Prevalence and characteristics of a novel bovine adenovirus type 3 with a natural deletion fiber gene

Shen, Yan; Liu, Jie; Zhang, Yinghui; Ma, Xiaoyu; Yue, Hua; Tang, Cheng

doi:10.1016/j.meegid.2020.104348

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…Our observations were consistent with the previous reports showing the prevalence of BoHV-1 and BVDV in China was ∼40 and 50.10%, respectively (11,33). As reported previously, the infection rate of BPIV3 and BRSV is relatively lower (34). However, a survey in Slovenia showed that the most prevalent virus was BRSV instead, followed by BCoV, BPIV3, and BVDV, while BoHV-1 was less frequently detected (35).…”

Section: Discussionsupporting

confidence: 92%

Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex

et al. 2022

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Bovine respiratory disease complex (BRDC) occurs widely in cattle farms. The main viral pathogens include bovine viral diarrhea virus (BVDV), Bovine herpesvirus 1 (BoHV-1), bovine parainfluenza virus type 3 (BPIV3), and bovine respiratory syncytial virus (BRSV), and the newly emerged influenza D virus (IDV). In this study, we have developed a one-step multiplex real-time Polymerase Chain Reaction (PCR) capable of simultaneously detecting these five viral pathogens causing BRDC. The established assay could specifically detect targeted viruses without cross-reaction with others. The detection limit was ~10 copies/reaction for single real-time PCR and 100 copies/ reaction for multiplex real-time PCR assay. A total of 213 nasal samples from cattle with signs of respiratory tract disease were then collected for performance evaluation of the established platform, proving that the method has good specificity and sensitivity. The surveillance data suggested that BVDV and BoHV-1 infections are the dominant cause of BRDC in the herd, whereas the detection rate of IDV, BIPV3, and BRSV is relatively lower. In summary, the established assay provides technical support for rapid clinical detection of BRDC associated viral pathogens to guide the formulation of BRDC prevention and control measures.

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Section: Discussionsupporting

confidence: 92%

Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex

et al. 2022

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“…The previous study has found that some adenovirus isolates had partial gene deletion (Marinheiro et al, 2011;Su et al, 2011;Pan Q. et al, 2018;Shen et al, 2020). For example, both Marinheiro et al (2011) and Su et al (2011) reported HAdV-7 variants in which E3 regions were partially deleted.…”

Section: Comparative Genomic Analysis Of the Four Hadv Isolatesmentioning

confidence: 98%

Development and Application of a Fast Method to Acquire the Accurate Whole-Genome Sequences of Human Adenoviruses

Zhao

Guan

et al. 2021

Front. Microbiol.

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The whole-genome sequencing (WGS) of human adenoviruses (HAdVs) plays an important role in identifying, typing, and mutation analysis of HAdVs. Nowadays, three generations of sequencing have been developed. The accuracy of first-generation sequencing is up to 99.99%, whereas this technology relies on PCR and is time consuming; the next-generation sequencing (NGS) is expensive and not cost effective for determining a few special samples; and the third-generation sequencing technology has a higher error rate. In this study, first, we developed an efficient HAdV genomic DNA extraction method. Using the complete genomic DNA instead of the PCR amplicons as the direct sequencing template and a set of walking primers, we developed the HAdV WGS method based on first-generation sequencing. The HAdV whole genomes were effectively sequenced by a set of one-way sequencing primers designed, which reduced the sequencing time and cost. More importantly, high sequence accuracy is guaranteed. Four HAdV strains (GZ01, GZ02, HK35, and HK91) were isolated from children with acute respiratory diseases (ARDs), and the complete genomes were sequenced using this method. The accurate sequences of the whole inverted terminal repeats (ITRs) at both ends of the HAdV genomes were also acquired. The genome sequence of human adenovirus type 14 (HAdV-B14) strain GZ01 acquired by this method is identical to the sequence released in GenBank, which indicates that this novel sequencing method has high accuracy. The comparative genomic analysis identified that strain GZ02 isolated in September 2010 had the identical genomic sequence with the HAdV-B14 strain GZ01 (October 2010). Therefore, strain GZ02 is the first HAdV-B14 isolate emergent in China (September 2010; GenBank acc no. MW692349). The WGS of HAdV-C2 strain HK91 and HAdV-E4 strain HK35 isolated from children with acute respiratory disease in Hong Kong were also determined by this sequencing method. In conclusion, this WGS method is fast, accurate, and universal for common human adenovirus species B, C, and E. The sequencing strategy may also be applied to the WGS of the other DNA viruses.

