Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Major Hospitals in Gabon

Dikoumba, Annicet-Clotaire; Onanga, Richard; Boundenga, Larson; Bignoumba, Michelle; Ngoungou, Edgard-Brice; Godreuil, Sylvain

doi:10.1089/mdr.2020.0497

Cited by 6 publications

(7 citation statements)

References 52 publications

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“…Among all kinds of bacterial resistance, the CTX-M extended spectrum beta-lactamase (ESBL) is the most dominant beta-lactam enzyme, and it is found mostly among the members of Enterobacteriaceae (1). In recent years, the CTX-M gene has been reported in different countries (2)(3)(4)(5). The CTX-M β-lactamase family can be divided into five groups based on their amino acid identities (6): the CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, and CTX-M-25 groups.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

et al. 2022

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CTX-M extended spectrum beta-lactamase-producing Escherichia coli cause severe health hazards in livestock breeding. To date, little is known about antibiotic resistance differences among bacterial isolates from yaks, cows, and beef cattle; therefore, the aims of this study were to analyse the prevalence of CTX-M-producing E. coli in yak, beef cattle, and dairy cattle feces from different provinces in China. A total of 790 fecal samples from yaks, beef cattle, and dairy cows were used. Among all the samples, 523 non duplicate E. coli isolates were identified, and 29.6% of samples harbored CTX-M producers. The results showed that these E. coli strains harbored 15 clusters of CTX-M genes: CTX-M-79, CTX-M-55, CTX-M-15, CTX-M-14, CTX-M-28, CTX-M-179, CTX-M-65, CTX-M-24, CTX-M-27, CTX-M-102, CTX-M-105, CTX-M-173, CTX-M-238, CTX-M-196, and CTX-M-10. The dominant resistance genes were CTX-M-15, CTX-M-14, and CTX-M-55. Moreover, the distribution of CTX-M genes was related to geographical region. Based on the above findings, we reasoned that bovines are potential reservoirs of antibiotic resistance, and this problem should be given adequate attention.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

et al. 2022

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“…For example, production of TEM-type ESBL in E. coli was widespread in China, followed by that of SHV and CTX-M. However, in Canada, SHV-type is widespread, followed by those of TEM and CTX-M. To date, CTX-M has been reported to be the most widely distributed ESBL-producing gene in India (Dikoumba et al, 2021). The results of the present study revealed that TEM (79.17%) is the most predominant ESBL gene in Chinese retail food samples, which is consistent with the results of another study (Yang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with other classes of drugs, β‐lactam antibiotics are relatively inexpensive, safe, and have fewer side effects, making them the most used antibiotics in human medicine. ESBL‐producing bacteria are resistant to penicillin and new‐generation cephalosporins (Dikoumba et al, 2021 ). Therefore, it is necessary to monitor antibiotic‐resistant phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Extended‐spectrum β‐lactamase‐producing Enterobacteriaceae from ready‐to‐eat foods: Genetic diversity and antibiotic susceptibility

Liu,

Chen,

Luifu

et al. 2023

Food Science & Nutrition

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Ready‐to‐eat (RTE) foods are widely marketed in China and are important components of everyday diet. In this study, a total of 2000 RTE food samples were analyzed, 252 (12.60%) of which were positive for Enterobacteriaceae, and 48 were identified as containing extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia coli isolates. Furthermore, the antimicrobial resistance patterns of these isolates to 14 antimicrobial agents revealed that most isolates were resistant to aminoglycosides and β‐lactam antibiotics. The TEM‐type gene was prevalent in our isolates (79.17%). The isolates (n = 48) were classified into three clusters based on the ERIC‐PCR results. Forty‐eight sequence types were found without duplicates, revealing genetic variation and relatedness among isolates. Thus, the results demonstrated the presence of ESBL‐producing Enterobacteriaceae in Chinese RTE foods. The results of this study provide insights into the spread of antibiotic‐resistant strains and improve understanding of microbial risks.

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“…It detects only ESBL enzymes from the CTX-M-1 and M-9 groups. On the other hand, CTX-M enzymes are the most common ESBL enzymes in K. pneumoniae , and the strains with this resistance mechanism are isolated with the highest frequency, not only in Poland, but also worldwide [ 13 , 14 , 15 , 16 , 17 ]. Moreover, the evaluated assay does not detect all beta-lactamases genes, like ampC-like enzymes.…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

Sękowska

Bogiel

2022

Antibiotics

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Increasing antimicrobial resistance of Gram-negative rods is an important diagnostic, clinical and epidemiological problem of modern medicine. Therefore, it is important to detect multi-drug resistant strains as early on as possible. This study aimed to evaluate Eazyplex® SuperBug CRE assay usefulness for beta-lactamase gene detection among Gram-negative rods, directly from urine samples and positive blood cultures. The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture.

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Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Major Hospitals in Gabon

Cited by 6 publications

References 52 publications

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

Extended‐spectrum β‐lactamase‐producing Enterobacteriaceae from ready‐to‐eat foods: Genetic diversity and antibiotic susceptibility

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

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