Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada

Abstract

This study was conducted to estimate the prevalence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) in retail chicken meat and broiler chickens from the Province of Quebec, Canada, and to characterize LA-MRSA isolates. A total of 309 chicken drumsticks and thighs were randomly selected in 2013 from 43 retail stores in the Monteregie. In addition, nasal swabs and caeca samples were collected in 2013-2014 from 200 broiler chickens of 38 different flocks. LA-MRSA was not detected in broiler chickens. Fifteen LA-MRSA isolates were recovered from four (1.3%) of the 309 chicken meat samples. Multi-Locus Sequence Typing (MLST) and SCCmec typing revealed two profiles (ST398-MRSA-V and ST8-MRSA-IVa), which were distinct using pulse-field gel electrophoresis (PFGE) and microarray (antimicrobial resistance and virulence genes) analyses. In addition to beta-lactam resistance, tetracycline and spectinomycin resistance was detected in all isolates from the 3 positive samples of the ST398 profile. Southern blot hybridization revealed that the resistance genes aad(D) and lnu(A), encoding resistances to aminoglycosides and lincosamides respectively, were located on plasmid. All isolates were able to produce biofilms, but biofilm production was not correlated with hld gene expression. Our results show the presence of two separate lineages of MRSA in retail chicken meat in Quebec, one of which is likely of human origin.

Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

“…Multi-drug resistance (MDR) was defined as resistance to three or more families of antibiotics. Further comparison of MDR in MRSA and MSSA isolates, MDR was identified in all MRSA isolates and the proportion of multi-drug resistant strains in MRSA was almost three times higher than that of MSSA, which was in line with a previous study [ 31 ]. All these findings indicate the severity of antimicrobial resistance of MRSA isolates, which may be attributed to the increase in affinity of MRSA in acquiring mobile genetic elements (MGEs), such as transposons or conjugative plasmids carrying antimicrobial resistance genes [ 28 , 30 ].…”

Hazard assessment of Staphylococcus with positive coagulase in meat produced and distributed in the Northern regions of Cameroon

“…On the other hand, the resistance profiles of the SPC samples were higher for the Penicillin G, the Oxytetracycline, and The Oxacillin in the butcheries. Some similar results were also found in the beef and commercialized avians in Quebec [29] and Lumbumbashi [30]. In the slaughterhouses, the resistance to Staphylococcus might have originated from animals slaughtered, but in the distribution of meat, the importance of the probable transmissions of resisting germs between people and meat proves that the peak of resistance obtained could be the consequence of a frequent usage of antibiotic molecules for the treatment of patients at some hospitals of Cameroon [31].…”

Antimicrobial Resistance and Genetic Lineages of Staphylococcus aureus from Wild Rodents: First Report of mecC-Positive Methicillin-Resistant S. aureus (MRSA) in Portugal

“…Later, MRSA was found in individuals in human communities (community-associated (CA)-MRSA) who have not had previous contact with health facilities [5]. More recently, livestock-associated MRSA (LA-MRSA) has also been widely reported among several species of animals including pigs, poultry and cows [6][7][8]. Moreover, it seems that most mammals can be colonized and/or infected by MRSA since it has also been isolated from pets, such as dogs, cats and horses, and several species of free-living animals [9,10].…”