Prevalence and Characterization of Shiga-Toxin O157:H7 and Non-O157:H7 Enterohemorrhagic<i>Escherichia Coli</i>Isolated from Different Sources

Selim, Samy; Ahmed, Salwa F.; Aziz, Abdel; Zakaria, Ahmad; Klena, John D.; Pangallo, Domenico

doi:10.5504/bbeq.2013.0031

Cited by 30 publications

(20 citation statements)

References 41 publications

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“…Staphylococcal enterotoxins (SEs) are encoded by several genes; however, sea, seb, sec, sed and see were the most common genes implicated in cases of food poisoning worldwide [5]. The seriousness of E. coli and S. aureus milk-borne food poisoning was exaggerated with the emergence of Multi drug resistant (MDR) isolates worldwide, which may pose an additional threat to human health [11,12].…”

Section: Introductionmentioning

confidence: 99%

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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Introduction: Milk is an important food in Egypt and most of it is sold as raw milk in informal markets. Methodology: This study was conducted to investigate the public health risks of Escherichia coli and Staphylococcus aureus in milk sold in informal markets in Egypt. A total of 121 milk samples were analyzed for occurrence, virulence genes and antibiotic resistance of E. coli and S. aureus. Results: A total of 35/121 (28.9%) of milk samples were contaminated with 16/121 (13.2%) E. coli, 22/121 (18.2%) S. aureus, and 3/121 (2.5%) both isolates. Shiga-toxin producing E. coli (STEC), Enterotoxigenic E. coli (ETEC) and Enterotoxigenic S. aureus were detected in 5/121 (4.1%), 2/121 (1.7%) and 8/121 (6.6%) of the examined milk samples, respectively. Multiple drug resistances (MDRs) were showed by 14/16 (87.5%) and 21/22 (95.5%) of E. coli and S. aureus isolates, respectively. E. coli isolates showed high resistance for cephalothin (87.5%), ampicillin (68.8%) and tetracycline (68.8%), but were sensitive for gentamicin and chloramphenicol. Resistance phenotypes of E. coli were diverse; however, STEC isolates were significantly associated with co-resistance to cephalothin, ampicillin and tetracycline (P< 0.05). Two (9.1%) of S. aureus isolates were methicillin-resistant (MRSA) but sensitive to gentamicin (GS-MRSA). Five (22.7%) of S. aureus isolates were gentamicin-resistant methicillin-sensitive S. aureus (GR-MSSA). S. aureus isolates also showed high resistance for ampicillin (100%), tetracycline (90.1%) and sulfamethoxazole-trimethoprim (90.1%). Conclusion: These findings highlighted the potential public health hazards of E. coli and S. aureus pathogens in raw milk sold in informal markets in Egypt.

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Section: Introductionmentioning

confidence: 99%

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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“…In our study, more than half of urine samples were positive to E. coli and 23 E. coli O157 isolates were obtained. Previous studies conducted in various parts of the world showed that the prevalence rate of E. coli O157 was about 10% [21,22]. In this study, the contamination rates were only approximate values.…”

Section: Discussionmentioning

confidence: 70%

Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections

Xie

Dong²,

Zhu³

et al. 2017

Urol Int

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Background:Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. Objectives: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E. coli O157 based on the prevalence of urinary tract infection (UTI) in Hubei, China. Results: We obtained 23 (8.07%) E. coli O157 isolates from 285 UTI patients in Hubei, China. All isolates were subjected to antibiotic susceptibility analysis, and molecular typing was performed using ERIC-PCR and MLST. Antimicrobial susceptibility results indicated that most strains were resistant to penicillin (95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All isolates were discovered to be multiresistant (resistance to more than 3 antibiotics). Genetic variability analysis showed that all of the isolates were grouped into 4 clusters both by ERIC-PCR and MLST. Conclusion: Our findings demonstrated the presence of E. coli O157 in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of E. coli O157 risk assessment in UTIs.

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“…EHEC E. coli was also identified from different sources in Isamailia, Egypt (Selim et al, 2013 (Sha et al, 2013). Caenorhabditis elegans was fed with Salmonella and a control (E. coli).…”

Section: Nucleic Acids-based Detection Methodsmentioning

confidence: 99%

Detection and Occurrence of Indicator Organisms and Pathogens

Kronlein¹,

Stedtfeld²,

Samhan³

et al. 2014

Water Environment Research

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This review summarizes some key literature published in 2013 that pertains to the detection and occurrence of pathogens and indicators of waterborne pathogens. Particular emphasis was placed on emerging detection methods that when fully developed may have an impact on the application of molecular tools to characterize the microbial quality of water. As such, this review is organized into the following topics: i) nucleic acids‐based detection methods, ii) sequencing‐based detection methods, iii) culturing combined to molecular methods, and iv) sample processing.

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Prevalence and Characterization of Shiga-Toxin O157:H7 and Non-O157:H7 EnterohemorrhagicEscherichia ColiIsolated from Different Sources

Cited by 30 publications

References 41 publications

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections

Detection and Occurrence of Indicator Organisms and Pathogens

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