Prevalence and Characterization of Foodborne Pathogens in Dairy Cattle in the Eastern Part of Japan

Sasaki, Yohei; Murakami, Mariko; Haruna, Mika; Maruyama, Noriko; Mori, Tetsuya; Itô, Kazuo; Yamada, Yukiko

doi:10.1292/jvms.12-0327

Cited by 11 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most other comparable studies reported prevalences between 5% and 67.1% [10-14,18,22,23,28-32]. Since these studies vary in sampling design, culture methods and conditions, a direct comparison of the results is difficult.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence, quantitative load and genetic diversity of Campylobacter spp. in dairy cattle herds in Lithuania

et al. 2013

View full text Add to dashboard Cite

BackgroundCampylobacteriosis is a zoonotic disease, and animals such as poultry, pigs and cattle may act as reservoirs for Campylobacter spp. Cattle shed Campylobacter spp. into the environment and they can act as a reservoir for human infection directly via contact with cattle or their faeces or indirectly by consumption of contaminated food. The aim of this study was to determine the prevalence, the quantitative load and the genetic strain diversity of Campylobacter spp. in dairy cattle of different age groups.ResultsFaecal samples of 200 dairy cattle from three farms in the central part of Lithuania were collected and examined for Campylobacter. Cattle herds of all three farms were Campylobacter spp. positive, with a prevalence ranging from 75% (farm I), 77.5% (farm II) to 83.3% (farm III). Overall, the highest prevalence was detected in calves (86.5%) and heifers (86.2%). In contrast, the lowest Campylobacter prevalence was detectable in dairy cows (60.6%). C. jejuni, C. coli, C. lari and C. fetus subsp. fetus were identified in faecal samples of dairy cattle. C. upsaliensis was not detectable in any sample. The high counts of Campylobacter spp. were observed in faecal material of dairy cattle (average 4.5 log10 cfu/g). The highest numbers of Campylobacter spp. were found in faecal samples from calves (average 5.3 log10 cfu/g), whereas, faecal samples from cows harboured the lowest number of Campylobacter spp. (average 3.7 log10 cfu/g). Genotyping by flaA PCR-RFLP analysis of selected C. jejuni isolates showed that some genotypes were present in all farms and all age groups. However, farm or age specific genotypes were also identified.ConclusionsFuture studies are needed to investigate risk factors related to the degree of colonisation in cattle. Based on that, possible measures to reduce the colonisation and subsequent shedding of Campylobacter in cattle could be established. It is important to further investigate the epidemiology of Campylobacter in the cattle population in order to assess associated risks to public health.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In addition, indirect exposure to cattle faeces through environmental contamination is considered a high risk to humans [19-21]. Up to now, there is limited and controversial information on the influence of the age of cattle on the Campylobacter prevalence [6,12,14,22,23]. …”

Section: Introductionmentioning

confidence: 99%

Prevalence, quantitative load and genetic diversity of Campylobacter spp. in dairy cattle herds in Lithuania

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Other researchers reported prevalence between 5% and 78.5%. 25,26 Since sampling design, cultural methods and conditions were varied among these studies, a direct comparison of the results is troublesome. However, our data contribute to previous conversation that dairy cattle are significant assortment for Campylobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation, molecular identification and antimicrobial resistance patterns of Campylobacter species of dairy origin: first report from Bangladesh

