Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016

Durmaz, Rıza; Bakkaloğlu, Zekiye; Ünaldı, Özlem; Karagöz, Alper; Korukluoğlu, Gülay; Kalaycioglu, Atila T.; Acar, Sumeyra; Kılıç, Selçuk

doi:10.1002/jmv.24945

Cited by 11 publications

(11 citation statements)

References 44 publications

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“…Rotavirus was detected among children in all age groups. According to studies from different localities, it was found that rotavirus infection usually occurred in children younger than 3 years of age . In agreement with this, the finding revealed children less than 3 years of age (85.7%) had a higher rate of rotavirus infection than children over 3 years of age (13.3%), which children of the 12‐to‐23 month group (45.1%) being the most frequently affected.…”

Section: Discussionsupporting

confidence: 57%

Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

Chan-it

Chanta

2017

Journal of Medical Virology

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Human group A rotavirus is a major contagious virus causing gastroenteritis in children. Molecular epidemiological study of group A rotavirus infections in hospitalized children was performed by multiplex RT-PCR during 2015-2016 in Chiang Rai, Thailand. G- and P-genotypes of positive rotavirus samples were further analyzed by one-step and two-step multiplex RT-PCR methods. Among 270 fecal specimens tested, rotavirus was the most prevalent (33.7%), followed by norovirus GII (4.1%), adenovirus (3%), and astrovirus (1.5%). Infection was common in patients aged 12-23 months (45.1%) and occurred mostly in children under 3 years of age (85.7%). The highest peak was in a hot month, March (64.8%). G9P[8] emerged as the most predominant (79.1%), followed by G3P[8] (13.2%), G1P[8] (3.3%), and mixed G-types (4.4%). Interestingly, Chiang Rai G9 strains were clustered within a distinct lineage VII including G9 recently reported since 2010-2015. G9-VII also contained four to five unique amino acid substitutions in the VP7 proteins compared with those of the G9 candidate vaccine strain RVA/Human-tc/IND/116E/1985/G9P[11] and the prototype RVA/Human-wt/USA/WI61/1983/G9P[8], defining the G9-VII as a novel variant. G3 strains were closely related to the "new G3P[8] reassortant variant" with an equine-like VP7 gene that emerged in several countries. This study contributes to the understanding of the genetic diversity, providing scientific support for future vaccine strategies to reduce the morbidity and mortality.

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Section: Discussionsupporting

confidence: 57%

Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

Chan-it

Chanta

2017

Journal of Medical Virology

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“…Although these results do not reflect the society incidence, a decrease can be seen in G9 frequency (33). Also a rotavirus surveillance study from Turkey in 2014-2016 reported that the most prevalent genotype was G1P[8] (24.6%) followed by G3P [8] (19.6%) and G9P [8] (12.2%) (34). The samples of the present study belong to year 2012, which is one of the limitations of our study, however it can be seen from the literature that genotype G9 was the predominantly circulating genotype around 2012.…”

Section: Discussionmentioning

confidence: 99%

Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara

Koçak

Aydın

Matsumoto

et al. 2019

Revista Romana De Medicina De Laborator

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Introduction: Rotavirus infection is a major cause of morbidity and mortality in infants and young children with diarrhea throughout the world. Material and Methods: In this study, we aimed to determine the detection rate of rotavirus infection in 181 children less than 5 years of age presenting with acute gastroenteritis and admitted to a tertiary care hospital in Ankara, Turkey, from April to November 2012. We documented the epidemiological data by elucidating the prevalent genotypes. Stool specimens were collected, and rotavirus antigen in the samples was detected using ELISA. G and P genotypes were determined by RT-PCR via type specific primers. The nucleotide sequence of the concerned genes was determined by Sanger sequencing and phylogenetic analysis was performed by neighbor-joining method. Results: Of the 181 samples, 28 (15.5%) were positive for the rotavirus antigen. Twenty-seven samples were positive for G genotypes and 21 were positive for P genotypes. Genotypes G1 (7.1%), G2 (7.1%), G3 (7.1%), G4 (3.6%), G9 (71.5%) and P4 (3.6%), P8 (71.4%) were identified. Genotype G9P[8] (50%) was predominant in the combination of G and P genotypes. Most of the G9 strains of this study formed an independent cluster in Lineage III, except two strains which clustered with an Ethiopian G9 strain of 2012. Conclusions: It seems that during 2012 season, genotype G9P[8] increased significantly in Ankara due to a new circulating strain of G9.

