Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center

Abstract

m e d i c a l j o u r n a l a r m e d f o r c e s i n d i a 7 2 ( 2 0 1 6 ) s 5 4 -s 5Results: Out of 150 CoNS isolates, 51 were methicillin resistant by cefoxitin disk diffusion method. Out of these 51 isolates, mecA gene was detected only in 45 isolates. Moreover, mecA gene was also detected in 4 isolates, which were cefoxitin sensitive. Thus, the prevalence of methicillin resistance among CoNS was found to be 32.7% by PCR. Conclusion: The prevalence of methicillin resistance among Coagulase-negative Staphylococci (CoNS) was 32.7% by PCR detection of mecA gene. The sensitivity and specificity of cefoxitin disk diffusion method against mecA gene detection by PCR were found to be more than 90%. It can be concluded from this study that cefoxitin disk diffusion test can be used as a useful screening method to detect methicillin resistance among CoNS isolates. However, detection of mecA gene by PCR remains a more accurate method of detecting methicillin resistance among CoNS. #

The species distribution, antimicrobial resistance and risk factors for poor outcome of coagulase-negative staphylococci bacteraemia in China

“…This result was similar to research data in southwestern Saudi Arabia [13] and other countries [23, 25]. Not surprisingly, consistent with most previous studies, resistance to vancomycin or linezolid was not found [4, 26]. Multi-drug resistance has made vancomycin the first choice for anti-CoNS, and 25.5% (40/157) of patients were prescribed VAN for empirical antibiotic therapy within 72 h after the onset of CoNS bacteraemia in this study (Table 2).…”

Phenotypic Detection of Β-Lactam Antibiotics, Methicillin and Inducible Clindamycin Resistance Among Bacterial Isolates in Patients with Otitis Externa

“…It is noteworthy to mention that among 14 MDR CoNS isolates, 3 (16.7%) strains were D-zone test positive and 2 (14.3%) strains were resistant to the methicillin group. According to studies conducted by Niedja (Brazil) and Bhatt (India), none of the CoNS isolates were D-zone test positive (5,27).…”

Methicillin Resistant Staphylococci Isolated in Clinical Samples: a 3-year Retrospective Study Analysis

“…A report from Congo demonstrated a 63.5% prevalence of MRS among hospitalized surgical patients with 63.5 and 60% being MRSA and MR-CNS, respectively [9]. Likewise, a 4-year cross-sectional study conducted in India revealed a 34% prevalence of MRS from clinical specimens [10], while Ramsamy et al showed a decreasing MRSA prevalence ranging from 28% to 18% over a 5-year period (2011)(2012)(2013)(2014)(2015) in KwaZulu-Natal, South Africa [11]. In contrast to low-and-middle-income countries, lower MRSA prevalence (<25%) has been reported in European countries [2].…”