Prevalence and Characterization of Shigatoxigenic Escherichia Coli in Broiler Birds in Mymensingh

Abstract

Shigatoxigenic Escherichia coli (STEC) are major food-borne pathogens. They transmit to human through contaminated meat and meat products of animals and poultry, and frequently associated with various types of human illness including haemolytic uremic syndrome. This preliminary study showed the prevalence of STEC in 60 cloacal swab samples of live healthy broiler chickens collected randomly when sold at a wholesale market in Mymensingh district of Bangladesh. Isolation and identification of E. coli was carried out using Eosin Methylene Blue (EMB) agar media and 16S rRNA gene specific polymerase chain reaction (PCR). Among the 60 samples, 49 (81.67%) were found positive to E. coli. These E. coli isolates were screened for the detection of STEC by PCR using stx1 and stx2 gene specific primers. Among the 49 positive samples, 5 (10.20%) were found positive for stx1 gene, and 26 (53.06%) were positive for stx2 gene. In addition, 6 (12.24%) isolates were found positive to both stx1 and stx2 genes, and the remaining 12 (22.46%) were negative. The high prevalence ofSTEC in the broiler chicken alarms the public health impact as the people are always in close contact with these live broiler chickens in the open market as well as processing of meat at home before cooking. However, further studies are required to uncover the major source(s) for the transmission of STEC to human in ruralBangladesh.

Molecular Detection and Antibiogram of Shiga Toxin Producing Escherichia Coli (Stec) Isolated From Diarrheic Children

“…In this study, characteristics colonies of E. coli were observed on EMB agar, MC agar, which was similar to the findings of several previous reports (Nazir et al, 2005;Nazir, 2007;Hassan et al, 2014;Mamun et al, 2016;Tanzin et al, 2016). In Gram's staining method, the isolated bacteria exhibited pink, small rod shaped Gramnegative bacilli.…”

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

“…Colony growth character on different media exhibited characteristics reaction. In this study, colony characteristics of E. coli observed on EMB and MC agars were similar to the findings of other authors (Rehman et al, 2014;Hassan et al, 2014;Mamun et al, 2016;Tanzin et al, 2016;Elafify et al, 2016;Islam et al, 2016).…”

“…This indicated the isolates were E. coli. The isolates also revealed positive reaction in MR test and Indole test but negative reaction in VP test, which was supported by several authors (Hassan et al, 2014;Mamun et al, 2016;Tanzin et al, 2016;Elafify et al, 2016;Islam et al, 2016).…”

Detection of Multidrug Resistant and Shiga Toxin Producing <i>Escherichia coli</i> (STEC) From Apparently Healthy Broilers in Jessore, Bangladesh

“…The EHEC strains are one of subset of Shiga toxin (Stx) producing E. coli (STEC) strains which is isolated from patients [7]. STEC has recently been found from broiler in Bangladesh [8]. Food contaminated with STEC strains is the main cause of human infections [9].…”