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“…Çalışma süresinde ilk iki yılda görülmeyen G3P8 izolatının 2015 ve 2016 yıllarında sırasıyla %18.7, %13.5 oranlarında görüldüğü saptanmıştır. Durmaz ve arkadaşlarının yaptıkları sürveyans çalışmasının devamı niteliğindeki çalışmada 27 , bizim çalışmamızla uyumlu olarak 2014-2016 yıllarında G1P8 ve G3P8 izolatlarında artış ve G9P8, G2P4 izolatlarında azalma olduğu bildirilmiştir. Rotavirüs izolatlarının genotip dağılımındaki bu değişim, oluşan nokta mutasyonları ve hayvan izolatlarının insan izolatlarına olan etkisi sonucu yeniden ortaya çıkan genetik yapılanma nedeniyle gelişmektedir 28 .…”

Section: Discussionunclassified

Rotavirus Prevalence and Genotype Distribution in Children with Acute Gastroenteritis in Adana Province

Gündeşli̇oğlu¹,

Kocabaş²,

Haytoğlu³

et al. 2018

Mikrobiyol Bul

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Rotavirus is the most common agent of acute gastroenteritis in children under five years of age and still a major cause of mortality and morbidity mainly in devoloping countries. The aim of this study was to determine the circulating rotavirus genotypes in children less than five years of age with acute gastroenteritis in Adana province, before the introduction of the rotavirus vaccine into the national immunization programme. Fecal specimens were collected from children enrolled between December 2012 and November 2016 and rotavirus antigen was searched by a rapid immunochromatographic test method. The antigen positive samples were G and Pgenotyped by hemi-nested type-specific multiplex reverse-transcriptase polymerase chain reaction (RT-PCR). Rotavirus antigen positive fecal specimens from 201 patients of 107 (53.3%) boys and 94 (46.8%) girls were included in this study. Rotavirus infection was most frequently detected in patients with gastroenteritis between the ages of 12 and 23 months. It has been determined that 45% of rotavirus gastroenteritis was determined in children under two years of age. Rotavirus gastroenteritis was more frequent between September and April, but the highest incidence was detected in January. Of 201 rotavirus antigen-positive stool specimens, 167 were PCR positive. Five common G (G1, G2, G3, G4 and G9) and two common P genotypes (P[8] and P[4]) were determined as 91.6% and 96.4% of the isolates, respectively. The most prevalent G and P genotype combination was G1P8 with a frequency of 29.9%, followed by 21% G9P8 About 78% of rotavirus isolates were classified into five common G-P combination genotypes, including G1P8, G9P8 G3P8, G2P4, and G2P8. During the study period, there were significant changes in the distribution of rotavirus genotype. The prevalence of G9P8 genotype sharply decreased from 40% to 8.1% and the prevalence of G1P8 genotype increased from 21.3% to 48.6%. Although no G3P8 isolates were detected in the first two years of the study, this genotype was detected in 18.5% and 13.5% of the samples in 2015 and 2016, respectively. There were no relationship between the age onset of the disease, sex, dehydration development risk and rotavirus genotype. The results from this study indicated that the predominant circulating strains of rotavirus determined in clinical specimens were G1P8 and G9P8. Significant changes were observed in the prevalence of the most common rotavirus isolates over the years. Rotavirus surveillance is important to determine the circulating strains of rotavirus to assess vaccine effectiveness, to monitor vaccine failures, and to detect potential emerging strains.

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Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016

Cited by 11 publications

References 44 publications

Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara

Rotavirus Prevalence and Genotype Distribution in Children with Acute Gastroenteritis in Adana Province

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