Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

Lin, Li; Zhao, Xiujuan; Wu, Xiaowei; Li, Sijing; Liu, Biao; Rajaofera, Mamy Jayne Nelly; Zeng, Yingfei; Dong, Sufang; Bei, Zheng; Peng, Hua; Xia, Qianfeng

doi:10.1186/s12866-021-02246-7

Cited by 8 publications

(9 citation statements)

References 31 publications

(37 reference statements)

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“…The clinical and molecular characteristics of the M. tuberculosis strains vary in different areas. This was observed in this study in consonance with the findings of Liu et al [12]. The diversity of mutations can also provide information for investigating the evolutional lineages of M. tuberculosis.…”

Section: Discussionsupporting

confidence: 93%

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Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

Faye¹,

Hosu²,

Sineke³

et al. 2023

Preprint

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Drug-resistant tuberculosis (DR-TB) remains a major public health problem in South Africa. Gene mu-tations and the genotyping of DR-TB in a rural Eastern Cape Province have not previously been studied. Hence, we sought to detect prevalent mutations linked to DR-TB profiles, identify genetic diversity, and assign lineages. Sputum specimens were obtained from 1157 patients suspected with tuberculosis. They were assayed for rifampin-resistance using Xpert® MTB/RIF.and detection of mutations conferring re-sistance to anti-TB drugs was carried out using GenoType MTBDRplus VER 2.0. Thereafter, 441 isolates were spoligotyped. The most prevalent rifampin resistance-conferring mutations were in rpoB codon S531L. The INH resistant strains, 54.5% had mutations in katG gene at codon S315TB and 24.7% with mutations in inhA gene at codons C-15TB. Furthermore 69.9% strains displayed mutations involving both rpoB and katG genes, while 24.6% strains displayed mutations involving both rpoB and inhA genes. The overall prevalence of heteroresistance was 17.9%. Spoligotyping analysis generated 410 patterns of iso-lates which were found in the international database, with Beijing family being predominant. The diversity of mutations in the study provides information for investigating the evolutional lineages of M. tuberculosis isolates. The recognition frequency of rpoB, katG and inhA mutations in different study areas may help to guide decision‑making about standardization of treatment regimens or individualized treatment in areas where these mutations have been found. This calls for increased drug resistance surveillance and rein-forcement of TB control in this area.

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Section: Discussionsupporting

confidence: 93%

“…Recent research has revealed that different mutations in M. tuberculosis can confer varying levels of phenotypic resistance to anti-TB medications [6,7,11]. Consequently, the aggregation of mutations at several positions has a comprehensive effect on drug resistance [12].…”

Section: Introductionmentioning

confidence: 99%

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

Faye¹,

Hosu²,

Sineke³

et al. 2023

Preprint

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“…In this study, we assessed the associated risk factors for rpoB gene mutation in data sourced areas. The rpoB gene mutation rate of retreated TB patients (83.41%) was higher than that of new cases (62.32%), it has been proved that the RIF resistance rate of retreated tuberculosis is higher than that of new cases in previous studies [ 13 , 14 ], the higher rate may since that patients with retreated pulmonary tuberculosis often fail in the initial treatment due to unreasonable or irregular anti-tuberculosis treatment, resulting in the dominant growth of drug-resistant tuberculosis bacteria, and it’s drug resistance mechanism is related to the mutation of rpoB gene which coding RNA polymerase β-subunit [ 3 ]. Notably, we found no rpoB gene mutation in partial RR-TB strains, but mutations in sensitive strains, the inconsistency between gene resistance and phenotype resistance may be caused by heterogeneity of Mtb.…”

Section: Discussionmentioning

confidence: 97%

The mutation rate of rpoB gene showed an upward trend with the increase of MIRU10, MIRU39 and QUB4156 repetitive number

