Prevalence and genetic characterization of Cryptosporidium species and Giardia duodenalis in lambs in Oromia Special Zone, Central Ethiopia

Wegayehu, Teklu; Karim, Robiul; Li, Junqiang; Adamu, Haileeyesus; Erko, Berhanu; Zhang, Longxian; Tilahun, Getachew

doi:10.1186/s12917-016-0916-0

Cited by 26 publications

(21 citation statements)

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“…Among developing countries, a small number of C. parvum infections have been reported in goats and sheep from Asia, including China [ 17 , 47 , 48 ], India [ 49 ], Jordan [ 50 ] and Turkey [ 51 ]. Our results, however, are in contrast to those from studies conducted in most African countries including Egypt [ 37 ], Tunisia [ 52 ] and Ethiopia [ 53 ], where C. parvum has thus far not been reported in small ruminants. Cryptosporidium parvum was also absent in sheep and goats in other studies in China [ 38 , 41 ].…”

Section: Discussioncontrasting

confidence: 99%

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

et al. 2018

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BackgroundLittle is known on the occurrence and identity of Cryptosporidium species in sheep and goats in Algeria. This study aimed at investigating the occurrence of Cryptosporidium species in lambs and goat kids younger than 4 weeks.MethodsA total of 154 fecal samples (62 from lambs and 92 from kid goats) were collected from 13 sheep flocks in Médea, Algeria and 18 goat flocks across Algiers and Boumerdes. They were screened for Cryptosporidium spp. by nested-PCR analysis of a fragment of the small subunit (SSU) rRNA gene, followed by restriction fragment length polymorphism and sequence analyses to determine the Cryptosporidium species present. Cryptosporidium parvum and C. ubiquitum were further subtyped by sequence analysis of the 60 kDa glycoprotein gene.ResultsCryptosporidium spp. were detected in 17 fecal samples (11.0%): 9 from lambs (14.5%) and 8 from goat kids (8.7%). The species identified included C. parvum in 3 lambs, C. xiaoi in 6 lambs and 6 goat kids, and C. ubiquitum in 2 goat kids. Cryptosporidium infections were detected mostly in animals during the first two weeks of life (7/8 for goat kids and 7/9 for lambs) and in association with diarrhea occurrence (7/17 or 41.2% goat kids and 7/10 or 70.0% lambs with diarrhea were positive for Cryptosporidium spp.). Subtyping of C. parvum and C. ubiquitum isolates identified the zoonotic IIaA13G2R1 and XIIa subtype families, respectively. Minor differences in the SSU rRNA gene sequences were observed between C. xiaoi from sheep and goats.ConclusionsResults of this study indicate that three Cryptosporidium species occur in lambs and goat kids in Algeria, including zoonotic C. parvum and C. ubiquitum. They are associated with the occurrence of neonatal diarrhea.

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Section: Discussioncontrasting

confidence: 99%

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

et al. 2018

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“…The present study also detected C. parvum in sheep and chickens. This is in contrast with many past studies which had demonstrated a rare occurrence of C. parvum in small ruminants and birds in Africa (Robertson et al 2020); with C. xiaoi predominating in sheep and goats in Ghana (Squire et al 2017) and C. ubiquitum predominating in lambs under 5 years in Ethiopia (Wegayehu et al 2017).…”

