Prevalence and Mechanism of Atrazine Resistance in Waterhemp (<i>Amaranthus tuberculatus</i>) from Nebraska

Vennapusa, Amaranatha R.; Faleco, Felipe A.; Vieira, Bruno C.; Samuelson, Spencer; Kruger, Greg R.; Werle, Rodrigo; Jugulam, Mithila

doi:10.1017/wsc.2018.38

Cited by 26 publications

(33 citation statements)

References 54 publications

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“…Enhanced herbicide metabolism via P450 enzymes was also reported in a waterhemp population resistant to HPPD-inhibitor herbicides in Nebraska 69,70 . Atrazine resistance with rapid herbicide metabolism via enhanced GST conjugation was widespread in waterhemp populations in Nebraska 54 . Although non-target-site glyphosate resistance with metabolism in plants is relatively rare 71 , non-target-site resistance with reduced glyphosate translocation was identified in waterhemp biotypes in Mississippi 72 .…”

Section: Populationmentioning

confidence: 99%

“…Both are obligate outcrossing dioecious weed species with a fast growth habitat, extended emergence window, and prolific seed production with high genetic plasticity which pose a challenge to their management 37-44 . Numerous Palmer amaranth and waterhemp populations have evolved resistance to herbicides that target 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), 4-hydroxyphenylpyruvate dioxygenase (HPPD), photosystem II, protoporphyrinogen oxidase (PPO), auxin receptors, microtubule assembly, and acetolacte synthase (ALS) in the US 15,17,[45][46][47][48][49][50][51][52][53][54] . Moreover, pollen mediated gene flow has been reported as a major contributor to herbicide resistance dissemination in Palmer amaranth and waterhemp in the US Midwest 55,56 .Although controlling weed populations on field margins and ditches is considered a best management practice to delay herbicide resistance evolution, these weed populations are often neglected in agricultural landscapes [15][16][17]29 .…”

mentioning

confidence: 99%

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Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Vieira

Luck

Amundsen

et al. 2020

Sci Rep

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While the introduction of herbicide tolerant crops provided growers new options to manage weeds, the widespread adoption of these herbicides increased the risk for herbicide spray drift to surrounding vegetation. the impact of herbicide drift in sensitive crops is extensively investigated, whereas scarce information is available on the consequences of herbicide drift in non-target plants. Weeds are often abundant in field margins and ditches surrounding agricultural landscapes. Repeated herbicide drift exposure to weeds could be detrimental to long-term management as numerous weeds evolved herbicide resistance following recurrent-selection with low herbicide rates. the objective of this study was to evaluate if glyphosate, 2,4-D, and dicamba spray drift could select Amaranthus spp. biotypes with reduced herbicide sensitivity. palmer amaranth and waterhemp populations were recurrently exposed to herbicide drift in a wind tunnel study over two generations. Seeds from survival plants were used for the subsequent rounds of herbicide drift exposure. progenies were subjected to herbicide doseresponse studies following drift selection. Herbicide drift exposure rapidly selected for Amaranthus spp. biotypes with reduced herbicide sensitivity over two generations. Weed management programs should consider strategies to mitigate near-field spray drift and suppress the establishment of resistance-prone weeds on field borders and ditches in agricultural landscapes.Some researchers also suggest that low rates of herbicides could induce new stress-related mutations and epigenetic alterations on weeds, ultimately leading to reduced herbicide sensitivity [33][34][35] .Recombination and accumulation of minor resistance alleles can occur at a faster rate in cross-pollinated species, such as Palmer amaranth and waterhemp, during recurrent selection with low rates of herbicides 21,22,36 . These C4 summer annual Amaranthus spp. are among the most troublesome weed species in the US row crop production systems 37 . Both are obligate outcrossing dioecious weed species with a fast growth habitat, extended emergence window, and prolific seed production with high genetic plasticity which pose a challenge to their management 37-44 . Numerous Palmer amaranth and waterhemp populations have evolved resistance to herbicides that target 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), 4-hydroxyphenylpyruvate dioxygenase (HPPD), photosystem II, protoporphyrinogen oxidase (PPO), auxin receptors, microtubule assembly, and acetolacte synthase (ALS) in the US 15,17,[45][46][47][48][49][50][51][52][53][54] . Moreover, pollen mediated gene flow has been reported as a major contributor to herbicide resistance dissemination in Palmer amaranth and waterhemp in the US Midwest 55,56 .Although controlling weed populations on field margins and ditches is considered a best management practice to delay herbicide resistance evolution, these weed populations are often neglected in agricultural landscapes [15][16][17]29 . The hypothesis of this study is that rep...

