Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Beijing, China

Li, Fuhuang; Wang, Haiyan; Zhang, Zhenjie; Li, Junqiang; Wang, Chenrong; Zhao, Jinfeng; Hu, Suhui; Wang, Rongjun; Zhang, Longxian; Wang, Ming

doi:10.1016/j.vetpar.2016.01.023

Cited by 52 publications

(43 citation statements)

References 47 publications

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“…Three species of Cryptosporidium (28 C. bovis , 7 C. parvum [subtype IIdA15G1] and 5 C. ryanae ) were identified in this study. Previous studies have shown that C. parvum is a major species in pre-weaned calves in Beijing [10], Xinjiang [17] and Ningxia [8], whereas C. bovis predominated in pre-weaned calves in this study, similar to reports from Henan [27] and Heilongjiang [32]. …”

Section: Resultssupporting

confidence: 89%

“…Prevalence of Cryptosporidium was significantly different ( X 2 = 4.924, df = 1, p = 0.026) between intensive feeding and free-ranging farms in this study, which suggests that cultivation scale may lead to differences in infection rates with Cryptosporidium . Other studies also showed that geographic distribution and host health status may lead to the difference [5, 10, 17]. Three species of Cryptosporidium (28 C. bovis , 7 C. parvum [subtype IIdA15G1] and 5 C. ryanae ) were identified in this study.…”

Section: Resultssupporting

confidence: 55%

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Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 55%

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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“…Subtype IIdA20G1 was found in Heilongjiang Province [11]. The rest of subtypes, IIdA14G1, IIdA17G1, IIdA18G1, IIdA21G1 was only detected in Xinjiang [9], Beijing [23], Tibet [27], and Shandong (data unpublished), respectively. The small subunit rRNA (SSU rRNA) gene was used to identify the Cryptosporidium species with primers and under the reaction conditions described previously [28].…”

Section: Discussionmentioning

confidence: 92%

“…Gansu Provinces [9,11,12,[17][18][19]23]. Subtype IIdA19G1 was mostly found in Jiangsu, Henan, Shanghai, Xinjiang, Guangdong, Hebei, Tianjin, Beijing, and Heilongjiang Provinces [10,14,15,[21][22][23]25].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Cryptosporidium parvum in neonatal calves in Xinjiang, China

Zhang

Wei

et al. 2019

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Background The Cryptosporidium causes infection in wide spectrum of vertebrate hosts and is well known for leading to diarrhea and other gastrointestinal illness. To assess Cryptosporidium genetic diversity in neonatal calves and probe the cause for diarrhea of them, a total of 232 fecal samples from neonatal calves in 12 farms in Xinjiang were characterized for the presence of Cryptosporidium .Results The prevalence of Cryptosporidium was 38.4% (89/232), and three species were detected with SSU rRNA gene, including C. parvum (n = 88), C. ryanae (n = 9), and C. bovis (n = 1). Prevalence of C . parvum neonatal calves with diarrhea (52.6%, 51/97) was significantly higher than calves without diarrhea (28.1%, 38/135). All the C . parvum -positive samples were analyzed based on gp60 gene, IIdA15G1 (n = 35), IIdA20G1 (n = 21), IIdA14G1 (n = 17), and IIdA19G1 (n = 13) were successfully subtyped in this study.Conclusions These data indicated that C . parvum was a major contributor in diarrheal disease in neonatal calves, and C . parvum subtypes from neonatal calves in Xinjiang were high genetic diversity. Additionally, our findings implicating neonatal calves could be a potential source of human Cryptosporidium infection and provide further evidence for the uniqueness of C . parvum IId subtypes in cattle in China.

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“…Whether C. parvum or C. bovis is the dominant Cryptosporidium species in pre-weaned dairy calves is controversial among publications that document Cryptosporidium prevalence among cattle in China. Cryptosporidium bovis was more common in central China (Henan and Hubei) [14,25], eastern China (Shanghai) [27], southwestern China (Sichuan) [26], and southern China (Guangdong) [15,28], whereas C. parvum is more common in northeastern China (Heilongjiang) [32], northern China (Beijing, Hebei, and Tianjin) [20,33], and most of northwestern China (Ningxia and Xinjiang) [10,22,24]. Dairy calves are most commonly infected with C. parvum within 1 month of birth, whereas C. bovis is mainly associated with dairy calves aged 2-3 months [6].…”

Section: Discussionmentioning

confidence: 99%

Seasonal monitoring of Cryptosporidium species and genetic diversity in neonatal dairy calves on two large-scale farms in Xinjiang, China

Zhang

Jing

et al. 2020

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Background: Neonatal dairy calves infected with Cryptosporidium can possess a significant source of zoonotic infections and disease. To assess seasonal variations in the prevalence and genetic diversity of Cryptosporidium in neonatal dairy calves, 380 fecal samples from neonatal dairy calves on two large-scale farms in Xinjiang (Alaer and Wensu) were screened for the Cryptosporidium small subunit (SSU) rRNA gene. Results: The overall prevalence of Cryptosporidium was 48.7% (185/380): 48.6% (108/222) in Alaer and 48.7% (77/158) in Wensu. Cryptosporidium was most frequent in summer (56.8%, 54/95), followed by spring (50.0%, 44/88), winter (46.8%, 44/94), and autumn (41.7%, 43/103) (P > 0.05). Cryptosporidium was significantly more prevalent in calves with diarrhea (72.4%, 113/156) than in those without (32.1%, 72/224) (P < 0.01). Based on a restriction fragment length polymorphism (RFLP) analysis, C. parvum (n = 173), C. bovis (n = 7), C. ryanae (n = 3), and co-infections of the three species (n = 2) were identified. Most (172/175) C. parvum samples were successfully sequenced at the 60-kDa glycoprotein gene (gp60), revealing two zoonotic subtypes: IIdA14G1 (n = 94) and IIdA15G1 (n = 7) in Alaer and IIdA15G1 (n = 71) in Wensu.Conclusions: These results showed that neonatal dairy calves were commonly infected with Cryptosporidium throughout the year, and there was a significant association between the occurrence of diarrhea and Cryptosporidium infection. Presence of IIdA14G1 and IIdA15G1 indicated neonatal dairy calves may be a source of zoonotic C. parvum subtypes.

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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Beijing, China

Cited by 52 publications

References 47 publications

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Genetic diversity of Cryptosporidium parvum in neonatal calves in Xinjiang, China

Seasonal monitoring of Cryptosporidium species and genetic diversity in neonatal dairy calves on two large-scale farms in Xinjiang, China

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