Prevalence and geographical distribution of <i>Nosema apis</i> and <i>Nosema ceranae</i> in apiaries of Northwest Mexico using a duplex real-time PCR with melting-curve analysis

González, Sergio Arturo Cueto; López-Valencia, Gilberto; Cabrera, Carolina Orozco; Gómez-Gómez, Sergio Daniel; Torres, Kattya Moreno; Blandón, Kelvin Orlando Espinoza; Velázquez, José Guadalupe Guerrero; Paz, Laura Elena Silva; Muñoz, Enrique Trasviña; Monge-Navarro, Francisco Javier

doi:10.1080/00218839.2019.1676999

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Other humid and colony-crowded areas of Mexico, such as the coastal regions, followed the Yucatan Peninsula in the frequency of nosema disease. The least-colony-crowded and humid regions (North and High Plateau) had the lowest frequencies of the disease, although a recent study in the state of Baja California (Northwest of Mexico) diagnosed nosemosis by RT-PCR, finding a disease prevalence >50% [61]. In our study, the samples were not analyzed for Nosema species, something that should be considered in future surveys.…”

Section: Discussionmentioning

confidence: 83%

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Correa-Benítez,

Anguiano-Baez,

Heneidi-Zeckua

et al. 2023

Animals

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Mexico is a major honey producer, but not much information exists about the health status of honey bees (Apis mellifera L.) in the country. This study was conducted to determine the sanitary status of adult honey bees in Mexico’s five beekeeping regions. Samples from 369 apiaries were diagnosed to identify pathogens such as Varroa destructor, which was quantified, Acarapis woodi, Nosema spp., and five viruses. Colonies were also inspected for the presence of the small hive beetle (SHB), Aethina tumida. Varroa destructor was found in 83.5% of the apiaries, with the Pacific Coast region having the highest prevalence (>95%) and rates (4.5% ± 0.6). Acarapis woodi was detected in only one apiary from the Pacific Coast, whereas Nosema spp. were prevalent in 48.5% of the apiaries, with the highest and lowest frequencies in the Yucatan Peninsula and North regions (64.6% and 10.2%, respectively). For viruses, deformed wing virus (DWV) was detected in 26.1% of the apiaries, with the highest frequency in the Pacific Coast region (44.7%). Israeli acute paralysis virus (IAPV) was diagnosed in 3.2% of the samples and sacbrood bee virus (SBV) in 23.3% of them, with the highest frequency in the High Plateau region (36.4%). Chronic bee paralysis and Kashmir bee viruses were not detected. SHB prevalence was 25.2% nationwide, with the highest frequency in the Yucatan Peninsula (39.2%). This study shows that the most common parasites of adult honey bees in Mexico are V. destructor and Nosema spp., and that the most prevalent virus is DWV, whereas SHB is highly prevalent in the Yucatan Peninsula. This information could be useful to design disease control strategies for honey bee colonies in different regions of Mexico.

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Section: Discussionmentioning

confidence: 83%

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Correa-Benítez,

Anguiano-Baez,

Heneidi-Zeckua

et al. 2023

Animals

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“…Es recomendable mantener los muestreos periódicos para una correcta vigilancia y la identificación de las especies circulantes, ya que la nosemosis puede ser causada por N. apis que se considera de baja virulencia, pero también puede ser causada por N. ceranea (nosemosis tipo C) con una mayor virulencia y que ha desplazado a N. apis como la causa principal de nosemosis en las abejas melíferas, lo que podría poner en riesgo la supervivencia de A. mellifera en Honduras (Fleites-Ayil et al, 2018;González et al, 2020).…”

Section: Discussionunclassified

Varroasis y nosemosis en colmenas de Apis mellifera en el municipio de Tomalá, Honduras

Pineda Nataren,

Videz,

Flores Somarriba

2024

Agron. Mesoam.

