Prevalence and genetic characterization of Anaplasma marginale in zebu cattle ( Bos indicus ) and their ticks ( Amblyomma variegatum , Rhipicephalus microplus ) from Madagascar

Pothmann, Daniela; Poppert, Sven; Rakotozandrindrainy, Raphäel; Hogan, Benedikt; Mastropaolo, Mariano; Thiel, Claudia; Silaghi, Cornelia

doi:10.1016/j.ttbdis.2016.08.013

Cited by 30 publications

(16 citation statements)

References 36 publications

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“…The overall infection rate with A. marginale described in our study was 11.1%. Several studies detecting A. marginale infection in cattle have been carried out in some regions from Africa with different rates such as in Tunisia (25.4%) (Belkahia et al., ); Morocco (21.9%) (Ait Hamou et al., ); Sudan (6.1%) (Awad et al., ); Egypt (3.7%) (Younis, Hegazy, El‐Deeb, & El‐Khatib, ); Madagascar (89.7%) (Pothmann et al., ); Nigeria (75.9%) (Elelu, Ferrolho, Couto, Domingos, & Eisler, ); Kenya (32.5%) (Adjou Moumouni et al., ); Angola (38%) (Kubelová, Mazancová, & Siroky, ); and Mozambique (67%) (Machado et al., ). This is the case also in Turkey (2.3%) (Aktas et al., ), Brazil (5.4%) (Silva et al., ), Pakistan (17%) (Ashraf et al., ) and Mongolia (8.7%) (Ybañez et al., ).…”

Section: Discussionmentioning

confidence: 99%

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Rjeibi

Ayadi

Rekik³

et al. 2017

Transbound Emerg Dis

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Bovine anaplasmosis could be caused by several Anaplasma species. The causative agents are transmitted by ticks and haematophagous arthropods with a high impact on both human and animal health. This study was conducted to estimate the infection rate and to characterize Anaplasma spp. in cattle from Algeria. A molecular survey was performed in Setif district (Northeast Algeria) where a total number of 180 cattle blood samples were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. PCRs revealed that the infection rates of Anaplasma spp., Anaplasma centrale, Anaplasma marginale and Anaplasma bovis were 42.2%; 39.4%; 11.1% and 4.4%, respectively. All tested animals were negative for A. phagocytophilum. Co-infection occurred in 10% (18/180) of the tested animals, and the most common co-infection pattern was an association between A. centrale and A. marginale (5.5%). Five cattle (2.7%) were co-infected by the three Anaplasma species. Holstein animals (58.1%) were more infected by A. centrale than the other breeds (p = .01). The molecular prevalence of A. centrale was significantly higher in males (54.2%) than in females (34.1%) (p = .001). A. marginale msp4 genetic analysis indicated a high sequence diversity of Algerian strains, suggesting the importation of live cattle from different origins. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. centrale revealed a low degree of genetic diversity. Our study suggests that different species of Anaplasma are simultaneously present in the Algerian cattle. To the best of our knowledge, this is the first molecular study and genetic characterization of Anaplasma spp. in Algerian cattle.

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Section: Discussionmentioning

confidence: 99%

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Rjeibi

Ayadi

Rekik³

et al. 2017

Transbound Emerg Dis

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“…A semi-nested PCR targeting the msp1α gene was carried out on 19 DNA samples from cattle and buffaloes that tested positive for A. marginale by qPCR, registering CT values ≤ 25 cycles [11,16] (Table 1). The first PCR was done on both cattle and buffalo samples.…”

Section: Genotyping Of Anaplasma Marginalementioning

confidence: 99%

“…Clear strong specific bands (size ranging from 800 to 1000 bp) were obtained from cattle samples while buffalo samples were subjected to the second amplification. The PCR reactions were performed using GoTaq ® Flexi DNA Polymerase kit (Promega, Madison, USA) as previously described [11,16,17,25,28].…”

Section: Genotyping Of Anaplasma Marginalementioning

confidence: 99%

“…Bovine anaplasmosis is characterized by mild to severe hemolysis and anemia that adversely affects animal health, production and reproduction [9,10]. It is caused by Anaplasma marginale, an intraerythrocytic rickettsia mainly transmitted by Rhipicephalus microplus ticks, but other tick species have also been incriminated as vectors worldwide [11,12]. Mechanical transmission through contaminated needles or surgical instruments under poor hygienic conditions or through biting flies may also occur.…”

Section: Introductionmentioning

confidence: 99%

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Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt

et al. 2020

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Background Anaplasma marginale is an obligate intracellular bacterium and the main cause of bovine anaplasmosis in tropical and subtropical regions. In Egypt, data regarding the prevalence of A. marginale in ruminant hosts and of the circulating genotypes is lacking. This study therefore aimed to (i) investigate the presence, epidemiology and genotypes of A. marginale in cattle and buffaloes in Egypt, (ii) to evaluate suitable diagnostic tools and (iii) to identify co-infections of A. marginale with other selected tick-borne pathogens. Methods Blood samples were collected from 394 animals (309 cattle and 85 buffaloes) from three different areas in Egypt. For the detection of A. marginale infection, several tests were compared for their sensitivity and specificity: blood smear analysis, enzyme-linked immunosorbent assay (ELISA), PCR, real-time PCR and reverse line blot (RLB) assay. Co-infections with A. marginale, piroplasms and other Anaplasmataceae were surveyed by RLB while A. marginale genotypes were identified by amplifying and sequencing the partial msp1α gene. Results Anaplasma marginale DNA was amplified by qPCR in 68.3% of cattle and 29.4% of buffaloes. RLB showed infection with A. marginale in 50.2% of cattle and 42.5% of buffaloes. Blood smear analysis detected this agent in 16.2% of cattle and 2.4% of buffaloes. ELISA showed specific antibodies against A. marginale in 54.9% of cattle. Anaplasma marginale was associated, in cattle and buffaloes, with several tick-borne pathogens (Theileria annulata, Babesia bovis, Babesia bigemina, Babesia occultans and Anaplasma platys). A significant difference of A. marginale infection level was noticed in cattle, where animals between 3–5-years-old had a higher prevalence (79.2%) compared to those older than 5 years (36.4%) and younger than 3 years (59.7%) and one year (64.5%), respectively (P = 0.002281). Microsatellite analysis identified 15 different genotypes. Conclusions The epidemiological findings revealed high prevalence of A. marginale in cattle and buffaloes in all the investigated areas. The circulation of diverse genotypes was observed, most of these A. marginale genotypes being specific for Egypt. The qPCR assay was confirmed to be the most sensitive tool for detection of A. marginale in cattle and buffaloes even in the carrier state, highlighting the importance of using suitable diagnostic tests.

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“…In earlier studies, Pong et al (2012) reported 87% and 60% prevalence of anaplasmosis in Peninsular and East Malaysian cattle, respectively; revealing its endemicity in Malaysian cattle. The current seroprevalence rate of Anaplasma in most small holder cattle farms in Peninsular Malaysia is unknown and this could be a serious threat to cattle health and production in this area.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 90%

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

Bitrus¹,

Abba²,

Jesse³

et al. 2018

Adv. Anim. Vet. Sci.

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Prevalence and genetic characterization of Anaplasma marginale in zebu cattle ( Bos indicus ) and their ticks ( Amblyomma variegatum , Rhipicephalus microplus ) from Madagascar

Cited by 30 publications

References 36 publications

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

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