Prevalence and Molecular Characteristics of Vibrio spp. Isolated from Preharvest Shrimp of the North Western Province of Sri Lanka

Koralage, Madura Sanjeevani Gonsal; Alter, Thomas; Pichpol, Duangporn; Strauch, Eckhard; Zessin, Karl-Hans; Huehn, Stephan

doi:10.4315/0362-028x.jfp-12-115

Cited by 28 publications

(28 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study are supported by previous research results, Koralage et al (2012) and Traoré et al (2014) Sumatera and Muara Angke, North Jakarta. Blake et al (1980), Hill et al (2011), and Khariri (2012) found a match between V. cholerae derived from the environment such as seafood and drinking water sources with V. cholerae outbreaks.…”

Section: Discussionsupporting

confidence: 91%

Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia

2019

View full text Add to dashboard Cite

Contamination of pathogenic bacteria in food can lead to the emergence of foodborne disease. One of foodborne disease which often occurs in some developing countries such as Africa, Southeast Asia, and Latin America is cholera which is caused by Vibrio cholerae. The disease is transmitted through beverages and food, especially contaminated seafood. V. cholerae has several virulence factors including the outer membrane protein W (ompW) and cholerae toxin (ctx).The ompW acts as a protective barrier and can also be used as a marker specific species of V. cholerae and cholerae toxin is an enterotoxin responsible for the incidence of diarrhea in a cholera outbreak produced by pathogenic V. cholerae. This study was an observational study to determine the level of contamination of V. cholerae by detecting the outer membrane protein W (ompW) and cholerae toxin subunit A (ctxA) gene of V. cholerae in shrimp and shellfish sold at Kedonganan fish market. Samples were taken using total sampling technique and obtained 24 samples consisting of 14 shrimp samples and 10 shellfish samples. Samples were examined using culture methods and biochemical tests, and then further tested using Duplex Polymerase Chain Reaction (dPCR) to detect ompW and ctxA gene. The dPCR assay results showed 8 out of 14 (57.1%) samples from shrimp and 1 out of 10 (10%) samples from the shellfish positive carried ompW gene, and found no positive samples carrying the ctxA gene in samples derived from shrimp and shellfish. Chi square test analysis results indicated contamination of V. cholerae in shrimp was higher than shellfish based on ompW gene (p<0.05). It can be concluded that the shrimp and shellfish at Kedonganan fish market are contaminated by V. cholerae. Further research is needed to detect the virulence factors besides ompW and ctxA of V. cholerae in seafood.<pre> </pre>Keywords: Foodborne disease, Vibrio cholerae, ompW gene, ctxA gene, and Duplex Polymerase Chain Reaction (dPCR).

show abstract

Section: Discussionsupporting

confidence: 91%

Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia

2019

View full text Add to dashboard Cite

show abstract

“…The strain set consisted of four groups based on the geographic origin of strains and the sampling events: the first group was obtained from prawn farms located in three Sri Lankan regions (n = 43) [30], the second group consists of strains (n = 34) that were isolated from regional and imported food samples in Germany (at retail) of different geographic origins and sample types. Within the third group 27 isolates obtained from local markets and prawn farms in Ecuador are grouped.…”

Section: Methodsmentioning

confidence: 99%

Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity

Urmersbach¹,

Alter²,

Koralage³

et al. 2014

BMC Microbiology

Self Cite

View full text Add to dashboard Cite

BackgroundVibrio parahaemolyticus is frequently isolated from environmental and seafood samples and associated with gastroenteritis outbreakes in American, European, Asian and African countries. To distinguish between different lineages of V. parahaemolyticus various genotyping techniques have been used, incl. multilocus sequence typing (MLST). Even though some studies have already applied MLST analysis to characterize V. parahaemolyticus strain sets, these studies have been restricted to specific geographical areas (e.g. U.S. coast, Thailand and Peru), have focused exclusively on pandemic or non-pandemic pathogenic isolates or have been based on a limited strain number.ResultsTo generate a global picture of V. parahaemolyticus genotype distribution, a collection of 130 environmental and seafood related V. parahaemolyticus isolates of different geographical origins (Sri Lanka, Ecuador, North Sea and Baltic Sea as well as German retail) was subjected to MLST analysis after modification of gyrB and recA PCRs. The V. parahaemolyticus population was composed of 82 unique Sequence Types (STs), of which 68 (82.9%) were new to the pubMLST database. After translating the in-frame nucleotide sequences into amino acid sequences, less diversity was detectable: a total of 31 different peptide Sequence Types (pSTs) with 19 (61.3%) new pSTs were generated from the analyzed isolates. Most STs did not show a global dissemination, but some were supra-regionally distributed and clusters of STs were dependent on geographical origin. On peptide level no general clustering of strains from specific geographical regions was observed, thereby the most common pSTs were found on all continents (Asia, South America and Europe) and rare pSTs were restricted to distinct countries or even geographical regions. One lineage of pSTs associated only with strains from North and Baltic Sea strains was identified.ConclusionsOur study reveals a high genetic diversity in the analyzed V. parahaemolyticus strain set as well as for geographical strain subsets, with a high proportion of newly discovered alleles and STs. Differences between the subsets were identified. Our data support the postulated population structure of V. parahaemolyticus which follows the ‘epidemic’ model of clonal expansion. Application of peptide based AA-MLST allowed the identification of reliable relationships between strains.

show abstract

“…In rare cases, V. parahaemolyticus infection can be fatal [2]. This pathogen is disseminated worldwide and is considered a leading cause of seafood-borne illness in several Asian countries [3,4] and in the United States [5]. For this reason, it is important to understand its many virulence factors and their effects on the human host.…”

Section: Introductionmentioning

confidence: 98%

Vibrio parahaemolyticus virulence determinants

Santos

Salomon

Krachler

et al. 2015

The Comprehensive Sourcebook of Bacterial Protein Toxins

View full text Add to dashboard Cite

Prevalence and Molecular Characteristics of Vibrio spp. Isolated from Preharvest Shrimp of the North Western Province of Sri Lanka

Cited by 28 publications

References 32 publications

Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia

Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia

Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity

Vibrio parahaemolyticus virulence determinants

Contact Info

Product

Resources

About