Prevalence and Genetic Diversity of Grapevine Virus D in Tunisia

Selmi, Ilhem; Elbeaino, Toufic; Lehad, A.; Air, Manel El; Digiaro, M.; Mahfoudhi, N.

doi:10.52543/tjpp.16.2.1

Cited by 2 publications

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“…Phylogenetic typing confirmed the prevalence of molecular group IV in the vineyards of southern Russia, whereas group I was less prevalent. For comparison, the absolute majority of isolates in Tunisia (70%) belonged to group I, while group IV was the second most prevalent [35]. The prevalence of group I was also reported in Turkey, Iran, and Slovakia [36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Distribution and Genetic Diversity of Grapevine Viruses in Russia

Porotikova

Terehova

Volodin

et al. 2021

Plants

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Viral diseases can seriously damage the vineyard productivity and the quality of grape and wine products. Therefore, the study of the species composition and range of grapevine viruses is important for the development and implementation of strategies and tactics to limit their spread and increase the economic benefits of viticulture. In 2014–2019, we carried out a large-scale phytosanitary monitoring of Russian commercial vineyards in the Krasnodar region, Stavropol region and Republic of Crimea. A total of 1857 samples were collected and tested for the presence of Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine fanleaf virus (GFLV), and Grapevine fleck virus (GFkV) using RT-PCR. Out of all samples tested, 54.5% were positive for at least one of the viruses (GRSPaV, GVA, GLRaV-1, GLRaV-2, GLRaV-3, GFLV, GFkV) in the Stavropol region, 49.8% in the Krasnodar region and 49.5% in the Republic of Crimea. Some plants were found to be infected with several viruses simultaneously. In the Republic of Crimea, for instance, a number of plants were infected with five viruses. In the Krasnodar region and the Republic of Crimea, 4.7% and 3.3% of the samples were predominantly infected with both GFkV and GRSPaV, whereas in the Stavropol region, 6% of the selected samples had both GLRaV-1 and GVA infections. We carried out a phylogenetic analysis of the coat protein genes of the detected viruses and identified the presence of GVA of groups I and IV, GRSPaV of groups BS and SG1, GLRaV-1 of group III, GLRaV-2 of groups PN and H4, GLRaV-3 of groups I and III. The results obtained make it possible to assess the viral load and the distribution of the main grapevine viruses on plantations in the viticultural zones of Russia, emphasizing the urgent need to develop and implement long-term strategies for the control of viral diseases of grapes.

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Section: Discussionmentioning

confidence: 99%

Distribution and Genetic Diversity of Grapevine Viruses in Russia

Porotikova

Terehova

Volodin

et al. 2021

Plants

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“…The closest were isolates from South Africa. This molecular group is the most widespread in Slovakia, Turkey, Iran, and Tunisia [83][84][85][86]. Moreover, when we examined the coat protein gene of isolates detected in our previous studies in the Krasnodar Krai and the Republic of Crimea, we also identified isolates of GVA molecular groups II and IV [34,35].…”

Section: Family: Betaflexiviridaementioning

confidence: 99%

Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Shvets

Porotikova

Sandomirsky

et al. 2022

Viruses

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Grapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grapevine germplasm collection, the Anapa Ampelographic Collection, using high-throughput sequencing of total RNAs. As a result of bioinformatics analysis and validation of its results by reverse transcription PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR), we identified 20 viruses and 3 viroids in 47 libraries. All samples were infected with 2 to 12 viruses and viroids, including those that cause economically significant diseases: leafroll, fleck, and rugose wood complex. For the first time in Russia, we detected Grapevine virus B (GVB), Grapevine virus F (GVF), Grapevine asteroid mosaic-associated virus (GAMaV), Grapevine Red Globe virus (GRGV), Grapevine satellite virus (GV-Sat), Grapevine virga-like virus (GVLV), Grapevine-associated jivivirus 1 (GaJV-1) and Vitis cryptic virus (VCV). A new putative representative of the genus Umbravirus with the provisional name Grapevine umbra-like virus (GULV) was also identified in Russian grape samples.

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Prevalence and Genetic Diversity of Grapevine Virus D in Tunisia

Cited by 2 publications

References 0 publications

Distribution and Genetic Diversity of Grapevine Viruses in Russia

Distribution and Genetic Diversity of Grapevine Viruses in Russia

Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

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