et al. 2019

View full text Add to dashboard Cite

This study was aimed for isolation, identification and characterization of Campylobacter species from Bangladesh Agricultural University (BAU) dairy farm during the period of January to May, 2016. A total of 80 samples (faecal samples of calves, heifers and cows; milk samples of cows) were collected from Bangladesh Agricultural University dairy farm for isolation and identification of Campylobacter species by using cultural, biochemical and molecular methods. Moreover, the isolated Campylobacter species were subjected for antimicrobial susceptibility test. Campylobacter like organisms were presumptively identified in 20 samples. Isolates were biochemically positive to catalase and oxidase tests and in hippurate hydrolysis test some of the isolates (n=6) shown negative that indicated the isolates were C. coli and some of the test isolates (n=14) shown positive that indicated the isolates were C. jejuni. Campylobacter specific 16S rRNA genes were amplified from the isolates. Out of 20 isolated Campylobacter 14 (17.5%) were detected as C. jejuni and the rest 6 (7.5%) were detected as C. coli by cdtC gene based multiplex PCR assay. C. jejuni were resistant to amoxicillin, erythromycin, azithromycin and susceptible to gentamicin, ciprofloxacin, norfloxacin and streptomycin. Furthermore, C. coli were resistant to amoxicillin and erythromycin and susceptible to gentamycin, ciproflaxacin. Out of 20 Campylobacter isolates, 57.14% C. jejuni and 33.33% C. coli were identified as multidrug resistant. To the best of our knowledge, this study has brought the first report on the occurrence of Campylobacter species with their antibiogram profiles in any dairy farm of Bangladesh.

show abstract

“…For example, studies have revealed a range of Campylobacter prevalence (up to 70%) in cattle. 31,[74][75][76] Furthermore, although the prevalence of Campylobacter in cattle carcasses and milk samples can be relatively low in comparison to other sources, studies have shown that approximately 15% of beef carcasses/meat can be contaminated with these pathogens. 38,92 In addition, a Campylobacter prevalence of 41.7%, 10.2%, and 4.6% had been previously reported in raw bulk tank milk in Northern Italy, Pakistan, and Poland, respectively, while 6.25% of raw cow milk samples from retail stores were contaminated with Campylobacter in Iran.…”

mentioning

confidence: 99%

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Kashoma

Kassem

John

et al. 2016

Microbial Drug Resistance

View full text Add to dashboard Cite

Campylobacter species are commonly transmitted to humans through consumption of contaminated foods such as milk and meat. The aim of this study was to investigate the prevalence, antimicrobial resistance, and genetic determinants of resistance of Campylobacter isolated from raw milk and beef carcasses in Tanzania. The antimicrobial resistance genes tested included bla OXA-61 (ampicillin), aph-3-1 (aminoglycoside), tet(O) (tetracycline), and cmeB (multi-drug efflux pump). The prevalence of Campylobacter was 9.5% in beef carcasses and 13.4% in raw milk, respectively. Using multiplex-polymerase chain reaction (PCR), we identified 58.1% of the isolates as Campylobacter jejuni, 30.7% as Campylobacter coli, and 9.7% as other Campylobacter spp. One isolate (1.6%) was positive for both C. jejuni and C. coli specific PCR. Antimicrobial susceptibility testing using the disk diffusion assay and the broth microdilution method showed resistance to: ampicillin (63% and 94.1%), ciprofloxacin (9.3% and 11.8%), erythromycin (53.7% and 70.6%), gentamicin (0% and 15.7%), streptomycin (35.2% and 84.3%), and tetracycline (18.5% and 17.7%), respectively. Resistance to azithromycin (42.6%), nalidixic acid (64.8%), and chloramphenicol (13%) was determined using the disk diffusion assay only, while resistance to tylosin (90.2%) was quantified using the broth microdilution method. The bla OXA-61 (52.6% and 28.1%), cmeB (26.3% and 31.3%), tet(O) (26.3% and 31.3%), and aph-3-1 (5.3% and 3.0%) were detected in C. coli and C. jejuni. These findings highlight the extent of antimicrobial resistance in Campylobacter occurring in important foods in Tanzania. The potential risks to consumers emphasize the need for adequate control approaches, including the prudent use of antimicrobials to minimize the spread of antimicrobial-resistant Campylobacter.

show abstract

Prevalence and Characterization of Foodborne Pathogens in Dairy Cattle in the Eastern Part of Japan

Cited by 11 publications

References 21 publications

Prevalence, quantitative load and genetic diversity of Campylobacter spp. in dairy cattle herds in Lithuania

Prevalence, quantitative load and genetic diversity of Campylobacter spp. in dairy cattle herds in Lithuania

Isolation, molecular identification and antimicrobial resistance patterns of Campylobacter species of dairy origin: first report from Bangladesh

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Contact Info

Product

Resources

About