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“…Analysis of the intact fiber of the isolate revealed 79 amino acid deletions in the shaft domain and 74 unique amino acid mutations compared with the available complete sequences for fiber genes in the GenBank database ( Figure 1 ). Furthermore, it was confirmed that BAdV-3 with the natural deletion partial fiber gene has a wide geographical distribution in China ( 14 ). Considering that fiber is the major structural component of the adenovirus capsid that interacts with the cellular receptor and determines host cell tropism, and that deletions and mutations in this region have previously been reported to affect the pathogenicity of adenovirus ( 20 , 21 ), further studies of this isolate are warranted.…”

Section: Introductionmentioning

confidence: 92%

“…The pathological changes in BAdV-3 infected cattle were mainly confined to the lungs, characterized by inflammatory cell infiltration, necrotizing bronchitis, type II alveolar cell proliferation, and alveolar septa thickening (8)(9)(10)(11). Since Darbyshire et al isolated BAdV-3 from the conjunctiva of healthy dairy cows in the UK in 1965, it has been detected in many countries, geographically including Asia, Europe, North America, South America and Africa (4,7,(12)(13)(14)(15). Serological detection showed positive rates of BAdV-3 antibodies in cattle in Belgium, Finland, Iran, Poland and Turkey ranging from 46.7 to 92.3% (5,(16)(17)(18)(19), indicating that BAdV-3 has been widely prevalent in the world.…”

mentioning

confidence: 99%

Pathogenicity of a novel bovine adenovirus type 3 with a natural deletion partial fiber gene in BALB/c mice

Zou

et al. 2023

Front. Vet. Sci.

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ObjectiveA novel Bovine adenovirus type 3 (BAdV-3) with a natural deletion partial fiber gene was isolated in 2020 and named BO/YB24/17/CH. The objective of this study was to understand the pathogenicity of this virus.MethodsThiry-two 3-week-old BALB/c mice were divided into three experimental groups and a control group. Mice in the experimental groups were intranasally inoculated with virus, and mice in the control group were inoculated with MDBK cell supernatant. Mice were weighed and clinically examined daily for appearance of any clinical signs. Three infected mice and one control mouse were euthanized at 1, 3, 5, 7, 9, 11, 13, and 15 days after inoculation. Tissue samples were collected for histopathological examination, immunohistochemical staining, and detection of the replication dynamics of virus.ResultsAll infected mice developed mild clinical signs such as lethargy, weight loss, loss of appetite, and a rough hair coat, and gross lesions were observed as pulmonary punctate hemorrhage, lobular atrophy and splenomegaly. Histopathological examination revealed thickening of alveolar septa and mildly dilated splenic nodules and blurred red-white medullary demarcation in the spleen. Immunohistochemical results further confirmed that the production of the above lesions was due to viral infection. Importantly, unlike previously reported BAdV-3 detection only in the lungs and trachea, this isolate could be detected in multiple organs such as the heart, liver, spleen, kidney, and even blood by virus isolation and titration and real-time PCR methods.Clinical significanceThis study provides further insight into the pathogenicity of the fiber region deletion strain BO/YB24/17/CH in BALB/c mice, which provides a reference for the prevention and control of BAdV-3 as well as the development of vaccines.

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Prevalence and characteristics of a novel bovine adenovirus type 3 with a natural deletion fiber gene

Cited by 7 publications

References 33 publications

Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex

Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex

Development and Application of a Fast Method to Acquire the Accurate Whole-Genome Sequences of Human Adenoviruses

Pathogenicity of a novel bovine adenovirus type 3 with a natural deletion partial fiber gene in BALB/c mice

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