Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt

Amer, Said; Zidan, Sherif; Adamu, Haileeyesus; Ye, Jianbin; Roellig, Dawn M.; Xiao, Lihua; Feng, Yaoyu

doi:10.1016/j.exppara.2013.09.002

Cited by 67 publications

(51 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of dairy cattle in industrialized nations have shown a dominance of C. parvum, especially its IIa subtypes in pre-weaned calves (Xiao, 2010;Amer et al 2013). Subtype IIaA15G2R1 is especially common and is overwhelmingly the dominant subtype in most areas (Xiao, 2010).…”

Section: R Y P T O S P O R I D I U M S P E C I E S I N a N I M A L Smentioning

confidence: 99%

“…In developing countries C. bovis is the dominant species in pre-weaned calves, in addition to C. parvum, C. ryanae and C. andersoni (Silverlås et al 2010;Meireles et al 2011;Muhid et al 2011;Wang et al 2011a;Budu-Amoako et al 2012b;Venu et al 2012;Amer et al 2013;Silva et al 2013;Silverlås and Blanco-Penedo, 2013;Zhang et al 2013a). A small number of these studies subtyped C. parvum (Amer et al 2010;Imre et al 2011;Meireles et al 2011;Muhid et al 2011;Wang et al 2011a;Silva et al 2013), with C. parvum IId subtypes identified as the dominant C. parvum in China and Malaysia (Muhid et al 2011;Wang et al 2011a;Zhang et al 2013a) and both IIa and IId dominant in Egypt (Amer et al 2010;.…”

Section: R Y P T O S P O R I D I U M S P E C I E S I N a N I M A L Smentioning

confidence: 99%

See 1 more Smart Citation

Cryptosporidiumspecies in humans and animals: current understanding and research needs

2014

Self Cite

View full text Add to dashboard Cite

S U M M A R YCryptosporidium is increasingly recognized as one of the major causes of moderate to severe diarrhoea in developing countries. With treatment options limited, control relies on knowledge of the biology and transmission of the members of the genus responsible for disease. Currently, 26 species are recognized as valid on the basis of morphological, biological and molecular data. Of the nearly 20 Cryptosporidium species and genotypes that have been reported in humans, Cryptosporidium hominis and Cryptosporidium parvum are responsible for the majority of infections. Livestock, particularly cattle, are one of the most important reservoirs of zoonotic infections. Domesticated and wild animals can each be infected with several Cryptosporidium species or genotypes that have only a narrow host range and therefore have no major public health significance. Recent advances in next-generation sequencing techniques will significantly improve our understanding of the taxonomy and transmission of Cryptosporidium species, and the investigation of outbreaks and monitoring of emerging and virulent subtypes. Important research gaps remain including a lack of subtyping tools for many Cryptosporidium species of public and veterinary health importance, and poor understanding of the genetic determinants of host specificity of Cryptosporidium species and impact of climate change on the transmission of Cryptosporidium.

show abstract

Section: R Y P T O S P O R I D I U M S P E C I E S I N a N I M A L Smentioning

confidence: 99%

Section: R Y P T O S P O R I D I U M S P E C I E S I N a N I M A L Smentioning

confidence: 99%

Cryptosporidiumspecies in humans and animals: current understanding and research needs

2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, it was found that C. parvum was the dominant species in buffaloes and cattle in Ismailia province (65.7%) (Helmy et al, 2015). While, Amer et al (2013a) found that the prevailing occurrence of C. ryanae and the subtype family IId of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. The PCR analysis of the gp60 gene was successful for seven C. parvum positive specimens as well as two specimens that were negative in SSU rRNA PCR.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

View full text Add to dashboard Cite

Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.

show abstract

“…All 26 C. bovis isolates had 100 % homogeneity with each other at the SSU rRNA locus and were identical to bovine samples in Brazil (EF493331; Thomaz et al, 2007), the USA (EU203217; Feltus et al, 2008), Iran (AB441689; Keshavarz et al, 2009), India (GQ345005; Khan et al, 2010), Poland (KC618594; Rzeżutka & Kaupke, 2013) and Egypt (AB777172; Amer et al, 2013). C. bovis type I (CBType I) was the only type found in this study and in Henan Province (Wang et al, 2011b).…”

Section: Distribution and Percentage Of Cryptosporidium Speciesmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Fang

Wang

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt

Cited by 67 publications

References 60 publications

Cryptosporidiumspecies in humans and animals: current understanding and research needs

Cryptosporidiumspecies in humans and animals: current understanding and research needs

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Contact Info

Product

Resources

About