Prevalence and genetic characteristics of Blastocystis hominis and Cystoisospora belli in HIV/AIDS patients in Guangxi Zhuang Autonomous Region, China

Xu, Ning; Jiang, Zhihua; Liu, Hua; Jiang, Yanyan; Wang, Zunfu; Zhou, Dongsheng; Shen, Yujuan; Cao, Jianping

doi:10.1038/s41598-021-94962-3

Cited by 5 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In neighboring countries, optical microscopy revealed Blastocystis rates of 32.1% in Algeria, 22.1% in Libya, and 13% in Tunisia [ 1 , 2 , 44 ]. The primary mode of Blastocystis transmission is the fecal-oral route, often occurring through contact with contaminated objects, foods, and drinking water, and even with contact with infected animals [ 12 , 31 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several risk factors, including age, gender, personal hygiene, education level, socio-economic status, parasitosis history, and health infrastructure access, have been associated with Blastocystis infection [ 11 , 66 ]. However, our study showed no significant association between Blastocystis sp.…”

Section: Discussionmentioning

confidence: 99%

“…shows a global distribution, but the prevalence varies widely between countries, ranging from 7% to 50% in developed nations to 55% to 100% in developing countries [ 8 , 26 , 65 ]. Several studies suggest associations with risk factors like age, gender, personal hygiene, education, socioeconomic status, parasitic history, sanitation, and proximity to animals [ 12 , 66 ]. The protozoan’s pathogenicity remains under debate, partly due to its genetic diversity, hinting at the existence of pathogenic and non-pathogenic subtypes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones’ culture medium

Boutahar,

Belaouni,

Ibrahimi

et al. 2023

Parasite

View full text Add to dashboard Cite

Blastocystosis is an infection caused by Blastocystis sp., which colonizes the digestive tract of various hosts, including humans, although its pathogenicity is debated. It is crucial to detect and distinguish the different forms of Blastocystis to understand better its impact on human health and its epidemiological evolution. This study evaluated three diagnostic methods on 105 stool samples: direct examination, culture in Jones’ medium, and conventional PCR. PCR is considered the gold standard and revealed a high prevalence of Blastocystis (67.62%) compared to direct examination (20.95%) and culture in Jones’ medium (51.43%). Although the sensitivity of direct examination and culture was 31% and 76.1%, respectively, their specificity was 100%. No significant risk factors were identified. A statistically significant association was observed between Blastocystis infection and abdominal pain. Microscopic analysis revealed various morphological forms. Molecular diagnosis is an essential tool to determine the true prevalence of Blastocystis, and studying the different forms of this microorganism will contribute to a better understanding of its biological cycle and, therefore, the impact of this emerging infection on human health.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones’ culture medium

Boutahar,

Belaouni,

Ibrahimi

et al. 2023

Parasite

View full text Add to dashboard Cite

show abstract

“…The prevalence of HIV/AIDS varies widely across China 1 2. Guangxi Zhuang Autonomous Region, the only minority region in southern China, is a serious HIV/AIDS hotspot; in the past decade, this region had a much higher HIV/AIDS prevalence than any other Chinese coastal or inland province 3 4. Therefore, the public health administration in Guangxi is attempting to expand the scale of HIV screening to diagnose HIV-infected patients at an early stage and provide highly active antiretroviral therapy (HAART) promptly to reduce HIV/AIDS mortality and transmission,5 6 especially in high-risk populations 7…”

Section: Introductionmentioning

confidence: 99%

Diagnostic performance evaluation of urine HIV-1 antibody rapid test kits in a real-life routine care setting in China

Lu,

Chen,

Liang

et al. 2024

BMJ Open

View full text Add to dashboard Cite

ObjectivesTo evaluate the diagnostic performance of urine HIV antibody rapid test kits in screening diverse populations and to analyse subjects’ willingness regarding reagent types, purchase channels, acceptable prices, and self-testing.DesignsDiagnostic accuracy studiesParticipantsA total of 2606 valid and eligible samples were collected in the study, including 202 samples from female sex workers (FSWs), 304 persons with injection drug use (IDU), 1000 pregnant women (PW), 100 subjects undergoing voluntary HIV counselling and testing (VCT) and 1000 students in higher education schools or colleges (STUs). Subjects should simultaneously meet the following inclusion criteria: (1) being at least 18 years old and in full civil capacity, (2) signing an informed consent form and (3) providing truthful identifying information to ensure that the subjects and their samples are unique.ResultsThe sensitivity, specificity and area under the curve (AUC) of the urine HIV-1 antibody rapid test kits were 92.16%, 99.92% and 0.960 (95% CI: 0.952 to 0.968, p<0.001), respectively, among 2606 samples collected during on-site screenings. The kits showed good diagnostic performance in persons with IDU (AUC, 1.000; 95% CI, 1.000 to 1.000, p<0.001), PW (AUC, 0.999; 95% CI, 0.999 to 1.000, p<0.001) and FSWs (AUC, 1.000; 95% CI, 1.000 to 1.000, p<0.001). The AUC of the urine reagent kits in subjects undergoing VCT was 0.941 (95% CI: 0.876 to 0.978, p<0.001). The ‘acceptable price’ had the greatest influence on STUs (Pi=1.000) and PW (Pi=1.000), the ‘purchase channel’ had the greatest influence on subjects undergoing VCT (Pi=1.000) and persons with IDU (Pi=1.000) and the ‘reagent types’ had the greatest influence on FSWs (Pi=1.000).ConclusionsThe rapid urine test kits showed good diagnostic validity in practical applications, despite a few cases involving misdiagnosis and underdiagnosis.

show abstract

Unveiling Blastocystis epidemiology in Morocco: subtype diversity among clinical patients with and without gastrointestinal manifestations in the Meknes region

Boutahar,

Belaouni,

Ibrahimi

et al. 2024

Parasitol Res

View full text Add to dashboard Cite

Prevalence and genetic characteristics of Blastocystis hominis and Cystoisospora belli in HIV/AIDS patients in Guangxi Zhuang Autonomous Region, China

Cited by 5 publications

References 51 publications

Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones’ culture medium

Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones’ culture medium

Diagnostic performance evaluation of urine HIV-1 antibody rapid test kits in a real-life routine care setting in China

Unveiling Blastocystis epidemiology in Morocco: subtype diversity among clinical patients with and without gastrointestinal manifestations in the Meknes region

Contact Info

Product

Resources

About