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Introducción. La apicultura en Honduras es una actividad importante para el sector pecuario, amenazada por la presencia de plagas y enfermedades que afectan el desarrollo y la producción de las colmenas. Objetivo. Determinar la carga parasitaria de Varroa destructor y Vairimorpha (Nosema) spp asociadas al comportamiento higiénico, productividad y presencia de otras plagas en colmenas de Apis mellifera en el municipio de Tomalá, departamento de Lempira, Honduras. Materiales y métodos. Se realizó un estudio descriptivo de corte transversal en 57 muestras de colmenas de 19 apiarios, analizadas con el método de tamiz para determinar la tasa de infestación de Varroa destructor. Para encontrar la tasa media de infección de esporas de Vairimorpha spp se utilizó el método de Cantwell. Además, se determinó la asociación entre las cargas parasitarias y las variables como productividad, comportamiento higiénico y presencia de otras plagas, se aplicó un análisis estratificado de acuerdo con el antecedente del uso o no de tratamiento contra Varroa spp. Los datos se analizaron a través de medidas de tendencia central y prueba de Shapiro Wilk. La determinación de asociaciones se realizó mediante las pruebas no paramétricas U de Mann Whitney y Kruskal Wallis. Resultados. Las tasas de infestación para V. destructor en fase de dispersión y larval fueron de un 3,48 % y un 6,82 %, respectivamente, el antecedente de aplicación de tratamiento químico no mostró asociación con la tasa de infestación de V. destructor en larvas ni en adultos (p≥0,05), la presencia del pequeño escarabajo de las colmenas (PEC), se asoció con una mayor infestación de V. destructor en larvas (p˂0,007) en las colmenas que no habían recibido tratamiento. El rango de infección de Vairimorpha spp se encontró dentro de la clasificación muy ligera (IC 50 000,00-260 000,00) con 50 000,00 esporas por muestra, la carga de Vairimorpha spp no mostró asociación con las variables mencionadas (p≥0,05). Conclusión. Los resultados indican que varroasis es una enfermedad de importancia en el municipio de Tomalá, departamento de Lempira, Honduras, asociada con el comportamiento higiénico y la presencia de PEC en las colmenas. Nosemosis presentó una infección muy ligera.

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“…Conversely, only N. ceranae was found in Saudi Arabia [ 41 ]. Finally, a study from Mexico showed that N. apis was dominant (87%) in an area with a warm climate [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Variation in the Distribution of Nosema Species in Honeybees (Apis mellifera Linnaeus) between the Neighboring Countries Estonia and Latvia

Naudi

Šteiselis²,

Jürison

et al. 2021

Veterinary Sciences

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The unicellular spore-forming parasites Nosema apis and Nosema ceranae are considered to be one of the causes of increased honey bee mortality in recent years. These pathogens attack their honey bee hosts through their gut, causing changes in behavioral stress responses and possibly resulting in decreased honey yield and increased honey bee mortality. The present study aimed to determine the prevalence of Nosema spp. (nosemosis) in Estonia and Latvia, as well as the persistence of the disease in previously infected hives. Currently, N. ceranae is considered the most virulent species and is predominant worldwide. However, in some regions, usually with colder climates, N. apis is still prevalent. To achieve better disease control, it is important to determine the species distribution. For this purpose, we selected 30 apiaries in Estonia and 60 in Latvia that were positive for Nosema spp. in the EPILOBEE (2012–2014) study, which was 5 years prior to the present study. The results show that, while both species are present in Estonia and Latvia, N. apis is dominant in Estonia (43%), and N. ceranae is dominant in Latvia (47%). We also found that the pathogens are very persistent, since 5 years later, only 33% of infected apiaries in Estonia and 20% of infected apiaries in Latvia, we could not detect any pathogens at the time of sampling.

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Prevalence and geographical distribution of Nosema apis and Nosema ceranae in apiaries of Northwest Mexico using a duplex real-time PCR with melting-curve analysis

Cited by 8 publications

References 41 publications

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Varroasis y nosemosis en colmenas de Apis mellifera en el municipio de Tomalá, Honduras

Variation in the Distribution of Nosema Species in Honeybees (Apis mellifera Linnaeus) between the Neighboring Countries Estonia and Latvia

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