Prevalence and characteristics of Salmonella isolates recovered from retail raw chickens in Shaanxi Province, China

Li, Yilan; Yang, Qingzhen; Cao, Chenyang; Cui, Shenghui; Yang, Hua; Xiao, Yingping; Yang, Baowei

doi:10.1016/j.psj.2020.07.038

Cited by 29 publications

(13 citation statements)

References 46 publications

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“…A comparison of prevalence findings from different studies should be assessed with caution, as variability in sample collection methods, location, time, and isolation protocols may affect results. Nevertheless, our results here are consistent with previous findings of poultry products being more frequently contaminated with Salmonella than other meat types ( Jørgensen et al, 2002 ; Hyeon et al, 2011 ; Kim et al, 2012 ; Li et al, 2020 ) due to the high-frequency colonization and carriage of Salmonella in the microbiome of healthy poultry animals ( Antunes et al, 2016 ). The highest recovery of Salmonella in our study was in ground turkey (14/240, 5.83%) followed by chicken (23/478, 4.81%) samples, likely due to ground poultry counterparts requiring additional processing steps, which increase opportunities for cross-contamination.…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Profiles of Non-typhoidal Salmonella From Retail Meat Products in California, 2018

et al. 2022

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Non-typhoidal Salmonella remains a leading cause of foodborne illness in the United States, with food animal products serving as a key conduit for transmission. The emergence of antimicrobial resistance (AMR) poses an additional public health concern warranting better understanding of its epidemiology. In this study, 958 retail meat samples collected from January to December 2018 in California were tested for Salmonella. From multivariable logistic regression, there was a 6.47 (90% CI 2.29–18.27), 3.81 (90% CI 1.29–11.27), and 3.12 (90% CI 1.03–9.45) higher odds of contamination in samples purchased in the fall, spring, and summer than in winter months, respectively, and a 3.70 (90% CI 1.05–13.07) higher odds in ground turkey compared to pork samples. Fourteen distinct serotypes and 17 multilocus sequence types were identified among the 43 isolates recovered, with S. Kentucky (25.58%), S. Reading (18.60%), S. Infantis (11.63%), and S. Typhimurium (9.30%) comprising the top serotypes. High prevalence of resistance was observed in retail chicken isolates for streptomycin (12/23, 52.17%) and tetracycline (12/23, 52.17%), in ground turkey isolates for ampicillin (8/15, 53.34%), and in ground beef isolates for nalidixic acid (2/3, 66.67%). Fourteen (32.56%) were susceptible to all antimicrobials tested, 11 (25.58%) were resistant to one drug, and 12 (27.91%) were resistant to two drugs. The remaining six isolates (13.95%) were multidrug-resistant (MDR, ≥3 drug classes) S. Infantis (n = 4), S. Reading (n = 1), and S. Kentucky (n = 1). Whole-genome sequencing (WGS) identified 16 AMR genes and 17 plasmid replicons, including blaCTX–M–65 encoding ceftriaxone resistance and a D87Y mutation in gyrA conferring resistance to nalidixic acid and reduced susceptibility to ciprofloxacin. The IncFIB(pN55391) replicon previously identified in connection to the worldwide dissemination of pESI-like mega plasmid carriage in an emerged S. Infantis clone was detected in four of the six MDR isolates. Genotypes from WGS showed high concordance with phenotype with overall sensitivity and specificity of 95.31% and 100%, respectively. This study provides insight into the AMR profiles of a diversity of Salmonella serotypes isolated from retail meat products in California and highlights the value of routine retail food surveillance for the detection and characterization of AMR in foodborne pathogens.

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Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Profiles of Non-typhoidal Salmonella From Retail Meat Products in California, 2018

et al. 2022

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“…Nevertheless, 70% of multidrug resistant was exhibited by S. enterica isolates from beef cattle in Mexico (Delgado‐Suárez et al., 2019 ). Although the number of MDR S. enterica is relatively low in this study compared to other literature (Li et al., 2018 ; Siriken et al., 2015 ), it is still a public health concern as MDR Salmonella serovars are considered to be more virulent than non‐MDR Salmonella (Dong et al., 2014 ). The most frequently detected resistance was to Tetracycline (28.9%), followed by Streptomycin (22.2%), Sulfisoxazole (20.0%), and Ampicillin (17.8%).…”

Section: Discussionmentioning

confidence: 50%

Withdrawn: Investigation on Salmonella enterica, Escherichia coli, and coliforms in beef from Ethiopian abattoirs: A potential risk of meat safety

