Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander

Gómez‐Aldapa, Carlos A.; Segovia-Cruz, Jesús A.; Cerna-Cortés, Jorge F.; Rangel‐Vargas, Esmeralda; Salas-Rangel, Laura P.; Gutiérrez‐Alcántara, Eduardo J.; Castro-Rosas, Javier

doi:10.1016/j.fm.2016.05.014

Cited by 30 publications

(21 citation statements)

References 30 publications

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“…In contrast to the present study, Pagadala et al (2015) found that 5.4% ( n = 259) of tomato was positive for generic E. coli in Maryland, Delware and New Jersey of United States. In the city of Pachuca, Hidalgo state of Mexico, Gomez-Aldapa et al (2016) reported that 47% ( n = 100) of coriander samples were contaminated with generic E. coli and the concentrations ranged between <3 and 1100 MPN/g. In Aizawl city of India, Chellapandi et al (2015) observed that 4.88% ( n = 27) of fresh vegetables (tomato, potato and cabbage) contained E. coli .…”

Section: Resultsmentioning

confidence: 99%

A study on prevalence of microbial contamination on the surface of raw salad vegetables

Mritunjay

Kumar

2017

3 Biotech

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The present work evaluates the microbiological quality of raw salad vegetables (RSV) consumed in Dhanbad city, India. A total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. E. coli O157:H7, Listeria monocytogenes and Salmonella sp. were detected by real-time polymerase chain reaction (qPCR) subsequent to isolation. Results showed that all the samples were found positive for total coliform; however, E. coli was detected in 16.7% of the total samples. Pathogenic microorganisms such as E. coli O157:H7, L. monocytogenes and Salmonella spp. were detected in 1.3, 3.5 and 4.0%, respectively, of the total samples. However, pathogens were not detected in any of the cabbage samples. The Exiguobacterium sp. (Strain ISM SP 2014) was detected in the spinach sample while studying the bacterial contamination, reported for the first time on the surface of RSV. The 16S rRNA gene sequencing showed less than 92% similarity with sequences available in the public domain.

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Section: Resultsmentioning

confidence: 99%

A study on prevalence of microbial contamination on the surface of raw salad vegetables

Mritunjay

Kumar

2017

3 Biotech

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“…Asimismo, su presencia en muestras de origen animal también ha sido reportada, incluyendo Lima y otras regiones peruanas (20,21) . La presencia de microorganismos resistentes a los antimicrobianos en los alimentos humanos es un problema creciente en América Latina (22) .…”

Section: Discussionunclassified

Presencia de Enterobacteriaceae y Escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en Lima

Ruiz-Roldán

Martínez-Puchol

Gomes

et al. 2018

Rev Peru Med Exp Salud Publica

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Objetivos. El objetivo del presente estudio fue describir la presencia de Enterobacteriaceae en muestras de carne recolectadas en mercados tradicionales de Lima y establecer los niveles de resistencia a antimicrobianos y la presencia de betalactamasas de espectro extendido (BLEE) y AmpC en Escherichia coli. Materiales y métodos. Se recolectaron un total de 138 muestras de carne, 64 (46,4 %) de pollo, 44 (31,9 %) de carne de res y 30 (21,7%) de carne de cerdo. Las bacterias aisladas pertenecieron a 17 géneros diferentes, y específicamente 14 fueron clasificados como Enterobacteriaceae. Se analizó la sensibilidad frente a diez agentes antimicrobianos mediante el método de difusión de disco Kirby-Bauer, se determinó la presencia de BLEE y AmpC mediante las pruebas de doble disco y de inducción de imipenem-ceftazidima, respectivamente. Resultados. Los niveles de resistencia a los antimicrobianos fueron altos frente a trimetoprima-sulfametoxazol, ampicilina, tetraciclina, ácido nalidíxico, ciprofloxacino y cloranfenicol. Existen diferencias significativas en los niveles de resistencia a los antibióticos según el tipo de carne (pollo, carne de res y cerdo) (p <0,05). Los niveles de resistencia a múltiples antimicrobianos (MDR) fueron particularmente altos en pollo y cerdo (98,2 % y 86,4 %, respectivamente). Además, la presencia de BLEE en Escherichia coli aisladas de carne de pollo fue del 59,4 %. Conclusiones. Los niveles de resistencia a los antimicrobianos fueron altos frente a los antibióticos usados frecuentemente en humanos, se destaca el pollo y la res como potenciales reservorios de Escherichia coli productoras de BLEE y pAmpC, respectivamente.

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“…EPEC strains were EPNW7 and EPNC6 isolated from raw nopalitos ( Opuntia ficus‐indica L . [Gómez‐Aldapa, Cerna‐Cortes, et al, ]), and EPCSA225 isolated from a ready‐to‐eat cooked vegetable salad (Bautista‐De León, Gómez‐Aldapa, Rangel‐Vargas, Vázquez‐Barrios, & Castro‐Rosas, ). EAEC strain was kindly donated by Dr. J.F.…”

Section: Methodsmentioning

confidence: 99%

Behavior of thirteen foodborne bacteria on whole Hass avocado and potential of roselle calyx extracts as alternative disinfectant agents of avocado

Gómez‐Aldapa

Torres-Viela

Amaya-Acosta

et al. 2017

Journal of Food Safety

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The behavior of thirteen foodborne bacteria onto whole Hass avocado and the antibacterial effect of Hibiscus sabdariffa calyx extracts, sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on contaminated avocado were investigated. Avocados were inoculated with foodborne bacteria: Listeria monocytogenes, Shigella flexneri, Staphylococcus aureus, Salmonella Typhimurium, S. Typhi, S. Montevideo, Escherichia coli O157:H7, non‐O157:H7‐Shiga toxin‐producing E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, enteroinvasive E. coli, enteroaggregative E. coli, and Vibrio cholerae O1. The antibacterial effect of four roselle calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, colloidal silver, and acetic acid against all foodborne bacteria were examined onto avocados. All foodborne bacteria successfully attached and survived onto avocado at least 20 days. The acetonic and methanolic roselle extracts caused a greater reduction (until 4‐log10 in some cases) in the concentration of all foodborne bacteria than sodium hypochlorite, colloidal silver, and acetic acid on contaminated avocados. Practical applications Hass avocado have been associated with foodborne disease caused by Salmonella. The sodium hypochlorite and colloidal silver solution are widely used to disinfect fruit and vegetables in restaurants and home in Mexico. Even, the Health Secretariat of Mexico promotes their use as disinfectants of fruit and vegetables. However, a number of studies have shown that chemical agents used to eliminate pathogenic bacteria on fruits have a limited antimicrobial effect. Roselle calyx extracts are reported to have an antimicrobial effect. This study provides information on the significant impact of acetone and methanol roselle calyx extracts may be a potentially useful addition to disinfection procedures of avocado in the field and processing plants.

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Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander

Cited by 30 publications

References 30 publications

A study on prevalence of microbial contamination on the surface of raw salad vegetables

A study on prevalence of microbial contamination on the surface of raw salad vegetables

Presencia de Enterobacteriaceae y Escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en Lima

Behavior of thirteen foodborne bacteria on whole Hass avocado and potential of roselle calyx extracts as alternative disinfectant agents of avocado

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