Prevalence and Antimicrobial Susceptibility Profiles of Microorganisms Associated with Lower Reproductive Tract Infections in Women from Southern Poland—Retrospective Laboratory-Based Study

Abstract

Objective: Female infections affecting the genital tract include sexually transmitted diseases, endogenous infections such as vulvovaginal candidiasis, bacterial vaginosis (BV) or aerobic vaginitis (AV) and healthcare-associated infections. The aim of the study was to analyze the etiological factors of the vaginal dysbacteriosis, and the antimicrobial susceptibility of the dominant bacterial and fungal infections in different age groups of outpatient women from the Silesian Region. Materials and methods: A retrospective laboratory-based multi-center study encompassed 4994 women of different ages in Silesian Voivodeship, in the south of Poland; patients who had vaginal swabs collected as per physicians’ orders during the period from 1 January 2017 until 30 June 2018 were included in the study. The inclusion criteria were: non-hospitalized female, aged ≤80, with suspected vulvovaginal candidiasis or bacterial vaginosis and clinical sings of infections. Results: Gram-positive cocci were the ones most often isolated: Enterococcus faecalis (29.2%) and Streptoccoccus agalactiae (13.1%), followed by bacilli from the Enterobacteriaceae group, including Escherichia coli (26.3%). The presence of Streptococcus agalactiae was confirmed in 13.1%, slightly more often in the 45–80 age group, and Gardnerella vaginalis in 6.4%, most often in women aged 15–24. The prevalence of yeast-like infections was 24.3%, Candida albicans accounted for 78.3%, whereas among C. non-albicans spp.—C. glabrata dominated (14.9%) followed by C. parapsilosis (3.8%). The highest resistance was observed only in Streptococcus agalactiae as the MLSB mechanism (Macrolide-lincosamide-streptogramin B) was identified in 38.6% of strains. The prevalence of vulvovaginal candidiasis was 24.3%, the highest in women aged 15–44. Conclusions: Drug resistance in studied vulvovaginitis was associated only with Streptococcus agalactiae. A high proportion of yeast-like aetiology was found, probably associated with recurrent infections. In the analyzed cases only the Amsel criteria and culture methods were used for diagnosis without preparations and microbiological Nugent criteria.

Prevalence and Antibiotic Resistance Profile of Bacterial Pathogens in Aerobic Vaginitis: A Retrospective Study in Italy

“…E. faecium were more resistant compared to E. faecalis . The data reported in this work are in line with other retrospective and non-retrospective studies [ 7 , 12 , 19 , 20 , 21 ]. Furthermore, the percentages of resistance for the most worrying pathogens identified in the paper ( E.coli, K. pneumoniae, E. faecium and E. faecalis ) are in line with the data collected in Italy and exposed in the AR-ISS 2019 report for most of the molecules tested in the study [ 22 ].…”

Healthcare Associated Deep Pelvic Tissue Infection and Other Infections of the Female Reproductive Tract in Ukraine

“…In present study, the predominant pathogens of healthcare associated deep pelvic tissue infection and other infections of the female reproductive tract were E.coli, Enterobacter spp., K. pneumoniae, S.aureus, E.faecalis, P.aeruginosa. Our results was coherent with reports other studies [7,14,15 ].…”

Molecular characterization of prevalence, serotypes, virulence factors, and antibiotic resistance of Streptococcus agalactiae in a maternity hospital, Iran

“…Some isolates exhibited resistance to non-beta-lactam antibiotics, including clindamycin, erythromycin, and fluoroquinolones [3]. The susceptibility to penicillin and trimethoprim/sulfamethoxazole of S. agalactiae was reported while the strains isolated from patients aged 45-54 were resistant to clindamycin and erythromycin [44]. Penicillin and ceftriaxone have been reported to exert cidal effects on GBS isolates suggesting penicillin as the first choice for antibiotic prophylaxis and treatment of GBS infections [12].…”