Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

Abstract

This study was performed to determine the prevalence and genotypes of Cryptosporidium species among HIV patients and cattle in Thailand. Stool specimens were collected from 46 HIV patients from Prabat Nampu Temple, Lop Buri Province in central Thailand. Two hundred fecal samples from dairy cattle were collected from seven farms in Chon Buri Province, the eastern part of Thailand. Each sample was concentrated by Sheather's sucrose flotation technique and stained by acid fast stain (AFS) for the identification of oocysts by microscopy. All HIV stool samples and 83 fecal specimens from cattle were further tested using nested polymerase chain reaction (PCR) targeting the 18S SSUrRNA gene to characterize the detected species. In HIV patient samples, the detection rate was 28.7% by AFS and 4.35% by nested PCR. In cattle samples, the detection rate was 13% by AFS and 9.63% by nested PCR. After DNA sequencing results, we identified the genotypes of the Cryptosporidium from seven of the PCR positive samples. All were found to be C. parvum. The findings presented here represent the first genetic identification of Cryptosporidium species in cattle in Thailand.

Prevalence of Cryptosporidium and Giardia in the water resources of the Kuang River catchment, Northern Thailand

“…Kaewthamasorn and Wongsamee (2006) screened 207 faecal samples of beef cattle in Nan Province, Northern Thailand but did not detect either parasite in their samples. While we did not detect either Cryptosporidium or Giardia in faecal samples of dairy cattle at our study site, other Thai studies have (Inpankaew et al, 2010;Jittapalapong et al, 2006Jittapalapong et al, , 2011Nuchjangreed et al, 2008). In Northern Thailand, the seroprevalence of Cryptosporidium parvum infection of 642 dairy cows were 3.3%, 5.1% and 3.0% in the provinces of Chiang Mai, Chiang Rai and Lampang, respectively (Inpankaew et al, 2009).…”

Cryptosporidium and Giardia detection in environmental waters of southwest coastal areas of Thailand

“…in HIVinfected patients, in cattle, and in food (green mussels) have been already reported from Thailand (Uga et al 2000;Gatei et al 2002;Saksirisampant et al 2002;Tiangtip and Jongwutiwes 2002;Nuchjangreed et al 2008;Srisuphanunt et al 2009), no outbreaks of waterborne cryptosporidiosis or giardiasis have been reported in the country, and they could not be included in the systematic review covering all reports worldwide of waterborne protozoan outbreaks by Karanis et al (2007). No data exist regarding protozoan parasite concentrations in wastewaters or in reuse and recycling of wastewaters for agriculture and industry and in backwash water from drinking water treatment plants in Thailand.…”

Prevalence and molecular characterization ofCryptosporidiumspp. in dairy cattle from farms in China

“…Since then, there have been numerous reports of cryptosporidiosis in different countries and regions [5,6,17,19,20,27]. However, only a few publications have documented Cryptosporidium prevalence among animals in China [7,40].…”