Prevalence and contamination levels of &lt;i&gt;listeria monocytogenes&lt;/i&gt; in ready-to-eat foods in Tokyo, Japan

Shimojima, Yukako; Ida, Miki; Nakama, Akiko; Nishino, Yukari; Fukui, Rui; Kuroda, Sumiyo; Hirai, Akihiko; Kai, Akemi; Sadamasu, Kenji

doi:10.1292/jvms.15-0708

Cited by 26 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the most common serotypes isolated from RTE food (including natural cheese, meat products, seafood and pickled vegetables) in Japan were 1/2a (47.6%), 1/2b (20.6%) and 4b (14.3%). 34 Similar findings were reported in other countries of the northern hemisphere. In Poland, Korsak et al, 35 analyzed 471 L. monocytogenes strains isolated from different foods and found the following serotype distribution: 1/2a, 54.4%; 1/2c, 25.5%; 1/2b, 12.5% and 4b 7.6%.…”

Section: Discussionsupporting

confidence: 85%

Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay

Braga¹,

Vázquez²,

Vico³

et al. 2017

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment. L. monocytogenes was isolated from 11.2% of samples. The highest prevalence was observed in frozen foods (38%), followed by cheese (10%). 1/2b and 4b were the most frequently identified serotypes. In six of 236 analyzed establishments we successively recovered L. monocytogenes isolates belonging to the same serotype. Most of them corresponded to serotype 1/2b. Pulsed-field gel electrophoresis profiles suggest that at least 33% of L. monocytogenes 1/2b isolates are genetically related and that may remain viable for prolonged periods. The observed prevalence of L. monocytogenes was lower than reported in neighboring countries. Our findings highlight the role that frozen foods may play in the spread of this pathogen, and the relevance of serotypes 1/2b and 4b.

show abstract

Section: Discussionsupporting

confidence: 85%

Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay

Braga¹,

Vázquez²,

Vico³

et al. 2017

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…In this study, we assessed 570 food samples in China from 2014 to 2016, 39 RTE foods and 5 pasteurized milk samples (7.7%) tested positive for L. monocytogenes, concurrent with contamination of L. monocytogenes in RTE foods reported in Beijing (Wang W. et al, 2015) but significantly greater than that of RTE foods in Estonia (Koskar et al, 2019) and Tokyo, Japan (Shimojima et al, 2016). However, Wang et al (2018) reported that 16.2% of samples were contaminated with L. monocytogenes in retail outlets selling RTE foods and restaurants serving mutton in Zigong, Sichuan Province.…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity, Characteristics, and Public Health Implications of Listeria monocytogenes in Ready-to-Eat Foods and Pasteurized Milk in China

Chen

Wang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Listeria monocytogenes is a foodborne pathogen with a high mortality rate in humans. This study aimed to identify the pathogenic potential of L. monocytogenes isolated from ready-to-eat (RTE) foods and pasteurized milk in China on the basis of its phenotypic and genotypic characteristics. Approximately 7.7% (44/570) samples tested positive for L. monocytogenes among 10.8% (39/360) RTE and 2.4% (5/210) pasteurized milk samples, of which 77.3% (34/44) had < 10 MPN/g, 18.2% (8/44) had 10-110 MPN/g, and 4.5% (2/44) had > 110 MPN/g. A total of 48 strains (43 from RTE foods and five from milk samples) of L. monocytogenes were isolated from 44 positive samples. PCRserogroup analysis revealed that the most prevalent serogroup was II.2 (1/2b-3b-7), accounting for 52.1% (25/48) of the total, followed by serogroup I.1 (1/2a-3a) accounting for 33.3% (16/48), serogroup I.2 (1/2c-3c) accounting for 12.5% (6/48), and serogroup II.1 (4b-4d-4e) accounting for 2.1%. All isolates were grouped into 11 sequence types (STs) belonging to 10 clonal complexes (CCs) and one singleton (ST619) via multi-locus sequence typing. The most prevalent ST was ST87 (29.2%), followed by ST8 (22.9%), and ST9 (12.5%). Virulence genes determination showed that all isolates harbored eight virulence genes belonging to Listeria pathogenicity islands 1 (LIPI-1) (prfA, actA, hly, mpl, plcA, plcB, and iap) and inlB. Approximately 85.4% isolates carried full-length inlA, whereas seven isolates had premature stop codons in inlA, six of which belonged to ST9 and one to ST5. Furthermore, LLS (encoded by llsX gene, representing LIPI-3) displays bactericidal activity and modifies the host microbiota during infection. LIPI-4 enhances neural and placental tropisms of L. monocytogenes. Results showed that six (12.5%) isolates harbored the llsX gene, and they belonged to ST1/CC1, ST3/CC3, and ST619. Approximately 31.3% (15/48) isolates (belonging to ST87/CC87 and ST619) harbored ptsA (representing LIPI-4), indicating the potential risk of this pathogen.

show abstract

“…ice-cream contaminated with L. monocytogenes was recalled in the US and in other countries, including Qatar or collagen protein bar and Bite products recalled from 42 countries. The concerns regarding listeriosis are not only related to the high frequency of detection of L. monocytogenes due to the bacterial ecology, but also to the category of contaminated products (usually 'ready to eat products'), which are consumed without other previous treatment (Ajayeoba et al 2016;Shimojima et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Inhibition ofListeria monocytogenesby a formulation of selected dairy starter cultures and probiotics in anin vitromodel

Iulietto

Sechi

Cella

et al. 2018

Italian Journal of Animal Science

View full text Add to dashboard Cite

Three strains of lactic acid bacteria (LAB) and a commercial probiotic were selected to evaluate their in vitro activity towards Listeria monocytogenes. The strains Lactococcus lactis ssp. lactis, strain 340, L. lactis ssp. lactis, strain 16; Lactobacillus casei ssp. casei, strain 208 and Enterococcus faecium UBEF-41 were inoculated into skim milk and brain heart infusion broth (BHI) to get an initial Lactococcus: Lactobacillus: E. faecium UBEF-41 ratio of 2:1:1 and a concentration of approximately 7 log cfu mL À1 until challenge vs. pathogen. L. monocytogenes ATCC 7644 was also inoculated in same media to get approximately 4 log cfu mL À1 . Growth curves in skim milk and BHI at 4, 10 and 30 C, respectively were studied for: (i) LAB formulation; (ii) L. monocytogenes and (iii) LAB vs. L. monocytogenes. When challenged with LAB, at 30 C in milk, L. monocytogenes was not detectable after day-3 and in BHI it decreased below log cfu mL À1 after day-5. At 10 and 4 C, in both media, L. monocytogenes counts were always significantly lower (p < .001) than the counts of L. monocytogenes alone from day-2 for milk at 4 C and BHI at 10 C and from day-7 for BHI at 4C and milk at 10 C. In conclusion, the proposed formulation was able to limit L. monocytogenes in vitro growth, even at refrigeration temperature. ARTICLE HISTORY

show abstract

Prevalence and contamination levels of <i>listeria monocytogenes</i> in ready-to-eat foods in Tokyo, Japan

Cited by 26 publications

References 8 publications

Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay

Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay

Heterogeneity, Characteristics, and Public Health Implications of Listeria monocytogenes in Ready-to-Eat Foods and Pasteurized Milk in China

Inhibition ofListeria monocytogenesby a formulation of selected dairy starter cultures and probiotics in anin vitromodel

Contact Info

Product

Resources

About