Prevalence and Antimicrobial Susceptibility Pattern of Klebsiella Pneumoniae Isolated from Various Clinical Specimens in a Tertiary Care Hospital in Bangladesh

Sonia, Suraiya Jahan; Afroz, Shadia; Rasheduzzaman, Md.; Uddin, Kazi Hafiz; Shamsuzzaman, SM

doi:10.3329/medtoday.v32i2.48821

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…Result of this study revealed that K. pneumoniae had higher resistance to ciprofloxacin (82%). In consistent with this finding, another study [30] observed that K. pneumoniae bacterial types are generally insensitive to ciprofloxacin (88%). High resistance rate to imipenem and ciprofloxacin in the present study might be due to uncontrolled consumption of these antibiotics in Iraq.…”

Section: Discussionsupporting

confidence: 70%

Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources

Amer¹,

Khudhair²,

Hussein³

2022

Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9

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Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative capsulated bacterium that causes a variety of human diseases. This study was to investigate the prevalence and antibiotic susceptibility pattern of K. pneumoniae isolated from diverse clinical samples, as well as to discover some of the bacteria's virulence components using a molecular approach. One hundred and eighty patients suffering from respiratory, urinary, wounds and burns infections were enrolled in this study and evaluated for the presence of K. pneumoniae infection via culturing, microscopical, motility and biochemical characteristics as well as API 20E and VITEK 2 systems. Molecular technology was used for detection the presence of K1 and K2 genes as well as the presence and sequencing 16S ribosomal ribonucleic acid (16S rRNA) gene. The results showed that a low prevalence of K. pneumoniae (27.8%) in Iraqi patients with higher positivity rate in sputum samples (42%) compared to other clinical samples.. According to the results of phylogenetic tree construction of 16S rRNA gene, we identified six mutated clinical isolates of K. pneumoniae that were deposited in GenBank under the accession numbers of MW642198, MW642199, MW642200, MW642201, MW642202 and MW642203. The mutated 16S rRNA gene stains exhibited a significantly higher resistance to all antibiotics types that used in this study.

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Section: Discussionsupporting

confidence: 70%

Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources

Amer¹,

Khudhair²,

Hussein³

2022

Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9

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“…The isolation rate of K. pneumoniae from total processed samples came out to be 5.24% (248/4724) and from 1336 growth positive samples the isolation rate of K. pneumoniae came out to be18.56% (248/1336). The prevalence correlates to great extent with the studies by Nirwati et al, Gill et al, Farkhanda et al, Sodhi et al, Sonia et al and Odari et al who reported prevalence to be 17.36%, 22.43%, 15.18%, 21.23%, 23.73% and 15.7% respectively [17][18][19][20][21][22]. However, Pyakurel et al reported prevalence 31.4% which is higher than the present study 23.…”

supporting

confidence: 74%

Antimicrobial susceptibility pattern and phenotypic detection of metallo-beta lactamases in K. pneumoniae isolates in a tertiary care hospital

Paray

Kaur

2022

Int J Res Med Sci

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Background: K. pneumoniae is the most important pathogen causing community as well as nosocomial infections. The study was conducted with the aim to study antimicrobial susceptibility pattern of K. pneumoniae isolates as well as to detect MBL in carbapenem resistant isolates.Methods: The study was conducted for a period of six months from October 2021 to March 2022. All isolates of K. pneumoniae were obtained from various clinical samples. Antimicrobial susceptibility testing was done by Vitek 2 Compact system. Metallo β-lactamase detection was done by imipenem-EDTA combined disk method.Results: Out of total 1336 growth positive isolates, 248 isolates were of K. pneumoniae. From total 248 isolates, 162 were obtained from male patients and 86 were obtained from female patients. Maximum number (97) of isolates was obtained from age group of 61-80 years. Maximum (30.24%) isolates of K. pneumoniae were from urine and minimum (4.43%) from body fluids. Isolates were highly resistant towards antimicrobials tested whereas moderate sensitivity was reported for ertapenem, imipenem, meropenem and gentamicin. Isolates were highly sensitive to colistin followed by amikacin and tigecycline. MBL production was observed in 84.5% carbapenem resistant isolates.Conclusions: This present study highlighted that multidrug resistant strains of K. pneumoniae are common in tertiary care hospitals. Unwarranted and unrestricted usage of antimicrobials is associated with growing emergence of resistance. Therefore, regular monitoring of carbapenem resistance is important for developing strategies to control infections caused by K. pneumoniae.

