Prevalence and Characterization of Gentamicin Resistance Genes in Escherichia coli Isolates from Beef Cattle Feces in Japan

Yamamoto, Shiori; Kitagawa, Wataru; Nakano, Motoki; Asakura, Hiroshi; Nakayama, Tadasi; Iwabuchi, Eriko; Sone, Toshio; Asano, Kozo

doi:10.1007/s00284-022-02913-6

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…While there has been no report of the risk to human health, bacitracin might contribute to the resistance of colistin-resistant E. coli [18,24]. On the other hand, gentamicin is an aminoglycoside-type antibiotic that is commonly used in the veterinary practices, which exhibits efficacy against both gram-positive and gram-negative bacteria [25]. Studies Liu et al (2022) [26] and Sabir et al (2014) [27] seasons.…”

Section: Discussionmentioning

confidence: 99%

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Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

Paramitadevi,

Priadi,

Rahmatika

et al. 2024

E3S Web of Conf.

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Antibiotic-resistant E. coli is a growing concern in various settings, but environmental studies are rare compared to clinical research on human and animal health. This study aimed to identify the prevalence of E. coli bacteria resistant to different antibiotics in the environment by examining E. coli resistant to cefotaxime isolated from ruminant stool, feed, and compost. The phenotyping test was conducted through antibiotic susceptibility test using Kirby-Bauer disk-diffusion method, followed by the One-Way variance (ANOVA) analysis of the antibiotic susceptibility test results. Of the 12 isolates exposed to cefotaxime, six showed resistance to this antibiotic, and all isolates, including those resistant to cefotaxime, were resistant to eight out of ten types of antibiotics. All isolates had resistance to at least two to five types of antibiotics. The phenotypic pattern between fecal isolates and non-fecal isolates did not differ significantly, except for the antibiotics amoxicillin (p≤0.05) and ampicillin (p≤0.05). The overlapping resistance patterns observed in animal feed, animal stool, and compost suggest a potential link between their microbiological compositions.

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Section: Discussionmentioning

confidence: 99%

“…Studies Liu et al (2022) [26] and Sabir et al (2014) [27] seasons. Bacteria resistant to gentamicin from livestock environments raises concerns, as it can potentially enter the meat supply chain for human consumption [25].…”

Section: Discussionmentioning

confidence: 99%

Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

Paramitadevi,

Priadi,

Rahmatika

et al. 2024

E3S Web of Conf.

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show abstract

“…Especially, gentamicin is recommended as the empirical parenteral treatment for children with community-acquired urinary tract infections and as a crucial antibiotic for preventing orthopedic infections (Mosselhy et al, 2018;Roldan-Masedo et al, 2019). However, due to the widespread use of antibiotics, gentamicin-resistant E. coli is an ever-increasing problem that causes infection in both human health and animal feeding (Salas-Mera et al, 2017;Yamamoto et al, 2022). Therefore, restoration of gentamicin-mediated killing is highly demanded.…”

Section: Introductionmentioning

confidence: 99%

Glutamine potentiates gentamicin to kill lab-evolved gentamicin-resistant and clinically isolated multidrug-resistant Escherichia coli

Chen

Peng

et al. 2022

Front. Microbiol.

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IntroductionGentamicin is a conventional antibiotic in clinic. However, with the wide use of antibiotics, gentamicin-resistant Escherichia coli (E. coli) is an ever-increasing problem that causes infection in both humans and animals. Thus, it is especially important to restore gentamicin-mediated killing efficacy.MethodE. coli K12 BW25113 cells were passaged in medium with and without gentamicin and obtain gentamicin-resistant (K12-RGEN) and control (K12-S) strains, respectively. Then, the metabonomics of the two strains were analyzed by GC-MS approach.ResultsK12-RGEN metabolome was characterized as more decreased metabolites than increased metabolites. Meantime, in the most enriched metabolic pathways, almost all of the metabolites were depressed. Alanine, aspartate and glutamate metabolism and glutamine within the metabolic pathway were identified as the most key metabolic pathways and the most crucial biomarkers, respectively. Exogenous glutamine potentiated gentamicin-mediated killing efficacy in glutamine and gentamicin dose-and time-dependent manners in K12-RGEN. Further experiments showed that glutamine-enabled killing by gentamicin was effective to clinically isolated multidrug-resistant E. coli.DiscussionThese results suggest that glutamine provides an ideal metabolic environment to restore gentamicin-mediated killing, which not only indicates that glutamine is a broad-spectrum antibiotic synergist, but also expands the range of metabolites that contribute to the bactericidal efficiency of aminoglycosides.

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Gentamicin resistance to Escherichia coli related to fatty acid metabolism based on transcriptome analysis

et al. 2023

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Antibiotic overuse and misuse have promoted the emergence and spread of antibiotic-resistant bacteria. Increasing bacterial resistance to antibiotics is a major healthcare problem, necessitating elucidation of antibiotic resistance mechanisms. In this study, we explored the mechanism of gentamicin resistance by comparing the transcriptomes of antibiotic-sensitive and -resistant Escherichia coli. A total of 410 differentially expressed genes were identified, of which 233 (56.83%) were up-regulated and 177 (43.17%) were down-regulated in the resistant strain compared with the sensitive strain. Gene ontology (GO) analysis classifies differential gene expression into three main categories: biological processes, cellular components and molecular functions. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that the up-regulated genes were enriched in eight metabolic pathways, including fatty acid metabolism, which suggests that fatty acid metabolism may be involved in the development of gentamicin resistance in E. coli. This was demonstrated by measuring the acetyl-CoA carboxylase activity, plays a fundamental role in fatty acid metabolism, was increased in gentamicin-resistant E. coli. Treatment of fatty acid synthesis inhibitor, triclosan, promoted gentamicin-mediated killing effificacy to antibiotic-resistant bacteria. We also found that exogenous addition of oleic acid, which involved in fatty acid metabolism, reduced E. coli sensitivity to gentamicin. Overall, our results provide insight into the molecular mechanism of gentamicin resistance development in E. coli.

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Prevalence and Characterization of Gentamicin Resistance Genes in Escherichia coli Isolates from Beef Cattle Feces in Japan

Cited by 3 publications

References 20 publications

Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

Glutamine potentiates gentamicin to kill lab-evolved gentamicin-resistant and clinically isolated multidrug-resistant Escherichia coli

Gentamicin resistance to Escherichia coli related to fatty acid metabolism based on transcriptome analysis

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