Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada

Appelbee, A.J.; Frederick, Lindsey M.; Heitman, T. Lee-Ann; Olson, Merle E.

doi:10.1016/s0304-4017(02)00422-3

Cited by 222 publications

(144 citation statements)

References 14 publications

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“…Previous data about Giardia genotypes in calves from Western Australia and Western Canada (O'Handley et al, 2000) showed dairy calves to be infected with two Assemblages, A and E. Moreover, genotypic analysis of Giardia isolates from beef cattle from Alberta (Canada) showed that the hoofed livestock genotype is predominant, but the zoonotic genotype, Assemblage A, is reported to be as high as 20% (Appelbee et al, 2003). Genetic analysis of Giardia isolates from calves examined in this study showed only the presence of Assemblage E. The animals resulted also positive for Eimeria sp.…”

Section: Discussionmentioning

confidence: 99%

Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Berrilli

Cave

Liberato

et al. 2004

Veterinary Parasitology

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In order to investigate the genotypes of Giardia duodenalis from domestic and farm animals in Italy, 21 Giardia isolates, 17 from dogs, 1 from cat and 3 from dairy calves, were genetically characterised by SSU-rRNA gene sequencing. Among dogs, 76.5% of isolates showed the dog-specific genotypes (Assemblages C, D and C/D mixed Assemblage) and 23.5% exhibit potential zoonotic genotypes (Assemblage A and A/C mixed Assemblages). The cat isolate belonged to assemblage A, whereas the sequences among the isolates from calves were found to correspond to hoofed-livestock genotype, namely Assemblage E. These findings suggest that infection of humans by zoonotic genotypes from domestic animals could be of low epidemiological significance, although possible. The present study represents the first contribute to the knowledge of G. duodenalis genotypes in domestic and farm animals from Italy.

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Section: Discussionmentioning

confidence: 99%

Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Berrilli

Cave

Liberato

et al. 2004

Veterinary Parasitology

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“…Studies in the United States, Canada, Australia and Japan suggest that the public health risk from cattle is minimal , although the zoonotic Giardia duodenalis assemblage A has been reported in up to 20% of either pre-weaned calves (O'Handley et al, 2000;Appelbee et al, 2003;Becher et al, 2004;Trout et al, 2004;Itagaki et al, 2005), post-weaned calves (Trout et al, 2005) or adult cattle (Trout et al, 2006;Uehlinger et al, 2006). Occasionally, G. duodenalis assemblage B has been reported in calves (van Keulen et al, 2002;Lalle et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Mixed Giardia duodenalis assemblage A and E infections in calves

Geurden

Geldhof

Levecke

et al. 2008

International Journal for Parasitology

153

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A molecular epidemiological study was conducted on 100 dairy (499 calves) and 50 beef (333 calves) farms in Belgium to estimate the prevalence of different Giardia duodenalis assemblages in calves younger than 10 weeks of age. Positive samples from the epidemiological study and from a previous clinical study were selected and genotyped based on the amplification of the b-giardin gene. To investigate the occurence of mixed assemblage A and E infections in calves, a novel assemblage-specific PCR was developed based on the triose-phosphate isomerase gene. The prevalence was 22% (95% Probability Interval (PI): 12-34%) in dairy calves and 45% (95% PI: 30-64%) in beef calves. In total, 120 Giardia-positive samples from dairy and beef calves collected in the epidemiological study and from clinically affected calves were identified based on the amplification of the b-giardin gene. Overall G. duodenalis assemblage E was more prevalent (in 64% of the samples), although the majority (59%) of the dairy calves were infected with G. duodenalis assemblage A. Furthermore, mixed G. duodenalis assemblage A and E infections were identified in 31% of the calf samples (n = 101) using the assemblage-specific PCR. We believe this is the first report of mixed infections in calves, and the results of the present study indicate that calves, although mainly infected with the host-specific G. duodenalis assemblage E, are frequently infected with the zoonotic assemblage A, either as a mixed or mono-infection, suggesting that calves might be underestimated as a potential zoonotic reservoir for human infections. Ó

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“…encontrada en este estudio concuerda con los datos reportados en otras partes (2,12,(14)(15)(16)(17)23). Anotando que los animales positivos (33 %, n=115) presentaron diarrea en el momento de la toma de la muestra.…”

Section: Discusionunclassified

Prevalencia y factores de riesgo de Cryptosporidium spp. y Giardia spp. en terneros de ganado lechero de la zona noroccidental de la Sabana de Bogotá

Hernández-Gallo

Cortés-Vecino

2012

Rev. salud pública

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Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada

Cited by 222 publications

References 14 publications

Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Mixed Giardia duodenalis assemblage A and E infections in calves

Prevalencia y factores de riesgo de Cryptosporidium spp. y Giardia spp. en terneros de ganado lechero de la zona noroccidental de la Sabana de Bogotá

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