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“…Lev et al (2018) reported that the overall prevalence of K. pneumoniae in hospital-acquired infections in Moscow, Russia, was 16.66%, of which; 30% were from urine and 3% from blood samples. Out of 500 samples, 75 K. pneumoniae (23.73%) were isolated from urine (18.67%), blood (8%) and other samples in Bangladesh (Sonia et al, 2020). In Indonesia, 167 (17.36%) K. pneumoniae isolates were recovered from 962 clinical specimens, of which 12 (7.19%) were from urine and 18 (10.78) from blood (Nirwati et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Out of 500 samples, 75 K . pneumoniae (23.73%) were isolated from urine (18.67%), blood (8%) and other samples in Bangladesh (Sonia et al, 2020). In Indonesia, 167 (17.36%) K .…”

Section: Discussionmentioning

confidence: 99%

Biotyping, virulotyping and biofilm formation ability of ESBL-Klebsiella pneumoniae isolates from nosocomial infections

Ahmed

Ibrahim

Abdelhaliem

et al. 2022

Journal of Applied Microbiology

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The aim of this study was to investigate the frequency, molecular characterization, virulence genes, resistance genes and antimicrobial profile of nosocomial extended spectrum beta lactamase producing Klebsiella species. A total of 22 (12.2%) K. pneumoniae strains were isolated from 180 clinical samples collected from hospitalized patients in Egypt. K. pneumoniae biotypes were B1 (72.8%), B3 (13.6%) and B4 (13.6%). The isolates were classified for the capsular serotypes, 86.4% (20/22) were of K1 serotype, while only two isolates (13.64%) were of K2 serotype. Hypermucoviscous K. pneumoniae isolates accounted for 68.2%. Biofilm formation ability of K. pneumoniae was determined by microtitre plate method. The majority of the isolates (40.9%) were moderate biofilm producers, while 27.3% were strong biofilm producers. All K. pneumoniae strains were positive for fimH and traT genes, while magA was identified in only 63.6% of the isolates. The antibiotic susceptibility profile of the isolates (n = 22) was determined by the disc diffusion technique using 23 different antibiotics. Streptomycin and imipenem are the most effective antibiotics against 22 tested K. pneumoniae isolates with sensitivity rates of 63.64% and 54.54% respectively. All tested K. pneumoniae isolates showed high resistance to amoxicillin∕clavulanate (100%), cefuroxime (100%) and ceftazidime (95.45%). Extended spectrum beta lactamases (ESBL) production and the presence of ESBL‐related genes were tested in the isolates. All the isolates tested positive for blaVIM, NDM1 and blaTEM, while only 81.8 %tested positive for the blaSHV gene. Increasing antimicrobial resistance in K. pneumoniae causing nosocomial infections limits the use of antimicrobial agents for treatment. Furthermore, the spread of biofilm, multiple drug resistant and ESBL‐producing K. pneumoniae isolates is a public threat for hospitalized patients.

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Prevalence and Antimicrobial Susceptibility Pattern of Klebsiella Pneumoniae Isolated from Various Clinical Specimens in a Tertiary Care Hospital in Bangladesh

Cited by 4 publications

References 7 publications

Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources

Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources

Antimicrobial susceptibility pattern and phenotypic detection of metallo-beta lactamases in K. pneumoniae isolates in a tertiary care hospital

Biotyping, virulotyping and biofilm formation ability of ESBL-Klebsiella pneumoniae isolates from nosocomial infections

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