Section: Discussioncontrasting

confidence: 79%

Genetic Diversity of Cryptosporidium Spp. in Njoro Sub County, Nakuru, Kenya

Essendi

Muleke

Miheso

et al. 2021

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Cryptosporidium spp. cause Cryptosporidiosis in humans through zoonotic and anthroponotic transmission. Previous studies have illustrated the significance of domestic animals as reservoirs of this parasite. However, there is no information on the Cryptosporidium spp. and genotypes circulating in Njoro Sub County. A total of 2174 samples from humans, cattle, chicken, sheep and goats were assessed for presence of Cryptosporidium spp. Thirty-three positive samples were successfully sequenced. The sequences obtained were compared to Cryptosporidium sequences in the GenBank using NCBI’s (National Center for Biotechnology Information) online BLAST (Basic Local Alignment Search Tool) algorithmic program. Sequence alignment was done using the Clustal W program and phylogenetic analysis was executed in MEGA 6 (Molecular Evolutionary Genetics Analysis version 6.0). The Cryptosporidium spp. present in the watershed showed great genetic diversity with nine (9) Cryptosporidium spp. namely: C. parvum, C. hominis, C. ubiquitum, C. meleagridis, C. andersoni, C. baileyi, C. muris, C. xiaoi and C. viatorum. Cattle were the biggest reservoirs of zoonotic Cryptosporidium spp. hence a potential source of zoonosis in humans while goats had the least species. This is the first study that reported presence of C. viatorum in Kenya.

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“…In this investigation, G. duodenalis was detected in both black-boned sheep and goats, with respective prevalences of 21.8% (71/325) and 4.8% (16/336). The prevalence in black-boned sheep was higher than in other kinds of sheep previously reported in China (1.82–13.11%) [ 17 – 24 ], several parts of Australia (20.2%, 11.1%), Spain (11.1%, 19.2%), Ghana (12.9%), Italy (1.5%), Iran (19.8%) and Ethiopia (2.6%) [ 31 , 41 – 47 ], but lower than that in Switzerland (29.8%), Canada (38%), USA (25.4%), Belgium (35.8%), Norway (26.8%), some areas of Australia (63.7%), Spain (42%, 89.2%), Greece (37.3%) and Brazil (34%) [ 48 – 56 ]. As black-boned sheep age, the G. duodenalis prevalence tends to decrease, with a significant difference being observed among different age groups.…”

Section: Discussionmentioning

confidence: 87%

Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China

et al. 2019

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BackgroundGiardia duodenalis is an important intestinal protozoan infecting both humans and animals, causing significant public health concern and immeasurable economic losses to animal husbandry. Sheep and goats have been reported as common reservoirs of G. duodenalis, but only a limited amount of information is available for native breeds of these small ruminants in China. The present study investigated the prevalence and multilocus genotypes of G. duodenalis in black-boned sheep and goats, two important native breeds in Yunnan Province, southwestern China.MethodsFecal samples were collected from 336 black-boned goats and 325 black-boned sheep from five counties (Meishui, Shanshu, Shilin, Yongsheng and Nanping) of Yunnan Province and the genomic DNA was extracted from these feces. The prevalence of G. duodenalis was determined by the nested PCR targeting the β-giardin (bg) gene. The assemblages and multilocus genotypes (MLGs) were investigated based on analyses of three genetic loci, i.e. bg, glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi).ResultsGiardia duodenalis infection was detected in both black-boned sheep and goats, and the prevalence of G. duodenalis in black-boned sheep (21.8%, 71/325) was significantly higher (χ2 = 36.63, df = 1, P < 0.001) than that in black-boned goats (4.8%, 16/336). Significant differences in prevalence were also observed in goats and sheep from different counties (χ2 = 39.83, df = 4, P < 0.001) and age groups (χ2 = 97.33, df = 3, P < 0.001). Zoonotic assemblage A and animal-specific assemblage E were identified in both black-boned sheep and goats with the latter as the predominant assemblage. Based on sequences obtained from the three genetic loci (bg, gdh and tpi), 16 MLGs were obtained in black-boned sheep and goats, including 15 MLGs in assemblage E and one MLG in assemblage A.ConclusionsOur results not only extended the host range of G. duodenalis, but also revealed high genetic variations in G. duodenalis assemblages. The findings of the present study also provide baseline data for preventing and controlling G. duodenalis infection in black-boned sheep and goats in Yunnan Province.

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Prevalence and genetic characterization of Cryptosporidium species and Giardia duodenalis in lambs in Oromia Special Zone, Central Ethiopia

Cited by 26 publications

References 36 publications

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

Genetic Diversity of Cryptosporidium Spp. in Njoro Sub County, Nakuru, Kenya

Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China

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