Prevalence and Mechanisms of Carbapenem Resistance in <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Systematic Review and Meta-Analysis of Cross-Sectional Studies from Iran

Nasiri, Mohammad Javad; Mirsaeidi, Mehdi; Mousavi, Seyyed Mohammad Javad; Arshadi, Mania; Fardsanei, Fatemeh; Deihim, Behnaz; Davoudabadi, Sara; Zamani, Samin; Hajikhani, Bahareh; Goudarzi, Hossein; Goudarzi, Mehdi; Seghatoleslami, Zahra Sadat; Dabiri, Hossein; Tabarsi, Payam

doi:10.1089/mdr.2019.0440

Cited by 25 publications

(24 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonetheless, according to a meta-analysis study performed by Nasiri et al the rates of CR in E. coli increased from 0.6% in 1997–2000 to 2.9% in 2013–2016 [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

Nasrollahian

Halaji

Hosseini

et al. 2022

International Journal of Clinical Practice

View full text Add to dashboard Cite

Background. Infections due to carbapenem-resistant Enterobacteriaceae (CRE) are associated in patients with urinary catheters alarming rate of emergency status. e aim of this study is to investigate the molecular causes of carbapenem resistance among UPEC as well as antimicrobial resistance trends. Additionally, the potential of isolates to produce biofilms, in addition to their clonal and genetic diversity, was investigated. Material and Methods. A cross-sectional study was accomplished on a collection of 76 non-duplicate UPEC isolates obtained from CAUTIs from May 2021 to September 2021. e modified carbapenem inactivation method (mCIM) and EDTA-modified carbapenem inactivation method (eCIM) test was performed for the detection of carbapenemase and metallo-beta-lactamase activity. Also, the presence of carbapenemase genes was determined using PCR assays. In 96-well microtiter plates, biofilm development was evaluated. ERIC-PCR was used to investigate the clonal and genetic variety of isolates. Results. A total of 76 confirmed UPEC isolates were obtained from patients mentioned to teaching hospitals in Babol, Iran. e results of antibiotic susceptibility testing revealed a high rate of antibiotic resistance against nalidixic acid (81.6%) and trimethoprim-sulfamethoxazole (80.3%). Among UPEC isolates, 63.2% and 13.2% of UPEC isolates were positive for MBL production. e frequencies of the studied genes are in order of bla NDM (14.5%), bla OXA-23 (2.6%), and bla OXA-48 (2.6%). Forty-two isolates (55.3%) were positive for biofilm formation. ERIC-PCR revealed that UPEC isolates could be categorized into nine clusters A-I and five isolates were categorized as a singleton. Conclusion. e high prevalence of MDR and carbapenemaseproducing isolates among the UPEC strain in this investigation is concerning. Moreover, the bla NDM was the most frequent cause of producing metallo-beta-lactamase and carbapenemase. Also, analysis revealed a partial genetic similarity among the studied isolates, indicating that the same UPEC clones may have spread to other hospital units.

show abstract

“…Nonetheless, according to a meta-analysis study performed by Nasiri et al the rates of CR in E. coli increased from 0.6% in 1997–2000 to 2.9% in 2013–2016 [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

Nasrollahian

Halaji

Hosseini

et al. 2022

International Journal of Clinical Practice

View full text Add to dashboard Cite

show abstract

“…The leading cause of infertility is not clear, but it is assumed due to different risk factors, including smoking, alcohol consumption, changes in sexual behaviour, and even infectious diseases, meaningful increase in the prevalence rate of infertility can be observed. Among different infectious risk factors, the role of various viruses has attracted much attention (Abedi et al., 2018; Arefzadeh et al., 2020; Boeri et al., 2019; Garolla et al., 2013; Ghasemnejad et al., 2018; Khajehzadeh et al., 2018; Mahmoudi et al., 2019; Mousavi et al., 2020; Nasiri et al., 2020; Pormohammad et al., 2019; Pourentezari et al., 2016; Schuppe et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