Cao

Ren

et al. 2023

BMC Genomics

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Background Mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) is a frequently used typing method for identifying the Beijing genotype of Mycobacterium tuberculosis (Mtb), which is easily transformed into rifampicin (RIF) resistance. The RIF resistance of Mtb is considered to be highly related with the mutation of rpoB gene. Therefore, this study aimed to analyze the relationship between the repetitive number of MIRU loci and the mutation of rpoB gene. Methods An open-source whole-genome sequencing data of Mtb was used to detect the mutation of rpoB gene and the repetitive number of MIRU loci by bioinformatics methods. Cochran-Armitage analysis was performed to analyze the trend of the rpoB gene mutation rate and the repetitive number of MIRU loci. Results Among 357 rifampicin-resistant tuberculosis (RR-TB), 304 strains with mutated rpoB genes were detected, and 6 of 67 rifampicin susceptible strains were detected mutations. The rpoB gene mutational rate showed an upward trend with the increase of MIRU10, MIRU39, QUB4156 and MIRU16 repetitive number, but only the repetitive number of MIRU10, MRIU39 and QUB4156 were risk factors for rpoB gene mutation. The Hunter-Gaston discriminatory index (HGDI) of MIRU10 (0.65) and QUB4156 (0.62) was high in the overall sample, while MIRU39 (0.39) and MIRU16 (0.43) showed a moderate discriminatory Power. Conclusion The mutation rate of rpoB gene increases with the addition of repetitive numbers of MIRU10, QUB4156 and MIRU39 loci.

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“…The prevalence of TB in Hainan Province showed no significant seasonality presented in the spatial–temporal distribution study. However, the TB notification rate was relatively higher during spring, possibly due to the climate change and environmental variation [ 7 , 8 ]. The central and southern regions of Hainan Province exhibited higher TB notification rates, which are largely attributed to their higher population density, weak economic conditions, and limited access to medical resources [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Toward tuberculosis elimination by understanding epidemiologic characteristics and risk factors in Hainan Province, China

Zhou,

Li,

et al. 2024

Infect Dis Poverty

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Background The disease burden of tuberculosis (TB) was heavy in Hainan Province, China, and the information on transmission patterns was limited with few studies. This atudy aims to further explore the epidemiological characteristics and influencing factors of TB in Hainan Province, and thereby contribute valuable scientific evidences for TB elimination in Hainan Province. Methods The TB notification data in Hainan Province from 2013 to 2022 were collected from the Chinese National Disease Control Information System Tuberculosis Surveillance System, along with socio-economic data. The spatial–temporal and population distributions were analyzed, and spatial autocorrelation analysis was conducted to explore TB notification rate clustering. In addition, the epidemiological characteristics of the cases among in-country migrants were described, and the delay pattern in seeking medical care was investigated. Finally, a geographically and temporally weighted regression (GTWR) model was adopted to analyze the relationship between TB notification rate and socio-economic indicators. The tailored control suggestions in different regions for TB elimination was provided by understanding epidemiological characteristics and risk factors obtained by GTWR. Results From 2013 to 2022, 64,042 cases of TB were notified in Hainan Province. The estimated annual percentage change of TB notification rate in Hainan Province from 2013 to 2020 was − 6.88% [95% confidence interval (CI): − 5.30%, − 3.69%], with higher rates in central and southern regions. The majority of patients were males (76.33%) and farmers (67.80%). Cases among in-country migrants primarily originated from Sichuan (369 cases), Heilongjiang (267 cases), Hunan (236 cases), Guangdong (174 cases), and Guangxi (139 cases), accounting for 53%. The majority (98.83%) of TB cases were notified through passive case finding approaches, with delay in seeking care. The GTWR analysis showed that gross domestic product per capita, the number of medical institutions and health personnel per 10,000 people were main factors affecting the high TB notification rates in some regions in Hainan Province. Different regional tailored measures such as more TB specialized hospitals were proposed based on the characteristics of each region. Conclusions The notification rate of TB in Hainan Province has been declining overall but still remained high in central and southern regions. Particular attention should be paid to the prevalence of TB among males, farmers, and out-of-province migrant populations. The notification rate was also influenced by economic development and medical conditions, indicating the need of more TB specialized hospitals, active surveillance and other tailored prevention and control measures to promote the progress of TB elimination in Hainan Province.

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Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

Cited by 8 publications

References 31 publications

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

The mutation rate of rpoB gene showed an upward trend with the increase of MIRU10, MIRU39 and QUB4156 repetitive number

Toward tuberculosis elimination by understanding epidemiologic characteristics and risk factors in Hainan Province, China

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