Prevalence and genetic diversity of Bartonella species in sika deer (Cervus nippon) in Japan

Sato, Shingo; Kabeya, Hidenori; Yamazaki, Mari; Takeno, Shinako; Suzuki, Kazuo; Kobayashi, Shinichi; Souma, Kousaku; Masuko, Takayoshi; Chomel, Bruno B; Maruyama, Soichi

doi:10.1016/j.cimid.2012.07.001

Cited by 18 publications

(35 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sika Deer WD12.1Sika deer ( Cervus nippon )21,739,423/49,5532/137.9/34.91,565/71sequenced(Sato et al 2012b)/this study B. sp. Sika Deer WD16.2Sika deer ( Cervus nippon )21,762,996137.61,526sequenced(Sato et al 2012b)/this study B. sp. CDC_skunkStripped skunk ( Mephitis mephitis )31,615,223136.11,374sequencedthis study/this study B. sp.…”

Section: Methodsmentioning

confidence: 85%

Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella

Harms

Segers

Québatte

et al. 2017

Genome Biology and Evolution

Self Cite

View full text Add to dashboard Cite

The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex effector repertoires. However, the limited availability of genome sequences from one of those lineages as well as other, remote branches of Bartonella has so far hampered comprehensive understanding of how the VirB/D4 T4SS and its effectors called Beps have shaped Bartonella evolution. Here, we report the discovery of a third repertoire of Beps associated with the VirB/D4 T4SS of B. ancashensis, a novel human pathogen that lacks any signs of host adaptability and is only distantly related to the two species-rich lineages encoding a VirB/D4 T4SS. Furthermore, sequencing of ten new Bartonella isolates from under-sampled lineages enabled combined in silico analyses and wet lab experiments that suggest several parallel layers of functional diversification during evolution of the three Bep repertoires from a single ancestral effector. Our analyses show that the Beps of B. ancashensis share many features with the two other repertoires, but may represent a more ancestral state that has not yet unleashed the adaptive potential of such an effector set. We anticipate that the effectors of B. ancashensis will enable future studies to dissect the evolutionary history of Bartonella effectors and help unraveling the evolutionary forces underlying bacterial host adaptation.

show abstract

Section: Methodsmentioning

confidence: 85%

Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella

Harms

Segers

Québatte

et al. 2017

Genome Biology and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, DNA of B. schoenbuchensis was detected in samples from European deer keds in France (Halos et al., ), and also found in both European (Matsumoto et al., ) and neotropical deer keds (Lipoptena mazamae) in the USA (Reeves et al., ). Therefore, these ectoparasites (deer ked) are considered as possible vectors that infect deer (Sato et al., ). Bartonella schoenbuchensis ‐related species were identified in spleen tissues of wild Korean water deer (Ko et al., ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Survey ofAnaplasmaandEhrlichiaof Red Deer and Sika Deer in Gansu, China in 2013

Chen

Liu

et al. 2015

Transbound Emerg Dis

View full text Add to dashboard Cite

Anaplasma and Ehrlichia are important emerging tick-borne pathogens in both humans and animals. Here, we conducted a molecular surveillance study in Gansu, China to assess the prevalence of Anaplasma and Ehrlichia spp. in red deer and sika deer based on polymerase chain reaction (PCR) analysis and sequencing of 16S rRNA or msp genes. PCR revealed that the prevalence of Anaplasma ovis, Anaplasma bovis and Anaplasma platys of the Qilian Mountain samples was 32%, 9% and 9%, respectively; the prevalence of Anaplasma ovis, Anaplasma bovis, Anaplasma platys was 20%, 15% and 15% among the Long Mountain samples, respectively. Of the Long Mountain samples, two (5%) of the 40 samples were positive for Ehrlichia canis, but all 44 of the Qilian Mountain samples were negative for E. canis, and no other Anaplasma or Ehrlichia spp. were found in the samples. The phylogenetic tree showed that the newly isolated Anaplasma and Ehrlichia spp. could be classified as belonging to four clades, including an A. bovis cluster, A. ovis cluster, A. platys cluster and E. canis cluster. In addition, Bartonella schoenbuchensis was firstly identified in blood samples from red deer in Gansu, China. Our results provide important data to increase the understanding of the epidemiology of anaplasmosis and ehrlichiosis of red deer and sika deer and will assist with the implementation of measures to control anaplasmosis and ehrlichiosis transmission to red deer, sika deer and other animals in Gansu, China.

show abstract

“…13 nippon), which are reported to harbor several Bartonella species with infection rates as high as 41.5% (Sato et al, 2012). Octadecabacter species, on the other hand, are psychrophilic marine microorganisms collected from polar areas.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Taxonomic profiles in metagenomic analyses of free-living microbial communities in the Ofunato Bay

Reza

Kobiyama

Yamada

et al. 2018

Gene

View full text Add to dashboard Cite

The Ofunato Bay in Iwate Prefecture, Japan is a deep coastal bay located at the center of the Sanriku Rias Coast and considered an economically and environmentally important asset. Here, we describe the first whole genome sequencing (WGS) study on the microbial community of the bay, where surface water samples were collected from three stations along its length to cover the entire bay; we preliminarily sequenced a 0.2 μm filter fraction among sequentially size-fractionated samples of 20.0, 5.0, 0.8 and 0.2 μm filters, targeting the free-living fraction only. From the 0.27-0.34 Gb WGS library, 0.9 × 10-1.2 × 10 reads from three sampling stations revealed 29 bacterial phyla (~80% of assigned reads), 3 archaeal phyla (~4%) and 59 eukaryotic phyla (~15%). Microbial diversity obtained from the WGS approach was compared with 16S rRNA gene results by mining WGS metagenomes, and we found similar estimates. The most frequently recovered bacterial sequences were Proteobacteria, predominantly comprised of 18.0-19.6% Planktomarina (Family Rhodobacteraceae) and 13.7-17.5% Candidatus Pelagibacter (Family Pelagibacterales). Other dominant bacterial genera, including Polaribacter (3.5-6.1%), Flavobacterium (1.8-2.6%), Sphingobacterium (1.4-1.6%) and Cellulophaga (1.4-2.0%), were members of Bacteroidetes and likely associated with the degradation and turnover of organic matter. The Marine Group I Archaea Nitrosopumilus was also detected. Remarkably, eukaryotic green alga Bathycoccus, Ostreococcus and Micromonas accounted for 8.8-15.2%, 3.6-4.9% and 2.1-3.1% of total read counts, respectively, highlighting their potential roles in the phytoplankton bloom after winter mixing.

show abstract

Prevalence and genetic diversity of Bartonella species in sika deer (Cervus nippon) in Japan

Cited by 18 publications

References 33 publications

Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella

Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella

Molecular Survey ofAnaplasmaandEhrlichiaof Red Deer and Sika Deer in Gansu, China in 2013

Taxonomic profiles in metagenomic analyses of free-living microbial communities in the Ofunato Bay

Contact Info

Product

Resources

About