Zelalem

Abegaz

Kebede

et al. 2022

Food Science & Nutrition

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The aims of this study were to determine the prevalence of Salmonella enterica (S. enterica) antimicrobial resistance based upon phenotypic assessment, and level of sanitation indicator organisms from 150 beef carcasses collected from three representative abattoirs in eastern, central, and southern Ethiopia. Samples were screened for S. enterica prevalence following the U.S. Department of Agriculture Microbiology Laboratory Guidebook (MLG) and confirmed by real‐time PCR. The S. enterica isolates were phenotypically evaluated for susceptibility to a panel of 13 antimicrobials using disk diffusion method as described in Clinical and Laboratory Standard Institutes guidelines. In addition, the coliform, total coliform, and generic E. coli populations were quantified by plating onto E. coli/coliform petrifilms. A total of 45 isolates of S. enterica were confirmed using real‐time PCR. The overall prevalence of S. enterica at the carcass level was 22.7% (95% CI, 16.0–30.0). However, based on the sampling points, S. enterica prevalence detected on hides was 13.3% (95% CI, 8.0–18.7), pre‐evisceration was 12.0% (95% CI, 7.3–17.3), and post‐evisceration was 4.7% (95% CI, 1.3–8.0). The prevalence of S. enterica was significantly lower on post‐evisceration as compared to hides (p = .009) and pre‐evisceration (p = .022). No differences were detected for the prevalence of S. enterica between abattoirs (p = .346). Finally, the prevalence of S. enterica was highest during the wet season (p = .011). The overall mean log CFU/cm2 ± SD of generic E. coli, coliform, and total coliform counts were 4.55 ± 0.99, 4.91 ± 1.13, and 4.98 ± 1.09, respectively. About 20% of S. enterica exhibited phenotypic multidrug resistant. The most frequently detected resistance was to Tetracycline (28.9%), followed by Streptomycin (22.2%) and Sulfisoxazole (20.0%). The data from this study highlight the need to implement interventions on improvement of sanitary practices in abattoirs.

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“…S . Infantis was the most prevalent serotype in this study, thus adding additional evidence to multiple reports that have described this serovar as a global emerging pathogen in poultry production in the Americas ( Valderrama et al, 2014 ; Cunha-Neto et al, 2018 ; Gymoese et al, 2019 ; Lapierre et al, 2020 ; Mejía et al, 2020 ), Europe ( EFSA, European Food Safety Authority; European Centre for Disease Prevention and Control ECDC, 2019 ), and Asia ( Ishihara et al, 2020 ; Li et al, 2020 ).…”

Section: Discussionsupporting

confidence: 75%

Investigating the dynamics of Salmonella contamination in integrated poultry companies using a whole genome sequencing approach

et al. 2022

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The study of non-typhoid Salmonella in broiler integrations has been limited by the resolution of typing techniques. Although serotyping of Salmonella isolates is used as a traditional approach, it is not of enough resolution to clearly understand the dynamics of this pathogen within poultry companies. The aim of this research was to investigate the epidemiology and population dynamics of Salmonella serotypes in 2 poultry integrations using a whole genome sequencing approach. Two hundred and forty-three Salmonella isolates recovered from the broiler production chain of 2 integrated poultry companies were whole genome sequenced and analyzed with dedicated databases and bioinformatic software. The analyses of sequences revealed that S . Infantis was the most frequent serotype (82.3%). Most isolates showed a potential for resistance against medically important antibiotics and disinfectants. Furthermore, 97.5% of isolates harbored the pESI-like mega plasmid, that plays an important role in the global dissemination of AMR. SNP tree analysis showed that there were clones that are niche-specific while other ones were distributed throughout the broiler production chains. In this study, we demonstrated the potential of whole genome sequencing analysis for a comprehensive understanding of Salmonella distribution in integrated poultry companies. Data obtained with these techniques allow determination of the presence of genetic factors that play an important role in the environmental fitness and pathogenicity of Salmonella .

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Prevalence and characteristics of Salmonella isolates recovered from retail raw chickens in Shaanxi Province, China

Cited by 29 publications

References 46 publications

Antimicrobial Resistance Profiles of Non-typhoidal Salmonella From Retail Meat Products in California, 2018

Antimicrobial Resistance Profiles of Non-typhoidal Salmonella From Retail Meat Products in California, 2018

Withdrawn: Investigation on Salmonella enterica, Escherichia coli, and coliforms in beef from Ethiopian abattoirs: A potential risk of meat safety

Investigating the dynamics of Salmonella contamination in integrated poultry companies using a whole genome sequencing approach

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