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Section: Populationmentioning

confidence: 99%

mentioning

confidence: 99%

Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Vieira

Luck

Amundsen

et al. 2020

Sci Rep

Self Cite

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“…NTSR to PS-II inhibitors have been documented in annual bluegrass [63], common ragweed [64], common waterhemp [65,66], Palmer amaranth [67,68], and wild radish [62]. In the majority of these cases, the metabolism of PS-II inhibitors was catalyzed by the enhanced activity of GST enzymes [69] and/or CYP450 enzymes [70].…”

Section: Known Ntsr Mechanisms In Weed Species For Different Herbimentioning

confidence: 99%

“…For example, atrazine-resistant Palmer amaranth from Kansas was found to conjugate atrazine 24 times faster than the susceptible plants via enhanced GST-activity [67]. Similarly, the enhanced metabolism of atrazine was found in two common waterhemp populations from Illinois [10] and Nebraska [65]. In the atrazine-resistant common waterhemp from Nebraska, at 6 HAT, approximately 92% of the atrazine was found to be conjugated by GSTs, whereas 92% of atrazine was still retained as a parent compound in susceptible plants [65].…”

Section: Known Ntsr Mechanisms In Weed Species For Different Herbimentioning

confidence: 99%

Non-Target-Site Resistance to Herbicides: Recent Developments

Jugulam

Shyam

2019

Plants

132

114

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Non-target-site resistance (NTSR) to herbicides in weeds can be conferred as a result of the alteration of one or more physiological processes, including herbicide absorption, translocation, sequestration, and metabolism. The mechanisms of NTSR are generally more complex to decipher than target-site resistance (TSR) and can impart cross-resistance to herbicides with different modes of action. Metabolism-based NTSR has been reported in many agriculturally important weeds, although reduced translocation and sequestration of herbicides has also been found in some weeds. This review focuses on summarizing the recent advances in our understanding of the physiological, biochemical, and molecular basis of NTSR mechanisms found in weed species. Further, the importance of examining the co-existence of TSR and NTSR for the same herbicide in the same weed species and influence of environmental conditions in the altering and selection of NTSR is also discussed. Knowledge of the prevalence of NTSR mechanisms and co-existing TSR and NTSR in weeds is crucial for designing sustainable weed management strategies to discourage the further evolution and selection of herbicide resistance in weeds.

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“…The ED 50 value for the NER biotype was higher (P < 0.05) than that of the susceptible biotype (S1) (data not shown), and based on the ED 50 values, the NER biotype was 7-fold resistant to atrazine compared with the S1 biotype. Atrazine-resistant A. tuberculatus in Nebraska was first reported in 1990 (Anderson et al 1996), and A. tuberculatus resistant to atrazine applied POST is widespread in Nebraska: a 2014 survey of 85 fields in eastern Nebraska revealed that atrazine-resistant A. tuberculatus was present in 73% of all fields surveyed (Vennapusa et al 2018). The occurrence of atrazine-resistant A. tuberculatus with a high level of resistance is also widespread in other states in the Midwest (Heap 2019b): for example, a recent report suggested that a multiple herbicide-resistant biotype from Missouri showed 7-to 19-fold resistance to atrazine (Shergill et al 2018a).…”

Section: Regression Parameters (±Sem)mentioning

confidence: 99%

Protoporphyrinogen oxidase (PPO) inhibitor–resistant waterhemp (Amaranthus tuberculatus) from Nebraska is multiple herbicide resistant: confirmation, mechanism of resistance, and management

et al. 2019

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A waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] biotype (designated as “NER”) collected from a soybean [Glycine max (L.) Merr.] production field in eastern Nebraska survived the POST application of fomesafen at the labeled rate. The objectives of this study were to (1) quantify the level of resistance to protoporphyrinogen oxidase (PPO) inhibitors (acifluorfen, fomesafen, and lactofen) applied POST, (2) determine the mechanism of PPO-inhibitor resistance in the NER biotype, (3) determine whether NER possessed multiple resistance to acetolactate synthase (ALS)-, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-, and photosystem II (PSII)-inhibiting herbicides, and (4) control NER with POST soybean herbicides. A whole-plant dose–response bioassay revealed that the NER biotype was 4- to 6-fold resistant to PPO-inhibiting herbicides depending on the known susceptible biotype (S1 or S2) used for comparison. A Kompetitive Allele Specific PCR (KASP™) assay was developed and performed for rapid and robust detection of the ΔG210 mutation (deletion of a codon) in the PPX2L gene. All samples of the NER biotype tested positive for the ΔG210 mutation. Dose–response bioassays confirmed that the NER biotype was resistant to three additional herbicide sites of action. Chlorimuron and imazethapyr, both ALS inhibitors, applied at 32X the labeled rate resulted in <80% reduction in the aboveground biomass of the NER biotype. The same biotype was 3- and 7-fold resistant to glyphosate (EPSPS inhibitor) and atrazine (PSII inhibitor), respectively. Glufosinate, 2,4-D choline plus glyphosate, and dicamba were the only soybean POST herbicides that controlled NER effectively (≥92% aboveground biomass reduction). Amaranthus tuberculatus is the first confirmed weed species in Nebraska to evolve resistance to four distinct herbicide sites of action, leaving no POST herbicide choice for effective control in glyphosate-resistant and conventional (non-transgenic) soybean.

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Prevalence and Mechanism of Atrazine Resistance in Waterhemp (Amaranthus tuberculatus) from Nebraska

Cited by 26 publications

References 54 publications

Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Non-Target-Site Resistance to Herbicides: Recent Developments

Protoporphyrinogen oxidase (PPO) inhibitor–resistant waterhemp (Amaranthus tuberculatus) from Nebraska is multiple herbicide resistant: confirmation, mechanism of resistance, and management

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