The evaluation of Human papilloma virus and human herpes viruses (EBV, CMV, VZV HSV‐1 and HSV‐2) in semen samples

et al. 2021

Self Cite

View full text Add to dashboard Cite

There are a number of risk factors, especially viral diseases, which can lead to infertility. Among the various viral infections, much attention has been given to the role of the Papillomaviridae and Herpesviridae. After collecting 82 semen samples (37 teratospermia, 2 asthenozoospermia, 2 oligoasthenospermia, 1 oligospermia, 6 asthenoteratospermia and 34 normal semen samples), and washing them, the DNA from both freshly ejaculated spermatozoon and washed spermatozoa was extracted. Subsequently, the prevalence of EBV, CMV, HSV‐1, HSV‐2, VZV and HPV was evaluated using Multiplex PCR and Nested PCR. In this study, 1 normal and 5 abnormal semen samples were infected with HSV‐1 (1 normal, 4 teratospermia and 1 oligoasthenospermia). In addition, there were 2 VZV‐positive samples (both were teratozoospermia). Nested PCR indicated that 1 asthenozoospermia, 1 asthenoteratospermia, 3 teratospermia and 4 normal samples were HPV positive (including 8 HPV‐18 and 1 HPV‐33). Among 9 HPV‐positive subjects, 3 samples were negative after washing the infected samples. The prevalence of EBV, CMV, VZV, HSV‐1 and HSV‐2 remained unchanged prior to and after washing. Maybe sperm washing can be useful to eliminate HPV infection from semen samples, but further investigation is required because of the small number of samples.

show abstract

“…Antibiotic stewardship programs have been associated with reductions in antimicrobial use, duration of stay, and costs with no negative impact on mortality and should be widely promoted in ICUs [5]. [14].…”

Section: Introductionmentioning

confidence: 99%

“…A study that investigated 49 papers found that the rates of resistance to carbapenem in K. pneumoniae and E. coli were 24.0% (95% confidence interval [CI] 18.0-31.0) and 5.0% (95% CI 2.0-8.0), respectively. The blaOXA-48 gene was the most common cause of carbapenem resistance in K. pneumoniae and E. coli [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Rising Klebsiella pneumoniae Infections and Its Expanding Drug Resistance in the Intensive Care Unit of a Tertiary Healthcare Hospital, Saudi Arabia

Bshabshe¹,

Al‐Hakami²,

Alshehri³

et al. 2020

Cureus

View full text Add to dashboard Cite

Nosocomial infections caused by Klebsiella pneumoniae and other Gram-negative organisms have emerged as a significant health problem especially in intensive care units (ICU). This study aims to examine K. pneumoniae infections in the ICU of Aseer Central Hospital and to determine their antimicrobial susceptibility and their relationship to patients' clinical outcomes. This is a retrospective observational study done in a tertiary care center in the Aseer region in Saudi Arabia. The study spanned from January 2018 to December 2019. Demographic, microbiologic, and patient outcomes were collected from 276 patients with various infections. Identification of isolates and in vitro susceptibility to 32 antimicrobial agents were done by the Vitek 2 automated system (bioMérieux, Marcy-l'Étoile, France). Prevalence of K. pneumoniae bacteria, their susceptibility to antimicrobials, and effect on clinical outcome were studied. Two hundred seventy-six K. pneumoniae were recovered from ICU patients with various infections. K. pneumoniae isolates (n=276) were collected mainly from the respiratory tract (61%) and K. pneumoniae represented 39% of the major causal agents of ICU infections, followed by Acinetobacter spp. (30%), Pseudomonas aeruginosa (10.0%), Escherichia coli (7%), and others (14%). The mortality among the 276 ICU patients was 33.3%; K. pneumoniae was connected to 42% of the cases and 67% of the total deaths were between 50 and 90 years of age. K. pneumoniae demonstrated high sensitivity and hence can be recommended for in vivo treatment for tigecycline (81%), cefazolin (77.2%), colistin (64.9%), and to a lesser extent norfloxacin (60%) and imipenem (55.5%). High resistance was detected for ampicillin (100%), extended-spectrum β-lactamases-sulbactam (ESBL-SCM) (100%), piperacillin (100%), and ceftazidime (92.5%). Resistance to carbapenems was elevated in ertapenem (65.2%) and meropenem (61.7%). The increase of K. pneumoniae represents a threat to ICU patients, although K. pneumoniae infections were results rather than the causes, as it was connected to almost half of the ICU mortalities. Tigecycline alone or in combination with colistin on high-dose regimens could be a more effective therapy for treating carbapenem-resistant K. pneumoniae infections.

show abstract

Prevalence and Mechanisms of Carbapenem Resistance in Klebsiella pneumoniae and Escherichia coli: A Systematic Review and Meta-Analysis of Cross-Sectional Studies from Iran

Cited by 25 publications

References 68 publications

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

The evaluation of Human papilloma virus and human herpes viruses (EBV, CMV, VZV HSV‐1 and HSV‐2) in semen samples

Rising Klebsiella pneumoniae Infections and Its Expanding Drug Resistance in the Intensive Care Unit of a Tertiary Healthcare Hospital, Saudi Arabia

Contact Info

Product

Resources

About