frequent pathogens of CF patients (9). The educational level of mothers of CF children and family awareness were reported to play important roles in the spread of infection with P. aeruginosa.…”