Prevalence and characterization of Escherichia coli O157 and O157:H7 in retail fresh raw meat in South China

Zhang, Shuhong; Zhu, Xuemei; Wu, Qingping; Zhang, Jumei; Xu, Xiaoke; Li, Haigang

doi:10.1007/s13213-015-1037-x

Cited by 24 publications

(25 citation statements)

References 36 publications

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“…Goncuoglu et al (2010) [29] showed that prevalence of resistance of E. coli O157:H7 isolated from meat in Turkey against cephalothin, streptomycin, sulfamethoxazole and sulfonamides were 36.36%, 9.09%, 14.28% and 7.14%, respectively. Zhang et al (2015) [27] reported that E. coli O157:H7 isolates of raw meat in China were highly resistant to penicillin (100%), chloramphenicol (64.29%) and ampicillin (57.14%) which was similar to our findings. [10] reported that the most commonly detected antibiotic resistance genes in the raw beef meat samples were blaSHV (70.14%), aac(3)-IV (64.17%), tetA (58.20%), aadA1 (49.25%), CITM (46.26%) and dfrA1 (43.28%) which was similar to our findings.…”

Section: Discussionsupporting

confidence: 90%

“…They showed that the prevalence of O157 serogroup in the meat samples of beef, sheep, goat and camel were 31.34%, 28.57%, 36% and 25%, respectively. Previous study which was conducted in China [27] showed that of 551 samples studied, 21 (3.81%) were positive for E. coli O157 and seven (1.27%) for O157:H7. They showed that the highest prevalence rate was found in beef (13.32%), pork (6.90%), chicken (3.28%) and duck (2.54%).…”

Section: Discussionmentioning

confidence: 88%

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Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Mashak¹

2018

JFNR

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Escherichia coli O157:H7 is one of the most dangerous zoonotic pathogens of meat. The present investigation was done to study the prevalence and antibiotic resistance of E. coli O157:H7 strains isolated from raw meat samples. A total of 458 meat samples were collected. Samples were cultured using what? After which sorbitol negative isolates were analyzed for rfbO157 and flicH7 genes. Thirty-six out of 458 meat samples were positive for sorbitol negative strains of E. coli (7.86%). All of the sorbitol negative strains were also positive for rfbO157 and flicH7 genes. Prevalence of E. coli O157:H7 strains were 7.86%. The prevalence of E. coli O157:H7 in chicken meat was higher than the other samples (16.25%). The genes that encode resistance to ampicillin (CITM) (100%), gentamicin (aac(3)-IV) (94.44%) and tetracycline (tetA) (61.11%) had the highest prevalence. Escherichia coli O157:H7 strains from raw meat samples from ruminants and poultry had the highest resistance to ampicillin (100%), tetracycline (83.33%) and gentamicin (83.33%) respectively. Strains of antibiotic resistant E. coli 0157:H7 found in this present study are of public health importance.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 88%

Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Mashak¹

2018

JFNR

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“…Molecular typing is useful to determine the genetic relationship of pathogens isolated from different sources. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC PCR) is a simple, cost effective tool with high discriminatory power for genotyping of different bacterial pathogens [11][12][13]. In order to investigate the occurrence of STEC in Zagazig City, Egypt, minced beef and chicken meat purchased from food outlets and human samples from outpatient clinics at Sharkia Governorate were examined.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Ahmed

MacLeod²,

Bayomi³

et al. 2017

Zagazig Veterinary Journal

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A total of 550 meat samples (300 minced beef and 250 chicken meat) marketed in Zagazig City, Sharkia Governorate, Egypt, as well as 150 human stool samples were examined for Shiga toxin producing E. coli. Results revealed that the isolation rates of E. coli O157:H7 versus non-O157 were 1.7% versus 2.3% in minced beef, 0.8% versus 2% in chicken meat and 0.7% versus 2.7% in human stools. Other identified serotypes were including O111:H8 (25%), O26:H11 (20.8%), O55:H7 (16.7%) and O113:H21 (4.2%). Virulence associated genes were identified in E. coli serotypes, stx1 and stx2 were characterized in 16.7% and 62.5% of the isolates, while, eaeA and hlyA genes were identified in 50% and 70.8% of the examined serotypes, respectively. Genotyping of E. coli O157:H7 serotype from different sources using Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) fingerprinting revealed heterogenicity of the isolates, however, human and minced beef isolates were grouped in the same cluster indicating potential transmission of infection from contaminated beef to human consumers. In conclusion, ERIC-PCR is a highly discriminatory, reliable and cost-effective tool for tracing sources of infection with bacteria. Public health education and application of strict hygienic measures during slaughtering, transportation and preparation of meat are essential to minimize the risk of contamination and transmission of infection to consumers.

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“…Reference strain of E. coli O157:H7 ATCC 35150 was included as positive controls, and distilled water was used as a negative control. Amplified products were separated by performing electrophoresis using 1.2% agarose gel with GoldView stain (0.005%, v/v) [11].…”

Section: Methodsmentioning

confidence: 99%

“…For example, some E. coli O157 strains that were resistant to penicillin were found in China [11]. Ciprofloxacin-and ampicillin-resistant isolates were also ubiquitous [2,12].…”

mentioning

confidence: 99%

Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections

Xie

Dong²,

Zhu³

et al. 2017

Urol Int

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Background:Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. Objectives: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E. coli O157 based on the prevalence of urinary tract infection (UTI) in Hubei, China. Results: We obtained 23 (8.07%) E. coli O157 isolates from 285 UTI patients in Hubei, China. All isolates were subjected to antibiotic susceptibility analysis, and molecular typing was performed using ERIC-PCR and MLST. Antimicrobial susceptibility results indicated that most strains were resistant to penicillin (95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All isolates were discovered to be multiresistant (resistance to more than 3 antibiotics). Genetic variability analysis showed that all of the isolates were grouped into 4 clusters both by ERIC-PCR and MLST. Conclusion: Our findings demonstrated the presence of E. coli O157 in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of E. coli O157 risk assessment in UTIs.

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Prevalence and characterization of Escherichia coli O157 and O157:H7 in retail fresh raw meat in South China

Cited by 24 publications

References 36 publications

Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections

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