Prevalence and molecular characterization of Giardia duodenalis isolates from dairy cattle in northeast China

Liu, Gang; Su, Yan; Zhou, Mengjiao; Zhao, Jidong; Zhang, Tianyu; Ahmad, Waqas; Lu, Huijun; Jiang, Ning; Chen, Qijun; Xiang, Ming; Yin, Jun

doi:10.1016/j.exppara.2015.03.020

Cited by 33 publications

(23 citation statements)

References 47 publications

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“…In this study, the overall infection rate in southwestern China was close to the prevalence in northwestern (9.7% [18]), northeastern (13.3% [13]) and north China (7.1% [7]), but much lower than the infection rates in central (17.6% [26]) and southeastern (60.1% [29]) China. Prior to the present study, these results were interpreted as related to differences in geographic distribution, environmental management and cultivation scale [7, 13, 18, 26, 29]. Cattle were kept in groups or in free stalls, which might promote the transmission of G. duodenalis infection among animals and lead to the high infection rates [26, 29].…”

Section: Resultssupporting

confidence: 51%

“…Its prevalence shows substantial differences, ranging from 7.1% to 60.1% in other studies in China [7, 13, 18, 26, 29]. In this study, the overall infection rate in southwestern China was close to the prevalence in northwestern (9.7% [18]), northeastern (13.3% [13]) and north China (7.1% [7]), but much lower than the infection rates in central (17.6% [26]) and southeastern (60.1% [29]) China. Prior to the present study, these results were interpreted as related to differences in geographic distribution, environmental management and cultivation scale [7, 13, 18, 26, 29].…”

Section: Resultsmentioning

confidence: 96%

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Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China.

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 96%

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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“…In humans, G. duodenalis can infect immunocompromised hosts [ 21 ], such as AIDS patients, and can be life-threatening. G. duodenalis has been reported in livestock [ 12 , 16 , 26 ], wildlife [ 13 , 20 ], and companion animals [ 10 , 11 , 15 , 27 ]; it infects numerous mammalian species, including humans and species that are closely connected with humans. It is distributed worldwide and poses a threat to public health.…”

Section: Introductionmentioning

confidence: 99%

“…Assemblages C and D are observed in canines [ 10 , 11 , 27 ]. Assemblage E has been reported in cattle [ 12 ]. Assemblage F is specific to cats, pigs, and cetaceans [ 1 , 15 , 20 ], and assemblage G is specific to mice and rats [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China

et al. 2017

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Giardia duodenalis (also known as G. intestinalis) is a flagellated protozoan that parasitizes the small intestine and is a common causal agent of zoonotic infections in humans and animals. To assess the genetic diversity and zoonotic transmission potential of G. duodenalis in stray dogs, 159 fecal specimens were collected from dogs in Chengdu, Yaan, and Leshan in Sichuan province, China. Of the 159 fecal samples from stray dogs, 18 (11.3%) were G. duodenalis-positive based on nested PCR amplification of the beta giardin (bg) gene, and the occurrence varied from 1.8% to 35% in different cities. Dog-specific assemblages C (n = 9) and D (n = 9) were identified. The glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes of all bg-positive isolates were characterized. A total of 16 and 8 isolates were positive for the gdh and tpi genes, respectively. Two novel sequences of the bg locus were detected among genetic assemblage D isolates, and one novel gdh sequence and four novel tpi sequences were identified among genetic assemblage C isolates. Mixed infections of assemblages C and D were also detected. Assemblages A and B, which have high zoonotic potential, were not detected. Our results show that G. duodenalis is prevalent and a cause of diarrhea in dogs in Sichuan province, China.

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“…duodenalis is an important intestinal pathogen of livestock. The number of studies on the prevalence and molecular characterization of G. duodenalis in cattle in different parts of the world is increasing (Coklin et al, 2007;Gillhuber et al, 2013;Huang et al, 2014;Liu et al, 2015). However, only limited studies have been performed in Turkey on giardiosis in calves, with prevalence rates between 4.1% and 14.7% (Degerli et al, 2005;Goz et al, 2006;Gul et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

De werkzaamheid van chloroquinebehandeling van Giardia duodenalis-infectie bij kalveren

Gültekin¹,

Ural²,

Aysul³

et al. 2016

Vlaams Diergeneeskundig Tijdschrift

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The purpose of the present study was to evaluate the effect of chloroquine treatment on cyst excretion in calves naturally infected with Giardia duodenalis. The calves were randomly assigned into two groups based on placebo (group I, n=7 untreated control calves) or treatment (group II, n=7 calves treated orally with 2.5 mg/kg chloroquine twice daily for five consecutive days). The G. duodenalis isolates were identified by molecular characterization with β-giardin nested PCR and gene sequence analysis as assemblage A3. Cyst excretion was determined on days 0, 3, 7 and 10, before and after treatment. Geometric means of the number of excreted cysts did not change significantly in the control group during the trial. The reduction in cyst excretion after chloroquine treatment was 99% on day 3 and 100% on days 7 and 10. Chloroquine treatment is most probatly practically applicable, relatively inexpensive and highly effective against giardiosis in calves.

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Prevalence and molecular characterization of Giardia duodenalis isolates from dairy cattle in northeast China

Cited by 33 publications

References 47 publications

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Detection and multilocus genotyping of Giardia duodenalis in dogs in Sichuan province, China

De werkzaamheid van chloroquinebehandeling van Giardia duodenalis-infectie bij kalveren

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