Prevalence and characterization of extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in ready-to-eat vegetables

Kim, Hong-Seok; Chon, Jung‐Whan; Kim, Youngji; Kim, Dong‐Hyeon; Kim, Mu-sang; Seo, Kun‐Ho

doi:10.1016/j.ijfoodmicro.2015.04.049

Cited by 84 publications

(63 citation statements)

References 35 publications

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“…Similar results were observed in vegetables collected in South Korea (Kim et al, 2015), salads in the Netherlands (Reuland et al, 2014), and Spain (Egea et al, 2011), displaying a new route of introduction for ESBLs and pathogenic Enterobacteriaceae.…”

Section: Expansion Toward New Ecological Nichessupporting

confidence: 74%

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

2016

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β-lactamases are the primary cause of resistance to β-lactams among members of the family Enterobacteriaceae. SHV enzymes have emerged in Enterobacteriaceae causing infections in health care in the last decades of the Twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. Likely originated from a chromosomal penicillinase of Klebsiella pneumoniae, SHV β-lactamases currently encompass a large number of allelic variants including extended-spectrum β-lactamases (ESBL), non-ESBL and several not classified variants. SHV enzymes have evolved from a narrow- to an extended-spectrum of hydrolyzing activity, including monobactams and carbapenems, as a result of amino acid changes that altered the configuration around the active site of the β -lactamases. SHV-ESBLs are usually encoded by self-transmissible plasmids that frequently carry resistance genes to other drug classes and have become widespread throughout the world in several Enterobacteriaceae, emphasizing their clinical significance.

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Section: Expansion Toward New Ecological Nichessupporting

confidence: 74%

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

2016

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“…A further objective of the study was to screen E. coli and K. pneumoniae isolates for ESBL antibiotic resistance gene determinants. Despite the fact that ESBL resistance genes have most frequently been detected among E. coli and K. pneumoniae isolated from humans, clinical care and hospital facilities, a number of studies have also reported that ESBL resistance genes are harboured by E. coli and K. pneumoniae strains from food-producing animals, meat products as well as vegetables [9,11,14,38,[40][41][42][43][44]. In most studies, ESBL genes (blaCXT-M, blaTEM, blaSHV, blaOXA and blaCMY) were the most frequently detected resistant determinants in E. coli, K. pneumoniae and Salmonella species [6,14,37].…”

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnmentioning

confidence: 99%

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Montso

Dlamini

Kumar

et al. 2019

BioMed Research International

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Background. Extended spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae cause severe infections in humans which leads to complicated diseases. There is increasing evidence that cattle contribute to the development and spread of multidrug resistant pathogens and this raises public health concern. Despite this, data on the concurrence of ESBL producing pathogens in cattle, especially in the North-West province are rare. Therefore, the aim of the present study was to isolate, identify and characterise ESBL producing E. coli and K. pneumoniae species from cattle faeces and raw beef samples. Results. A total of 151 samples comprising 55 faeces samples and 96 raw beef samples were collected and 259 nonreplicative potential isolates of Enterobacteriaceae were obtained. One hundred and ninety-six isolates were confirmed as E. coli (114; 44%) and K. pneumoniae (82; 32%) species through amplification of uspA and uidA and ntrA gene fragments, respectively. Antimicrobial susceptibility test revealed that large proportions (66.7–100%) of the isolates were resistant to Amoxicillin, Aztreonam, Ceftazidime, Cefotaxime, and Piperacillin and were multidrug resistant isolates. Cluster analysis of antibiotic inhibition zone diameter data revealed close similarities between isolates from different sources or species thus suggested a link in antibiotic exposures. The isolates showing phenotypic resistance against ESBL antimicrobial susceptibility tests were screened for the presence of ESBL gene determinants. It was observed that 53.1% of the isolates harboured ESBL gene determinants. The blaTEM, blaSHV and blaCTX-M genes were detected in E. coli isolates (85.5%, 69.6%, and 58%, respectively) while blaCTX-M and blaOXA were detected in K. pneumoniae (40% and 42.9%, respectively). All the genetically confirmed ESBL producing E. coli and K. pneumoniae isolates were subjected to Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR analysis. Fingerprinting data revealed great similarities between isolates from different areas and sources which indicates cross-contamination between cattle and beef. Conclusion. This study revealed that cattle and its associated food products, beef in particular, harbour ESBL producing pathogens. And this warrants a need to enforce hygiene measures and to develop other mitigation strategies to minimise the spread of antibiotic resistant pathogens from animals to human.

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“…Microbial characterisation of a food reveals the level of hygiene and it is a key factor in assessing the quality and the safety of food. Even though, the isolation of Klebsiella strains from various foods have previously been reported (Park & Sanders, 1990;Kim et al,2015), to our knowledge, our study is the first report of the isolation K. oxytoca and K. pneumoniae which members of the Enterobacteriaceae, isolated from Hurma olive samples. When the results were obtained in this study, are taken into consideration, Hurma olives seem safe for human consumption as any other fruits and vegetables.…”

Section: Discussionmentioning

confidence: 71%

Microbial profile and bacterial characterisation of naturally debittered Hurma olives compared to non‐debittered Erkence variety during ripening period

Sözbilen

Baysal

2016

Int J of Food Sci Tech

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SummaryNaturally debittered Hurma olive is grown in a specific area in Karaburun peninsula in Turkey. It is characterised by its sweet taste and it differs from other varieties by losing its bitterness caused by phenolic compounds during its maturation period on the tree. Therefore, Hurma olive does not require any further debittering process to be served as table olive. This study was particularly interested in the comparison of the microbial profile of Hurma olive during its 8 weeks of maturation period in two subsequent harvest years and Erkence (not naturally debittered) olive. In addition, main bacterial profile of both Hurma and Erkence olives were isolated and identified. Aerobic mesophilic microorganism (AMM), lactic acid bacteria (LAB), Enterobacteriaceae, Pseudomonadaceae, Staphylococci, Micrococcaceae, yeasts and moulds (Y&Ms) were detected (counted and isolated) in the olive drupes during the maturation period. Isolated bacteria were identified as different spp. of Bacillaceae, Enterobacteriaceae, Micrococcoceae and Pseudomonadaceae.

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Prevalence and characterization of extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in ready-to-eat vegetables

Cited by 84 publications

References 35 publications

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Microbial profile and bacterial characterisation of naturally debittered Hurma olives compared to non‐debittered Erkence variety during ripening period

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