“…This study, whose objective was to investigate the prevalence of DF and its severity, is the first carried out in the southern region of the country, in provinces with different climatic and altitude characteristics such as Azuay, Cañar and Morona Santiago, which are apparently non-fluoridated areas, so the data obtained can serve as a starting point for future research. The results show a high prevalence of DF in the three provinces evaluated, with no significant differences between them ( p > 0.05), coinciding with previous studies carried out in some Latin American cities [ 31 , 35 , 36 , 37 , 38 , 39 ] and in Ecuador [ 30 , 40 , 41 , 42 ] ( Figure 2 ). The fluoride incorporated into the organism from water sources, processed or not processed food, or by accidental ingestion, through the consumption of toothpaste, constitutes the main source for pathologies at the dental enamel level [ 43 ], with an effect variable according to the period of contact of the mineral with the tooth in its formation stage [ 44 ].…”